公开(公告)号：US08110381B2

公开(公告)日：2012-02-07

申请号：US12034087

申请日：2008-02-20

申请人： Seiko Hirano ,  Jun Nakamura ,  Hisao Ito

发明人： Seiko Hirano ,  Jun Nakamura ,  Hisao Ito

IPC分类号： C12P13/14

CPC分类号： C12P13/14

摘要： A method for producing L-glutamic acid by culturing a coryneform bacterium in which the gluX is inactivated in a medium to produce L-glutamic acid in the medium or cells, and collecting L-glutamic acid from the medium.

摘要翻译： 通过培养在培养基中使gluX失活以产生L-谷氨酸的棒状细菌并从培养基中收集L-谷氨酸的棒状细菌的生产方法。

公开(公告)号：US07794989B2

公开(公告)日：2010-09-14

申请号：US11275346

申请日：2005-12-28

申请人： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

发明人： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12N1/21

CPC分类号： C12P13/14 ,  C07K14/34

摘要： A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells.

摘要翻译： 通过在培养基中培养通过使用yggB基因修饰的棒状细菌，使得与非修饰菌株相比增强L-谷氨酸生产能力，以在培养基或细菌细胞中引起L-谷氨酸的积累， 从培养基或细胞收集L-谷氨酸。

公开(公告)号：US08278074B2

公开(公告)日：2012-10-02

申请号：US13047260

申请日：2011-03-14

申请人： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

发明人： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12N1/20

CPC分类号： C12P13/14 ,  C07K14/34

摘要： A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells.

摘要翻译： 通过在培养基中培养通过使用yggB基因修饰的棒状细菌，使得与非修饰菌株相比增强L-谷氨酸生产能力，以在培养基或细菌细胞中引起L-谷氨酸的积累， 从培养基或细胞收集L-谷氨酸。

公开(公告)号：US07867735B2

公开(公告)日：2011-01-11

申请号：US12034101

申请日：2008-02-20

申请人： Seiko Hirano ,  Jun Nakamura ,  Hisao Ito

发明人： Seiko Hirano ,  Jun Nakamura ,  Hisao Ito

IPC分类号： C12P21/00 ,  C12N1/20

CPC分类号： C12P13/14 ,  C07K14/34

摘要： A method for producing L-glutamic acid by culturing a coryneform bacterium which has L-glutamic acid producing ability and which has been modified so that expression of the fasR gene is enhanced in a medium to produce and accumulate L-glutamic acid in the medium or cells, and collecting L-glutamic acid from the medium or cells.

摘要翻译： 通过培养具有L-谷氨酸生产能力的棒状细菌生产L-谷氨酸的方法，其已被修饰以使培养基中的fasR基因的表达增强以产生和积累L-谷氨酸，或者 细胞，并从培养基或细胞中收集L-谷氨酸。

公开(公告)号：US20060141588A1

公开(公告)日：2006-06-29

申请号：US11275346

申请日：2005-12-28

申请人： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

发明人： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12N1/21

CPC分类号： C12P13/14 ,  C07K14/34

摘要： A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells.

摘要翻译： 通过在培养基中培养通过使用yggB基因修饰的棒状细菌，使得与非修饰菌株相比增强L-谷氨酸生产能力，以在培养基或细菌细胞中引起L-谷氨酸的积累， 从培养基或细胞收集L-谷氨酸。

公开(公告)号：US07927844B2

公开(公告)日：2011-04-19

申请号：US12550929

申请日：2009-08-31

申请人： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

发明人： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12N1/21

CPC分类号： C12P13/14 ,  C07K14/34

摘要： A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells.

摘要翻译： 通过在培养基中培养通过使用yggB基因修饰的棒状细菌，使得与非修饰菌株相比增强L-谷氨酸生产能力，以在培养基或细菌细胞中引起L-谷氨酸的积累， 从培养基或细胞收集L-谷氨酸。

公开(公告)号：US20110171679A1

公开(公告)日：2011-07-14

申请号：US13047260

申请日：2011-03-14

申请人： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

发明人： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

IPC分类号： C12Q1/02

CPC分类号： C12P13/14 ,  C07K14/34

摘要： A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells.

摘要翻译： 通过在培养基中培养通过使用yggB基因修饰的棒状细菌，使得与非修饰菌株相比增强L-谷氨酸生产能力，以在培养基或细菌细胞中引起L-谷氨酸的积累， 从培养基或细胞收集L-谷氨酸。

公开(公告)号：US20090258402A1

公开(公告)日：2009-10-15

申请号：US12034101

申请日：2008-02-20

申请人： Seiko Hirano ,  Jun Nakamura ,  Hisao Ito

发明人： Seiko Hirano ,  Jun Nakamura ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12N1/20

CPC分类号： C12P13/14 ,  C07K14/34

摘要： A method for producing L-glutamic acid by culturing a coryneform bacterium which has L-glutamic acid producing ability and which has been modified so that expression of the fasR gene is enhanced in a medium to produce and accumulate L-glutamic acid in the medium or cells, and collecting L-glutamic acid from the medium or cells.

摘要翻译： 通过培养具有L-谷氨酸生产能力的棒状细菌生产L-谷氨酸的方法，其已被修饰以使培养基中的fasR基因的表达增强以产生和积累L-谷氨酸，或者 细胞，并从培养基或细胞中收集L-谷氨酸。

公开(公告)号：US20070172932A1

公开(公告)日：2007-07-26

申请号：US11682370

申请日：2007-03-06

申请人： Seiko Hirano ,  Mikiko Yamaguchi ,  Jun Nakamura ,  Hisao Ito

发明人： Seiko Hirano ,  Mikiko Yamaguchi ,  Jun Nakamura ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12P13/14 ,  C12N9/0012

摘要： A coryneform bacterium which has an L-glutamic acid-producing ability and grows at least at the same growth rate as a non-mutated strain or a wild-type strain and has intracellular α-ketoglutarate dehydrogenase activity which is less than half that of the non-mutated or wild-type strain, and is obtained by introducing a mutation into a coding region or an expression control region of the chromosomal odhA gene encoding the E1o subunit of the α-ketoglutarate dehydrogenase complex.

摘要翻译： 具有L-谷氨酸生产能力的棒状杆菌型细菌至少以与非突变菌株或野生型菌株相同的生长速率生长，并且具有小于所述野生型菌株的一半的细胞内α-酮戊二酸脱氢酶活性 非突变或野生型菌株，并且通过将突变引入编码α-酮戊二酸脱氢酶复合物的E1o亚基的染色体odhA基因的编码区域或表达控制区而获得。

公开(公告)号：US07205132B2

公开(公告)日：2007-04-17

申请号：US11222138

申请日：2005-09-09

申请人： Seiko Hirano ,  Mikiko Yamaguchi ,  Jun Nakamura ,  Hisao Ito

发明人： Seiko Hirano ,  Mikiko Yamaguchi ,  Jun Nakamura ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12Q1/04 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12P13/14 ,  C12N9/0012

摘要： A coryneform bacterium which has an L-glutamic acid-producing ability and grows at least at the same growth rate as a non-mutated strain or a wild-type strain and has intracellular α-ketoglutarate dehydrogenase activity which is less than half that of the non-mutated or wild-type strain, and is obtained by introducing a mutation into a coding region or an expression control region of the chromosomal odhA gene encoding the E1o subunit of the α-ketoglutarate dehydrogenase complex.

摘要翻译： 具有L-谷氨酸生产能力的棒状杆菌型细菌至少以与非突变菌株或野生型菌株相同的生长速率生长，并且具有小于所述野生型菌株的一半的细胞内α-酮戊二酸脱氢酶活性 非突变或野生型菌株，并且通过将突变引入编码α-酮戊二酸脱氢酶复合物的E1o亚基的染色体odhA基因的编码区域或表达控制区而获得。