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公开(公告)号：US20110124101A1
公开(公告)日：2011-05-26
申请号：US12866127
申请日：2009-02-06
CPC分类号： C12P19/34 , B03D1/028 , B03D1/082 , B03D1/1462 , B03D1/1468 , B03D1/1487 , B03D1/18 , B03D2203/003 , C12N15/1003 , C12N15/1006
摘要： A scalable process and device for producing a bio molecule, in particular pharmaceutical grade plasmid DNA is described. The process includes the steps of alkaline lysis, neutralization and clarification and can be further extended. For separating the lysate and the precipitate an improved floatation method is disclosed. This method is based on attachment of CO2 bubbles on the precipitate floe. The CO2 is released from a carbonate salt during or after neutralization (acidification). The method of the invention is preferably carried out in an automated continuous mode applying devices for lysis and neutralization and a novel device for completely continuous clarification (separation of floes and clarified lysate).
摘要翻译： 描述了用于生产生物分子，特别是药物级质粒DNA的可扩展的方法和装置。 该方法包括碱裂解，中和和澄清的步骤，并可进一步扩展。 为了分离裂解物和沉淀物，公开了改进的浮选方法。 这种方法是基于沉淀物上的二氧化碳气泡的附着。 在中和（酸化）期间或之后，CO 2从碳酸盐中释放出来。 本发明的方法优选以自动连续模式施加用于裂解和中和的装置和用于完全连续澄清（分离絮凝物和澄清裂解物）的新装置进行。
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公开(公告)号：US08501402B2
公开(公告)日：2013-08-06
申请号：US10806346
申请日：2004-03-23
申请人： Jochen Urthaler , Roman Necina , Christine Ascher , Helga Woehrer
发明人： Jochen Urthaler , Roman Necina , Christine Ascher , Helga Woehrer
CPC分类号： C12N15/1006
摘要： A scalable process and device for producing a biomolecule, in particular pharmaceutical grade plasmid DNA. The process includes the steps of alkaline lysis and a neutralization. For separating the lysate and the precipitate, the mixture is allowed to gently flow downward through a clarification reactor that is partially filled, in its lower part, with retention material like glass beads, whereby the precipitate is retained on top of and within the retention. In a preferred embodiment of the lysis step, cell suspension and alkaline lysis solution flow through a lysis reactor that is filled with particulate material like glass beads. The process can be run continuously and fully automated.
摘要翻译： 用于生产生物分子，特别是药物级质粒DNA的可扩展的方法和装置。 该方法包括碱裂解和中和的步骤。 为了分离裂解物和沉淀物，将混合物轻轻地向下流过澄清反应器，澄清反应器在其下部部分地填充有诸如玻璃珠的保留材料，由此沉淀物保留在保留物的顶部和内部。 在裂解步骤的优选实施方案中，细胞悬浮液和碱性裂解溶液流过填充有诸如玻璃珠的颗粒材料的裂解反应器。 该过程可以连续运行并完全自动化。
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公开(公告)号：US20050026177A1
公开(公告)日：2005-02-03
申请号：US10806346
申请日：2004-03-23
申请人： Jochen Urthaler , Roman Necina , Christine Ascher , Helga Woehrer
发明人： Jochen Urthaler , Roman Necina , Christine Ascher , Helga Woehrer
CPC分类号： C12N15/1006
摘要： A scalable process and device for producing a biomolecule, in particular pharmaceutical grade plasmid DNA. The process includes the steps of alkaline lysis and a neutralization. For separating the lysate and the precipitate, the mixture is allowed to gently flow downward through a clarification reactor that is partially filled, in its lower part, with retention material like glass beads, whereby the precipitate is retained on top of and within the retention. In a preferred embodiment of the lysis step, cell suspension and alkaline lysis solution flow through a lysis reactor that is filled with particulate material like glass beads. The process can be run continuosly and fully automated.
摘要翻译： 用于生产生物分子，特别是药物级质粒DNA的可扩展的方法和装置。 该方法包括碱裂解和中和的步骤。 为了分离裂解物和沉淀物，将混合物轻轻地向下流过澄清反应器，澄清反应器在其下部部分地填充有诸如玻璃珠的保留材料，由此沉淀物保留在保留物的顶部和内部。 在裂解步骤的优选实施方案中，细胞悬浮液和碱性裂解溶液流过填充有诸如玻璃珠的颗粒材料的裂解反应器。 该过程可以连续运行并完全自动化。
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