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1.发明申请公开(公告)号：US20110046201A1
公开(公告)日：2011-02-24
申请号：US12112803
申请日：2008-04-30
IPC分类号： A61K31/713 , C12P19/34 , C12N5/10 , C12N15/63
CPC分类号： C12P19/34 , C07H21/04 , C12N9/90 , C12N15/10 , C12N15/111 , C12N15/64 , C12N15/66 , C12N15/86 , C12N2310/111 , C12N2310/14 , C12N2330/30 , C12N2740/16043 , C12N2800/30 , C12N2800/70 , C12Y599/01
摘要： The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention.
摘要翻译： 本发明涉及生物技术和分子生物学领域。 更具体地说，本发明涉及克隆或亚克隆一个或多个包含一个或多个II型限制酶识别位点的核酸分子。 本发明还使用重组克隆方法（例如使用重组位点和重组蛋白的那些）来体现克隆这种核酸分子。 本发明还涉及由使用本发明的方法生产的宿主细胞表达的核酸分子（包括RNA和iRNA）以及蛋白质。
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公开(公告)号：US20120149069A1
公开(公告)日：2012-06-14
申请号：US13401680
申请日：2012-02-21
IPC分类号： C12N15/66
CPC分类号： C12P19/34 , C07H21/04 , C12N9/90 , C12N15/10 , C12N15/111 , C12N15/64 , C12N15/66 , C12N15/86 , C12N2310/111 , C12N2310/14 , C12N2330/30 , C12N2740/16043 , C12N2800/30 , C12N2800/70 , C12Y599/01
摘要： The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention.
摘要翻译： 本发明涉及生物技术和分子生物学领域。 更具体地说，本发明涉及克隆或亚克隆一个或多个包含一个或多个II型限制酶识别位点的核酸分子。 本发明还使用重组克隆方法（例如使用重组位点和重组蛋白的那些）来体现克隆这种核酸分子。 本发明还涉及由使用本发明的方法生产的宿主细胞表达的核酸分子（包括RNA和iRNA）以及蛋白质。
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3.发明授权公开(公告)号：US08338091B2
公开(公告)日：2012-12-25
申请号：US13401680
申请日：2012-02-21
申请人： Jonathan Chesnut , Miroslav Dudas , Adam Harris , Louis Leong , Knut Madden
发明人： Jonathan Chesnut , Miroslav Dudas , Adam Harris , Louis Leong , Knut Madden
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C07H21/04 , C12N9/90 , C12N15/10 , C12N15/111 , C12N15/64 , C12N15/66 , C12N15/86 , C12N2310/111 , C12N2310/14 , C12N2330/30 , C12N2740/16043 , C12N2800/30 , C12N2800/70 , C12Y599/01
摘要： The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention.
摘要翻译： 本发明涉及生物技术和分子生物学领域。 更具体地说，本发明涉及克隆或亚克隆一个或多个包含一个或多个II型限制酶识别位点的核酸分子。 本发明还使用重组克隆方法（例如使用重组位点和重组蛋白的那些）来体现克隆这种核酸分子。 本发明还涉及由使用本发明的方法生产的宿主细胞表达的核酸分子（包括RNA和iRNA）以及蛋白质。
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4.发明申请公开(公告)号：US20050069929A1
公开(公告)日：2005-03-31
申请号：US10913501
申请日：2004-08-09
申请人： Jonathan Chestnut , Knut Madden , Miroslav Dudas , Louis Leong , Adam Harris
发明人： Jonathan Chestnut , Knut Madden , Miroslav Dudas , Louis Leong , Adam Harris
IPC分类号： A61K48/00 , C07H21/04 , C12N20060101 , C12N15/10 , C12N15/11 , C12N15/64 , C12N15/66 , C12N15/867 , C12P19/34 , C12Q1/68
CPC分类号： C12P19/34 , C07H21/04 , C12N9/90 , C12N15/10 , C12N15/111 , C12N15/64 , C12N15/66 , C12N15/86 , C12N2310/111 , C12N2310/14 , C12N2330/30 , C12N2740/16043 , C12N2800/30 , C12N2800/70 , C12Y599/01
摘要： The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention.
摘要翻译： 本发明涉及生物技术和分子生物学领域。 更具体地说，本发明涉及克隆或亚克隆一个或多个包含一个或多个II型限制酶识别位点的核酸分子。 本发明还使用重组克隆方法（例如使用重组位点和重组蛋白的那些）来体现克隆这种核酸分子。 本发明还涉及由使用本发明的方法制备的宿主细胞表达的核酸分子（包括RNA和iRNA）以及蛋白质。
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5.发明申请METHODS AND COMPOSITIONS FOR SYNTHESIS OF NUCLEIC ACID MOLECULES USING MULTIPLERECOGNITION SITES 审中-公开使用多重分解位点合成核酸分子的方法和组合物公开(公告)号：US20130004996A1
公开(公告)日：2013-01-03
申请号：US13219440
申请日：2011-08-26
申请人： Jonathan D. CHESNUT , John Carrino , Louis Leong , Knut Madden , Martin A. G. Gleeson , James Fan , Michael A. Brasch , David Cheo , James L. Hartley , Devon R.N. Byrd , Gary F. Temple
发明人： Jonathan D. CHESNUT , John Carrino , Louis Leong , Knut Madden , Martin A. G. Gleeson , James Fan , Michael A. Brasch , David Cheo , James L. Hartley , Devon R.N. Byrd , Gary F. Temple
CPC分类号： C12N15/66 , C12N9/90 , C12N15/10 , C12N15/64 , C12N15/902 , C12N2800/108 , C12N2800/30 , C12N2800/70 , C12N2840/20 , C12P19/34 , C12Y599/01
摘要： The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according tb the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.
摘要翻译： 本发明提供了用于重组克隆的组合物和方法。 组合物包括具有多个重组位点和/或多个拓扑异构酶识别位点的载体。 该方法允许同时克隆两种或更多种不同的核酸分子。 在一些实施方案中，分子融合在一起，而在其它实施方案中，分子被插入载体中的不同位点。 本发明还通常提供通过重组连接或连接许多可以相同或不同的分子和/或化合物（例如，化合物，药物，蛋白质或肽，脂质，核酸，碳水化合物等）。 本发明还提供包含本发明的核酸分子或根据本发明的方法制备的宿主细胞，并且还提供了包含本发明的组合物，宿主细胞和核酸分子的试剂盒，其可用于合成核酸分子 根据本发明的方法。
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6.发明授权Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites 有权使用多个识别位点合成核酸分子的方法和组合物公开(公告)号：US08030066B2
公开(公告)日：2011-10-04
申请号：US11612445
申请日：2006-12-18
申请人： Jonathan D. Chesnut , John Carrino , Louis Leong , Knut Madden , Martin Gleeson , James Fan , Michael Brasch , David Cheo , James L. Hartley , Devon R. Byrd , Gray F. Temple
发明人： Jonathan D. Chesnut , John Carrino , Louis Leong , Knut Madden , Martin Gleeson , James Fan , Michael Brasch , David Cheo , James L. Hartley , Devon R. Byrd , Gray F. Temple
IPC分类号： C12N15/00
CPC分类号： C12N15/66 , C12N9/90 , C12N15/10 , C12N15/64 , C12N15/902 , C12N2800/108 , C12N2800/30 , C12N2800/70 , C12N2840/20 , C12P19/34 , C12Y599/01
摘要： The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.
摘要翻译： 本发明提供了用于重组克隆的组合物和方法。 组合物包括具有多个重组位点和/或多个拓扑异构酶识别位点的载体。 该方法允许同时克隆两种或更多种不同的核酸分子。 在一些实施方案中，分子融合在一起，而在其它实施方案中，分子被插入载体中的不同位点。 本发明还通常提供通过重组连接或连接许多可以相同或不同的分子和/或化合物（例如，化合物，药物，蛋白质或肽，脂质，核酸，碳水化合物等）。 本发明还提供了包含本发明的核酸分子或根据本发明的方法制备的宿主细胞，还提供了包含本发明的组合物，宿主细胞和核酸分子的试剂盒，其可用于合成核酸分子 根据本发明的方法。
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7.发明申请METHODS AND COMPOSITIONS FOR SYNTHESIS OF NUCLEIC ACID MOLECULES USING MULTIPLE RECOGNITION SITES 有权使用多个识别位点合成核酸分子的方法和组合物公开(公告)号：US20080241889A1
公开(公告)日：2008-10-02
申请号：US11612445
申请日：2006-12-18
申请人： Jonathan D. Chesnut , John Carrino , Louis Leong , Knut Madden , Martin A.G. Gleeson , James Fan , Michael A. Brasch , David Cheo , James L. Hartley , Devon R.N. Byrd , Gray F. Temple
发明人： Jonathan D. Chesnut , John Carrino , Louis Leong , Knut Madden , Martin A.G. Gleeson , James Fan , Michael A. Brasch , David Cheo , James L. Hartley , Devon R.N. Byrd , Gray F. Temple
CPC分类号： C12N15/66 , C12N9/90 , C12N15/10 , C12N15/64 , C12N15/902 , C12N2800/108 , C12N2800/30 , C12N2800/70 , C12N2840/20 , C12P19/34 , C12Y599/01
摘要： The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.
摘要翻译： 本发明提供了用于重组克隆的组合物和方法。 组合物包括具有多个重组位点和/或多个拓扑异构酶识别位点的载体。 该方法允许同时克隆两种或更多种不同的核酸分子。 在一些实施方案中，分子融合在一起，而在其它实施方案中，分子被插入载体中的不同位点。 本发明还通常提供通过重组连接或连接许多可以相同或不同的分子和/或化合物（例如，化合物，药物，蛋白质或肽，脂质，核酸，碳水化合物等）。 本发明还提供了包含本发明的核酸分子或根据本发明的方法制备的宿主细胞，还提供了包含本发明的组合物，宿主细胞和核酸分子的试剂盒，其可用于合成核酸分子 根据本发明的方法。
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8.发明授权Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites 有权使用多个识别位点合成核酸分子的方法和组合物公开(公告)号：US07198924B2
公开(公告)日：2007-04-03
申请号：US10792035
申请日：2004-03-04
申请人： Jonathan D. Chesnut , John Carrino , Louis Leong , Knut Madden , Martin A. G. Gleeson , James Fan , Michael A. Brasch , David Cheo , James L. Hartley , Devon R. N. Byrd , Gary F. Temple
发明人： Jonathan D. Chesnut , John Carrino , Louis Leong , Knut Madden , Martin A. G. Gleeson , James Fan , Michael A. Brasch , David Cheo , James L. Hartley , Devon R. N. Byrd , Gary F. Temple
CPC分类号： C12N15/66 , C12N9/90 , C12N15/10 , C12N15/64 , C12N15/902 , C12N2800/108 , C12N2800/30 , C12N2800/70 , C12N2840/20 , C12P19/34 , C12Y599/01
摘要： The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.
摘要翻译： 本发明提供了用于重组克隆的组合物和方法。 组合物包括具有多个重组位点和/或多个拓扑异构酶识别位点的载体。 该方法允许同时克隆两种或更多种不同的核酸分子。 在一些实施方案中，分子融合在一起，而在其它实施方案中，分子被插入载体中的不同位点。 本发明还通常提供通过重组连接或连接许多可以相同或不同的分子和/或化合物（例如，化合物，药物，蛋白质或肽，脂质，核酸，碳水化合物等）。 本发明还提供了包含本发明的核酸分子或根据本发明的方法制备的宿主细胞，还提供了包含本发明的组合物，宿主细胞和核酸分子的试剂盒，其可用于合成核酸分子 根据本发明的方法。
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9.发明申请METHODS AND COMPOSITIONS FOR DETECTING PROMOTER ACTIVITY AND EXPRESSING FUSION PROTEINS 审中-公开检测促进剂活性和表达融合蛋白的方法和组合物公开(公告)号：US20140051843A1
公开(公告)日：2014-02-20
申请号：US13540574
申请日：2012-07-02
申请人： Harry YIM , James Fan , Jonathan Chesnut , Kenneth Frimpong , Laura Vozza-Brown , Louis Leong , Peter Welch , Robert Bennett
发明人： Harry YIM , James Fan , Jonathan Chesnut , Kenneth Frimpong , Laura Vozza-Brown , Louis Leong , Peter Welch , Robert Bennett
摘要： The present invention provides nucleic acid molecules comprising one or more nucleic acid sequences encoding a polypeptide having a detectable activity. The present invention also provides methods of joining such nucleic acid molecules to nucleic acid molecules to be assayed for promoter activity. The present invention also relates to methods of preparing fusion proteins comprising a polypeptide of interest and a polypeptide having a detectable activity.
摘要翻译： 本发明提供了包含编码具有可检测活性的多肽的一种或多种核酸序列的核酸分子。 本发明还提供了将这种核酸分子连接到要测定启动子活性的核酸分子的方法。 本发明还涉及制备包含感兴趣的多肽和具有可检测活性的多肽的融合蛋白的方法。
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10.发明申请公开(公告)号：US20130236899A1
公开(公告)日：2013-09-12
申请号：US13789002
申请日：2013-03-07
IPC分类号： G01N33/543
CPC分类号： G01N33/54306 , B01L3/5023 , B01L2300/0627 , B01L2300/069 , C12Q1/6804 , C12Q1/686 , G01N33/543 , G01N33/54353 , G01N33/54366 , G01N33/553
摘要： Compositions, methods, and devices for the detection of multiple analytes with a single signal are provided.
摘要翻译： 提供了使用单个信号检测多个分析物的组合物，方法和设备。
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