公开(公告)号：US20100136610A1

公开(公告)日：2010-06-03

申请号：US12629797

申请日：2009-12-02

申请人： Jeong-Yeol Yoon ,  Jae-Young Song

发明人： Jeong-Yeol Yoon ,  Jae-Young Song

IPC分类号： C12Q1/04 ,  C12M1/34

CPC分类号： G01N33/569 ,  G01N33/54313 ,  Y02A50/59

摘要： The present invention features a microfluidic device for detecting Escherichia coli. The device comprises (a) a base slide having a first inlet and a second inlet, both of which connect at a vertex, where the first inlet is for accepting beads conjugated with anti-E. coli and the second inlet is for accepting a sample, wherein at the vertex the beads conjugated with anti-E. coli and the sample combine to form a combined mixture; (b) a portable spectrometer and a light source; and (c) a first fiber optic cable for directing an incident light into the combined mixture and a second fiber optic cable for detection of light scattering from the combined mixture, where the fiber optic cables are arranged in a proximity fiber arrangement, with the second fiber positioned above the base slide so as to detect forward light scattering at about a 45° angle.

摘要翻译： 本发明的特征在于用于检测大肠杆菌的微流体装置。 该装置包括（a）具有第一入口和第二入口的基底滑块，两者都在顶点连接，其中第一入口用于接收与抗E缀合的珠粒。 大肠杆菌和第二入口用于接受样品，其中在顶点处缀合有抗E的珠。 大肠杆菌和样品结合形成混合物; （b）便携式光谱仪和光源; 和（c）用于将入射光引导到组合混合物中的第一光纤电缆和用于检测来自组合混合物的光散射的第二光纤电缆，其中光纤电缆布置在接近光纤配置中，第二光纤电缆 纤维定位在基底滑块上方，以便以大约45°的角度检测向前的光散射。