Process for the sterile micropropagation of plant material
    2.
    发明授权
    Process for the sterile micropropagation of plant material 失效
    植物材料的无菌微繁殖方法

    公开(公告)号:US4569914A

    公开(公告)日:1986-02-11

    申请号:US508613

    申请日:1983-06-28

    IPC分类号: A01H3/00 A01H4/00 C12N5/00

    CPC分类号: A01H4/005 Y10S47/03

    摘要: The invention relates to a process for the production of propagating material of plants by micropropagation in sterile tissue culture which comprises(a) planting sterile shoot cuttings of plants in solid nutrient medium in a suitable flask, filling up the flask with a suitable liquid nutrient medium optionally containing a growth regulator and growing the shoots in the submersed system; thereafter(b) cutting up into pieces the ramified culture thus obtained which contains a number of lateral buds and shoots, rooting the top-end shoots and planting the same after adaptation, and subjecting the cuttings prepared from the lower and middle parts of the shoot cultures to further cultivations according to step (a);and repeating steps (a) and (b) until the desired number of top-end shoots is reached.

    摘要翻译: 本发明涉及通过在无菌组织培养物中的微繁殖来生产植物繁殖材料的方法,其包括(a)在合适的烧瓶中将固体营养培养基中的植物的无菌枝条种植,用合适的液体营养培养基填充烧瓶 任选地含有生长调节剂并在浸没体系中生长芽; 此后(b)将如此获得的分枝培养物切成片,其中包含许多侧芽和芽,在适应后生根顶端芽并种植它们,并对从枝条的下部和中部制备的切片 文化进一步根据步骤(a)进行培养; 并重复步骤(a)和(b),直到达到所需数量的顶端射击。