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公开(公告)号:US08975471B2
公开(公告)日:2015-03-10
申请号:US11247587
申请日:2005-10-12
申请人: Jose L. Reyes Taboada , Xiuren Zhang , Takashi Soyano , Nam-Hai Chua , Qi-Wen Niu , Shih-Shun Lin
发明人: Jose L. Reyes Taboada , Xiuren Zhang , Takashi Soyano , Nam-Hai Chua , Qi-Wen Niu , Shih-Shun Lin
IPC分类号: C12N15/82 , C12N15/113 , A01H5/00
CPC分类号: C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8261 , C12N15/827 , C12N15/8283 , C12N2310/141 , Y02A40/146
摘要: The invention provides methods and compositions useful in target sequence suppression, target sequence validation and target sequence down regulation. The invention provides polynucleotide constructs useful for gene silencing or RNA down regulation, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence or to down regulate RNA.
摘要翻译: 本发明提供了可用于靶序列抑制,靶序列验证和靶序列下调的方法和组合。 本发明提供了可用于基因沉默或RNA下调的多核苷酸构建体,以及包含多核苷酸的细胞,植物和种子。 本发明还提供了使用微RNA沉默靶序列或下调RNA的方法。
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公开(公告)号:US20060130176A1
公开(公告)日:2006-06-15
申请号:US11247587
申请日:2005-10-12
申请人: Jose Reyes-Taboada , Xiuren Zhang , Takashi Soyano , Nam-Hai Chua , Qi-Wen Niu , Shih-Shun Lin
发明人: Jose Reyes-Taboada , Xiuren Zhang , Takashi Soyano , Nam-Hai Chua , Qi-Wen Niu , Shih-Shun Lin
CPC分类号: C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8261 , C12N15/827 , C12N15/8283 , C12N2310/141 , Y02A40/146
摘要: The invention provides methods and compositions useful in target sequence suppression, target sequence validation and target sequence down regulation. The invention provides polynucleotide constructs useful for gene silencing or RNA down regulation, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence or to down regulate RNA.
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公开(公告)号:US20050150014A1
公开(公告)日:2005-07-07
申请号:US10750887
申请日:2004-01-05
申请人: Ching-Hsiang Hsu , Wei-Chih Su , Shyi-Dong Yeh , Shih-Shun Lin
发明人: Ching-Hsiang Hsu , Wei-Chih Su , Shyi-Dong Yeh , Shih-Shun Lin
CPC分类号: C12N15/8258 , A61K39/35 , A61K2039/517
摘要: The present invention provides a process for producing dust mite allergen comprising the steps of: (a) constructing a vector that comprises a DNA sequence encoding the dust mite allergen operably linked to a plant-specific promoter; (b) transforming a plant cell or tissue with the vector of step (a); and (c) obtaining the dust mite allergen from the transgenic plant of step (b). A process for producing an antigenic composition and an antigenic composition are also provided.
摘要翻译: 本发明提供了一种生产尘螨变应原的方法,包括以下步骤:(a)构建载体,该载体包含编码与植物特异性启动子可操作连接的尘螨过敏原的DNA序列; (b)用步骤(a)的载体转化植物细胞或组织; 和(c)从步骤(b)的转基因植物获得尘螨变应原。 还提供了制备抗原组合物和抗原组合物的方法。
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