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12 条记录 (耗时 0.034 秒)

    Transposable element-anchored, amplification method for isolation and identification of tagged genes
    1.
    发明授权
    Transposable element-anchored, amplification method for isolation and identification of tagged genes 失效
    可转移元件锚定，用于分离和鉴定标记基因的扩增方法

    公开(公告)号：US06881539B1

    公开(公告)日：2005-04-19

    申请号：US09622353

    申请日：1999-02-16

    申请人： John A. Arbuckle ,  Timothy W. Fox ,  Robert B. Meeley ,  Benjamin A. Bowen ,  John A. McElver

    发明人： John A. Arbuckle ,  Timothy W. Fox ,  Robert B. Meeley ,  Benjamin A. Bowen ,  John A. McElver

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12N15/1065 ,  C12N15/1079 ,  C12N15/1082

    摘要： A method for the rapid isolation and identification of the DNA sequence of transposable element-tagged genes is provided. The method comprises a modified AFLP approach using a transposable element-anchored amplification to identify and clone an amplification product that is associated with a mutant phenotype. Once cloned, the amplification product of interest may be used to screen a cDNA library directly or may be sequenced and compared to available databases for sequence homology. A modification of this approach provides a method for the identification of the location of an additional type of insertion event into genomic DNA, more specifically transgene insertion into the genome of a host organism.

    摘要翻译： 提供了用于快速分离和鉴定转座元件标记基因的DNA序列的方法。 该方法包括使用可转座元件锚定扩增来鉴定和克隆与突变表型相关的扩增产物的修饰AFLP方法。 一旦被克隆，可以使用目的扩增产物直接筛选cDNA文库或进行测序，并与序列同源性的可用数据库进行比较。 该方法的修改提供了用于将额外类型的插入事件的位置识别到基因组DNA中的方法，更具体地说是将基因插入到宿主生物体的基因组中。

    Functional characterization of genes
    2.
    发明授权
    Functional characterization of genes 失效
    基因的功能表征

    公开(公告)号：US5962764A

    公开(公告)日：1999-10-05

    申请号：US835638

    申请日：1997-04-10

    申请人： Steven P. Briggs ,  Robert B. Meeley

    发明人： Steven P. Briggs ,  Robert B. Meeley

    IPC分类号： A01H1/04 ,  C12N15/82 ,  C12Q1/68 ,  A01H4/00 ,  C12N15/09

    CPC分类号： C12N15/8241 ,  A01H1/04 ,  C12Q1/6895

    摘要： Insertions into a gene of known sequence can be generated by crossing two parent plants, one of which contains a transposable element, to produce F.sub.1 progeny plants in which the insertion is detected by means of a PCR. F.sub.1 progeny plants containing such an insertion are self-fertilized to produce F.sub.2 progeny which are homozygous for the insertion. The function of a gene disabled by the insertion can be ascertained from a comparison of the phenotype of the F.sub.2 progeny with a parental phenotype. Large numbers of F.sub.1 progeny can be tested simultaneously for the presence of insertions. A collection of F.sub.2 seed can be stored and used for phenotype comparison when an insertion is detected.

    摘要翻译： 可以通过将两个亲本植物（其中一个含有转座因子）杂交到产生F1后代植物中来产生已知序列的基因，其中通过PCR检测插入。 含有这种插入物的F1后代植物是自我受精的，以产生纯合插入的F2后代。 可以从F2后代的表型与亲本表型的比较中确定插入能够阻断的基因的功能。 可以同时测试大量的F1后代的插入物的存在。 当检测到插入时，可以存储F2种子的集合并用于表型比较。

    Plants transformed with a DNA construct comprising a nucleic acid molecule encoding an 18 kD α-globulin
    8.
    发明授权
    Plants transformed with a DNA construct comprising a nucleic acid molecule encoding an 18 kD α-globulin 失效
    用包含编码18kDα-球蛋白的核酸分子的DNA构建体转化的植物

    公开(公告)号：US06858778B1

    公开(公告)日：2005-02-22

    申请号：US10053410

    申请日：2001-11-07

    申请人： Rudolf Jung ,  Wang-Nan Hu ,  Robert B. Meeley ,  Vincent J. H. Sewalt

    发明人： Rudolf Jung ,  Wang-Nan Hu ,  Robert B. Meeley ,  Vincent J. H. Sewalt

    IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  C12N15/11 ,  A01H5/00

    CPC分类号： C12N15/8253 ,  C07K14/415 ,  C12N15/8251 ,  C12N15/8254

    摘要： The present invention is directed to a composition for altering the levels of seed proteins in cereal grain. The invention is directed to the alteration of seed protein levels in plant grain, resulting in grain with increased digestibility/nutrient availability, improved amino acid composition/nutritional value, increased response to feed processing, improved silage quality, and increased efficiency of wet or dry-milling. The composition provided comprises a nucleotide sequence encoding a maize 18 kD alpha-globulin cDNA as well as expression cassettes comprising nucleotide sequences of the invention. Also provided are transformed plant tissue including plants, cells and seeds thereof.

    摘要翻译： 本发明涉及一种用于改变谷粒中种子蛋白质水平的组合物。 本发明涉及植物籽粒中种子蛋白质水平的改变，导致谷物具有增加的消化率/营养物质利用度，改善的氨基酸组成/营养价值，增加对饲料加工的反应，改善青贮饲料质量以及提高湿或干的效率 碾碎 提供的组合物包含编码玉米18kDα-球蛋白cDNA的核苷酸序列以及包含本发明的核苷酸序列的表达盒。 还提供了转化的植物组织，包括植物，细胞和种子。