公开(公告)号：US06452068B1

公开(公告)日：2002-09-17

申请号：US09439535

申请日：1999-11-12

申请人： Jianru Zuo ,  Qiwen Niu ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qiwen Niu ,  Nam-Hai Chua

IPC分类号： C12N1582

CPC分类号： C12N15/8295 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8238 ,  C12N15/8242

摘要： Chemically inducible promoters are described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoters described are ones that are inducible by a glucocorticoid or estrogen which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt, CKI1, or knotted1, to induce shoot formation in the presence of an appropriate inducer. The promoters may be used with genes which induce somatic embryos such as Lec1 or SERK to prepare somatic embryos which can be grown into seedlings and then into plants. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.

摘要翻译： 描述了可用于通过将植物或植物细胞添加到含有启动子诱导剂的培养基的培养基中或通过从其中除去植物或植物细胞而易于调节的基因来转化植物（包括烟草和莴苣）的化学诱导型启动子 中。 所描述的启动子是由糖皮质激素或雌激素诱导的不是植物内源性的启动子。 这样的启动子可以与各种基因如ipt，CKI1或打结的1一起使用，以在合适的诱导物存在下诱导芽形成。 启动子可以与诱导体细胞胚如Lec1或SERK的基因一起使用，以制备体细胞胚，其可以生长成幼苗，然后进入植物。 启动子也可以与抗生素或除草剂抗性基因一起使用，然后通过存在或不存在诱导物而不是组成型的基因。 还可以列举可置于诱导型启动子控制下的基因的其他实例。

公开(公告)号：US07525012B2

公开(公告)日：2009-04-28

申请号：US10196416

申请日：2002-07-17

申请人： Jianru Zuo ,  Qiwen Niu ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qiwen Niu ,  Nam-Hai Chua

IPC分类号： C12N15/82 ,  C12N5/00 ,  C12N15/63

CPC分类号： C12N15/8295 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8238 ,  C12N15/8242

摘要： Chemically inducible promoters are described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoters described are ones that are inducible by a glucocorticoid or estrogen which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt, CKI1, or knotted1, to induce shoot formation in the presence of an appropriate inducer. The promoters may be used with genes which induce somatic embryos such as Lec1 or SERK to prepare somatic embryos which can be grown into seedlings and then into plants. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.