公开(公告)号：US08444838B2

公开(公告)日：2013-05-21

申请号：US12392851

申请日：2009-02-25

申请人： Joseph Amshey ,  Thomas Diller ,  Regina Rooney

发明人： Joseph Amshey ,  Thomas Diller ,  Regina Rooney

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： C07K1/306 ,  C30B7/00 ,  C30B29/58

摘要： The present invention provides methods, compositions, and kits for protein crystallization. The present invention involves electrophoretically focusing at least a first protein species within a matrix comprising at least 2 regions of different pH, the protein being present in amount sufficient to permit crystallization within said pH gradient.

摘要翻译： 本发明提供蛋白质结晶的方法，组合物和试剂盒。 本发明涉及将包含至少两个不同pH的区域的基质中的至少第一种蛋白物质电泳聚焦，所述蛋白质的量足以允许在所述pH梯度内结晶。

公开(公告)号：US20090035868A1

公开(公告)日：2009-02-05

申请号：US12122607

申请日：2008-05-16

申请人： Thomas Diller ,  George Hanson ,  Bethany Sutton ,  Timothy Updyke

发明人： Thomas Diller ,  George Hanson ,  Bethany Sutton ,  Timothy Updyke

IPC分类号： G01N33/68

CPC分类号： G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

摘要： The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

摘要翻译： 本发明提供用于在细胞裂解物或蛋白质混合物中标记，分离和分析蛋白质，特别是感兴趣的特定蛋白质的方法和组合物。 蛋白质用胺反应性或硫醇反应性荧光染料或胺反应性荧光试剂标记，其与位于蛋白质上的胺基团反应变为荧光。 在标记步骤之后，可以分离和分析混合物内的蛋白质。 在另一个实施方案中，加入可结合标记蛋白上的标签的标签结合荧光试剂以特异性标记所关注的蛋白质。

公开(公告)号：US08609423B2

公开(公告)日：2013-12-17

申请号：US12122607

申请日：2008-05-16

申请人： Thomas Diller ,  George Hanson ,  Bethany Sutton ,  Timothy Updyke

发明人： Thomas Diller ,  George Hanson ,  Bethany Sutton ,  Timothy Updyke

IPC分类号： G01N33/68 ,  G01N33/00

CPC分类号： G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

摘要： The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

摘要翻译： 本发明提供用于在细胞裂解物或蛋白质混合物中标记，分离和分析蛋白质，特别是感兴趣的特定蛋白质的方法和组合物。 蛋白质用胺反应性或硫醇反应性荧光染料或胺反应性荧光试剂标记，其与位于蛋白质上的胺基团反应变为荧光。 在标记步骤之后，可以分离和分析混合物内的蛋白质。 在另一个实施方案中，加入可结合标记蛋白上的标签的标签结合荧光试剂以特异性标记所关注的蛋白质。

公开(公告)号：US20120234678A1

公开(公告)日：2012-09-20

申请号：US13392493

申请日：2009-09-03

申请人： Thomas Diller ,  Timothy Updyke

发明人： Thomas Diller ,  Timothy Updyke

IPC分类号： G01N21/64 ,  G01N27/447

CPC分类号： G01N33/6803 ,  B33Y80/00 ,  C07K1/26 ,  Y10T436/10 ,  Y10T436/105831

摘要： The present invention provides dual labeled protein standards useful for the simultaneous determination of the molecular weight of a subject protein as well as the relative mass (i.e., amount) of the subject protein present in an electrophoresis lane. The invention is also directed to methods suitable for the preparation of such dual labeled protein standards and to methods of using such dual labeled proteins to simultaneously determine the molecular weight and the relative amount of a subject protein. Further embodiments are directed to the use dual labeled protein standards to make a more accurate determination of the amount of a protein present in an electrophoresis lane. Yet further embodiments are directed to kits containing the presently described dual protein standards. Dual labeled protein standards made and used in accordance with the embodiments set forth herein may be used to simultaneously determine the molecular weight and the relative amount of a subject protein in real time.

摘要翻译： 本发明提供了用于同时测定受试蛋白的分子量以及存在于电泳泳道中的受试蛋白的相对质量（即，量）的双重标记蛋白质标准。 本发明还涉及适用于制备这种双重标记的蛋白质标准品的方法以及使用这种双标记蛋白质同时测定受试蛋白质的分子量和相对量的方法。 进一步的实施方案涉及使用双重标记的蛋白质标准物来更准确地测定电泳泳道中存在的蛋白质的量。 另外的实施方案涉及包含目前描述的双重蛋白质标准品的试剂盒。 根据本文所述的实施方案制备和使用的双重标记的蛋白质标准物可用于同时确定受试蛋白质的分子量和相对量。

公开(公告)号：US08778685B2

公开(公告)日：2014-07-15

申请号：US13392493

申请日：2010-08-25

申请人： Thomas Diller ,  Timothy Updyke

发明人： Thomas Diller ,  Timothy Updyke

IPC分类号： G01N33/68 ,  G01N21/64 ,  G01N27/447

CPC分类号： G01N33/6803 ,  B33Y80/00 ,  C07K1/26 ,  Y10T436/10 ,  Y10T436/105831

摘要： The present invention provides dual labeled protein standards useful for the simultaneous determination of the molecular weight of a subject protein as well as the relative mass (i.e., amount) of the subject protein present in an electrophoresis lane. The invention is also directed to methods suitable for the preparation of such dual labeled protein standards and to methods of using such dual labeled proteins to simultaneously determine the molecular weight and the relative amount of a subject protein. Further embodiments are directed to the use dual labeled protein standards to make a more accurate determination of the amount of a protein present in an electrophoresis lane. Yet further embodiments are directed to kits containing the presently described dual protein standards. Dual labeled protein standards made and used in accordance with the embodiments set forth herein may be used to simultaneously determine the molecular weight and the relative amount of a subject protein in real time.

摘要翻译： 本发明提供了用于同时测定受试蛋白的分子量以及存在于电泳泳道中的受试蛋白的相对质量（即，量）的双重标记蛋白质标准。 本发明还涉及适用于制备这种双重标记的蛋白质标准品的方法以及使用这种双标记蛋白质同时测定受试蛋白质的分子量和相对量的方法。 进一步的实施方案涉及使用双重标记的蛋白质标准物来更准确地测定电泳泳道中存在的蛋白质的量。 另外的实施方案涉及包含目前描述的双重蛋白质标准品的试剂盒。 根据本文所述的实施方案制备和使用的双重标记的蛋白质标准物可用于同时确定受试蛋白质的分子量和相对量。