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公开(公告)号：US08444838B2
公开(公告)日：2013-05-21
申请号：US12392851
申请日：2009-02-25
申请人： Joseph Amshey , Thomas Diller , Regina Rooney
发明人： Joseph Amshey , Thomas Diller , Regina Rooney
IPC分类号： G01N27/447 , G01N27/453
摘要： The present invention provides methods, compositions, and kits for protein crystallization. The present invention involves electrophoretically focusing at least a first protein species within a matrix comprising at least 2 regions of different pH, the protein being present in amount sufficient to permit crystallization within said pH gradient.
摘要翻译： 本发明提供蛋白质结晶的方法，组合物和试剂盒。 本发明涉及将包含至少两个不同pH的区域的基质中的至少第一种蛋白物质电泳聚焦，所述蛋白质的量足以允许在所述pH梯度内结晶。
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公开(公告)号：US20070290173A1
公开(公告)日：2007-12-20
申请号：US11840164
申请日：2007-08-16
CPC分类号： C07K7/08 , C07K1/1075 , C07K1/1077 , C07K1/26 , C07K14/00 , G01N27/44726
摘要： The invention relates to marker molecules for identifying physical properties of molecular species separated by the use of electrophoretic systems. The invention further relates to methods for preparing and using marker molecules.
摘要翻译： 本发明涉及用于鉴定通过使用电泳系统分离的分子物质的物理性质的标记分子。 本发明还涉及制备和使用标记分子的方法。
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公开(公告)号：US20050023139A1
公开(公告)日：2005-02-03
申请号：US10870131
申请日：2004-06-16
申请人： Regina Rooney , Bradley Scott , Thomas Jackson , Joseph Amshey
发明人： Regina Rooney , Bradley Scott , Thomas Jackson , Joseph Amshey
IPC分类号： C02F1/469 , G01N27/26 , G01N27/447
CPC分类号： G01N27/44704 , C02F1/4696 , G01N27/44795
摘要： A multielevational cover for assembling a fluid retaining chamber on a face of an electrophoresis cassette is presented. The cover comprises a cassette-containing member, at least one chamber-defining member, and sealing means, typically sealing means that are capable of reversible attachment. Also presented are methods for contacting cassette-immobilized gels to desired fluids by assembling a fluid retaining chamber upon the cassette using the covers of the present invention.
摘要翻译： 提出了一种用于在电泳盒的表面上组装流体保持室的多电极盖。 该盖包括一个含盒的构件，至少一个室限定构件和密封装置，通常是能够可逆地附接的密封装置。 还提出了通过使用本发明的盖子将盒固定的凝胶与所需流体接触的方法，该流体保持室通过在盒上组装。
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公开(公告)号：US20050103629A1
公开(公告)日：2005-05-19
申请号：US10961308
申请日：2004-10-07
申请人： Tom Diller , Tom Beardslee , Regina Rooney , Joseph Amshey
发明人： Tom Diller , Tom Beardslee , Regina Rooney , Joseph Amshey
IPC分类号： G01N27/447 , G01N33/559
CPC分类号： G01N27/44795 , G01N27/44747
摘要： Provided herein are rapidly-rehydratable prior-cast, dehydrated, electrophoresis separation media particularly useful for isoelectric focusing, including methods of making, and methods of use thereof.
摘要翻译： 本文提供了对于等电聚焦特别有用的快速可再氢化的预铸，脱水，电泳分离介质，包括制备方法及其使用方法。
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5.发明授权公开(公告)号：US08034223B2
公开(公告)日：2011-10-11
申请号：US12485379
申请日：2009-06-16
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： C07K1/26 , G01N27/44704 , G01N27/44747 , G01N27/44795 , G01N33/559 , G01N33/561
摘要： Methods and apparatus are presented that facilitate electrophoresis of prior-cast, hydratable separation media, usefully immobilized pH gradient (IPG) strips. The method exploits the swelling of prior-cast, hydratable separation media upon rehydration to help lodge the media in an enclosing member that permits spaced electrical communication with the enclosed separation media. The electrical communication permits a voltage gradient to be established in the enclosed separation medium sufficient to effect separation of analytes therein. Cassettes, buffer cores, electrophoresis systems and kits are presented for effecting the methods of the invention.
摘要翻译： 提出了方便和装置，其促进了预先浇铸的可水合分离介质的电泳，有用的固定化pH梯度（IPG）条带。 该方法利用了预先浇铸的可水合分离介质在再水合时的溶胀，以帮助将介质置于封闭的构件中，该封闭构件允许与封闭的分离介质间隔开的电连通。 电气通信允许在封闭的分离介质中建立足以实现其中分析物分离的电压梯度。 提出了盒，缓冲核心，电泳系统和试剂盒用于实现本发明的方法。
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公开(公告)号：US20050202405A1
公开(公告)日：2005-09-15
申请号：US10996737
申请日：2004-11-24
申请人： Joseph Amshey , Tom Diller , Regina Rooney
发明人： Joseph Amshey , Tom Diller , Regina Rooney
IPC分类号： C12Q1/00 , C30B20060101
摘要： The present invention provides methods, compositions, and kits for protein crystallization. The present invention involves electrophoretically focusing at least a first protein species within a matrix comprising at least 2 regions of different pH, the protein being present in amount sufficient to permit crystallization within said pH gradient.
摘要翻译： 本发明提供蛋白质结晶的方法，组合物和试剂盒。 本发明涉及将包含至少两个不同pH的区域的基质中的至少第一种蛋白物质电泳聚焦，所述蛋白质的量足以允许在所述pH梯度内结晶。
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7.发明申请Methods and apparatus for electrophoresis of prior-cast, hydratable separation media 审中-公开预浇铸水合分离介质的电泳方法和装置公开(公告)号：US20050284764A1
公开(公告)日：2005-12-29
申请号：US11179823
申请日：2005-07-11
IPC分类号： B01D57/02 , B03C5/00 , C07K1/26 , C08F2/58 , G01N27/447 , G01N33/559 , G01N33/561 , B01D59/42
CPC分类号： G01N27/44795 , C07K1/26 , G01N27/44704 , G01N33/559 , G01N33/561
摘要： Methods and apparatus are presented that facilitate electrophoresis of prior-cast, hydratable separation media, usefully immobilized pH gradient (IPG) strips. The method exploits the swelling of prior-cast, hydratable separation media upon rehydration to help lodge the media in an enclosing member that permits spaced electrical communication with the enclosed separation media. The electrical communication permits a voltage gradient to be established in the enclosed separation medium sufficient to effect separation of analytes therein. Cassettes, buffer cores, electrophoresis systems and kits are presented for effecting the methods of the invention.
摘要翻译： 提出了方便和装置，其促进了预先浇铸的可水合分离介质的电泳，有用的固定化pH梯度（IPG）条带。 该方法利用了预先浇铸的可水合分离介质在再水合时的溶胀，以帮助将介质置于封闭的构件中，该封闭构件允许与封闭的分离介质间隔开的电连通。 电气通信允许在封闭的分离介质中建立足以实现其中分析物分离的电压梯度。 提出了盒，缓冲核心，电泳系统和试剂盒用于实现本发明的方法。
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公开(公告)号：US08039264B2
公开(公告)日：2011-10-18
申请号：US12510995
申请日：2009-07-28
申请人： Roumen Bogoev , Joseph Amshey , George Hanson
发明人： Roumen Bogoev , Joseph Amshey , George Hanson
IPC分类号： G01N33/68
CPC分类号： C12Y302/01031 , C07K1/13 , C12N9/1033 , C12N9/2442 , C12Y302/01014 , G01N33/533 , Y10T436/105831 , Y10T436/13 , Y10T436/18 , Y10T436/25
摘要： Methods, compositions, and kits for labeling tetracysteine-tagged proteins with biarsenical fluorophores with increased specificity, including compositions, methods and kits particularly adapted for labeling of tetracysteine-tagged proteins to be resolved within an electrophoresis gel.
摘要翻译： 用于用具有增加的特异性的双缩氟荧光团标记四半胱氨酸标记的蛋白质的方法，组合物和试剂盒，包括组合物，方法和试剂盒，特别适用于在电泳凝胶内标记待分解的四半胱氨酸标记的蛋白质。
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9.发明申请公开(公告)号：US20050239135A1
公开(公告)日：2005-10-27
申请号：US10971606
申请日：2004-10-22
申请人： Roumen Bogoev , Joseph Amshey , George Hanson
发明人： Roumen Bogoev , Joseph Amshey , George Hanson
CPC分类号： C12Y302/01031 , C07K1/13 , C12N9/1033 , C12N9/2442 , C12Y302/01014 , G01N33/533 , Y10T436/105831 , Y10T436/13 , Y10T436/18 , Y10T436/25
摘要： Methods, compositions, and kits for labeling tetracysteine-tagged proteins with biarsenical fluorophores with increased specificity, including compositions, methods and kits particularly adapted for labeling of tetracysteine-tagged proteins to be resolved within an electrophoresis gel.
摘要翻译： 用于用具有增加的特异性的双缩氟荧光团标记四半胱氨酸标记的蛋白质的方法，组合物和试剂盒，包括组合物，方法和试剂盒，特别适用于在电泳凝胶内标记待分解的四半胱氨酸标记的蛋白质。
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公开(公告)号：US08404198B2
公开(公告)日：2013-03-26
申请号：US12549311
申请日：2009-08-27
申请人： Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Korneija Zgonc
发明人： Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Korneija Zgonc
IPC分类号： B01L9/00
CPC分类号： B01L3/502715 , B01L3/50273 , B01L7/00 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/10 , B01L2200/16 , B01L2300/0636 , B01L2300/0681 , B01L2300/0867 , B01L2300/088 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , B01L2400/0655 , G01N2035/00306 , G01N2035/1037 , Y10T436/2575
摘要： The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.
摘要翻译： 本发明提供了用于自动化生物处理的系统，装置，装置和方法。 适用于本发明的方案和生物处理方法的实例包括但不限于：免疫沉淀，染色质免疫沉淀，重组蛋白分离，核酸分离和分离，蛋白标记，分离和分离，细胞分离和分离，食品安全分析和自动 珠基分离。 在一些实施方案中，本发明提供自动化系统，自动化装置，自动化盒和western印迹处理的自动化方法。 其他实施方案包括自动化系统，自动化装置，自动化盒和用于分离，制备和纯化核酸例如DNA或RNA或其片段的自动化方法，包括质粒DNA，基因组DNA，细菌DNA，病毒DNA和任何其它DNA， 以及用于自动化系统，自动化设备，自动化盒和用于处理，分离和纯化蛋白质，肽等的自动化盒和自动化方法。
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