公开(公告)号：US06881539B1

公开(公告)日：2005-04-19

申请号：US09622353

申请日：1999-02-16

申请人： John A. Arbuckle ,  Timothy W. Fox ,  Robert B. Meeley ,  Benjamin A. Bowen ,  John A. McElver

发明人： John A. Arbuckle ,  Timothy W. Fox ,  Robert B. Meeley ,  Benjamin A. Bowen ,  John A. McElver

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12N15/1065 ,  C12N15/1079 ,  C12N15/1082

摘要： A method for the rapid isolation and identification of the DNA sequence of transposable element-tagged genes is provided. The method comprises a modified AFLP approach using a transposable element-anchored amplification to identify and clone an amplification product that is associated with a mutant phenotype. Once cloned, the amplification product of interest may be used to screen a cDNA library directly or may be sequenced and compared to available databases for sequence homology. A modification of this approach provides a method for the identification of the location of an additional type of insertion event into genomic DNA, more specifically transgene insertion into the genome of a host organism.

摘要翻译： 提供了用于快速分离和鉴定转座元件标记基因的DNA序列的方法。 该方法包括使用可转座元件锚定扩增来鉴定和克隆与突变表型相关的扩增产物的修饰AFLP方法。 一旦被克隆，可以使用目的扩增产物直接筛选cDNA文库或进行测序，并与序列同源性的可用数据库进行比较。 该方法的修改提供了用于将额外类型的插入事件的位置识别到基因组DNA中的方法，更具体地说是将基因插入到宿主生物体的基因组中。