公开(公告)号：US5856134A

公开(公告)日：1999-01-05

申请号：US461361

申请日：1995-06-05

申请人： Jungsuh P. Kim ,  Kirk E. Fry ,  LaVonne Marie Young ,  Jeffrey M. Linnen ,  John Wages

发明人： Jungsuh P. Kim ,  Kirk E. Fry ,  LaVonne Marie Young ,  Jeffrey M. Linnen ,  John Wages

IPC分类号： A61K38/00 ,  C07K14/18 ,  C12N1/19 ,  C12N1/21 ,  C12P21/08 ,  C12P21/02 ,  A61K39/29

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N2770/24222 ,  C12N2770/36022 ,  Y10S530/826

摘要： Polypeptide antigens are disclosed which are immunoreactive with sera from individuals having a non-A, non-B, non-C, non-D, non-E Hepatitis, herein designated Hepatitis G Virus (HGV). Corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic polypeptides are taught. The antigens are useful in diagnostic methods for detecting the presence of HGV in test subjects. The antigens are also useful in vaccine and antibody preparations. In addition, the entire coding sequences of two HGV isolates are disclosed. Methods are presented for nucleic acid-based detection of HGV in samples and also methods for the isolation of further genomic sequences corresponding to HGV.

公开(公告)号：US5824507A

公开(公告)日：1998-10-20

申请号：US444733

申请日：1995-05-19

申请人： Jungsuh P. Kim ,  Kirk E. Fry ,  LaVonne Marie Young ,  Jeffrey M. Linnen ,  John Wages

发明人： Jungsuh P. Kim ,  Kirk E. Fry ,  LaVonne Marie Young ,  Jeffrey M. Linnen ,  John Wages

IPC分类号： A61K38/00 ,  C07K14/18 ,  C12N1/19 ,  C12N1/21 ,  C12P21/08 ,  C12Q1/70 ,  C12N15/03

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N2770/24222 ,  C12N2770/36022 ,  Y10S530/826

摘要： Polypeptide antigens are disclosed which are immunoreactive with sera from individuals having a non-A, non-B, non-C, non-D, non-E Hepatitis, herein designated Hepatitis G Virus (HGV). Corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic polypeptides are taught. The antigens are useful in diagnostic methods for detecting the presence of HGV in test subjects. The antigens are also useful in vaccine and antibody preparations. In addition, the entire coding sequences of two HGV isolates are disclosed. Methods are presented for nucleic acid-based detection of HGV in samples and also methods for the isolation of further genomic sequences corresponding to HGV.

公开(公告)号：US5849532A

公开(公告)日：1998-12-15

申请号：US464134

申请日：1995-06-06

申请人： Jungsuh P. Kim ,  Kirk E. Fry ,  LaVonne Marie Young ,  Jeffrey M. Linnen ,  John Wages

发明人： Jungsuh P. Kim ,  Kirk E. Fry ,  LaVonne Marie Young ,  Jeffrey M. Linnen ,  John Wages

IPC分类号： A61K38/00 ,  C07K14/18 ,  C12N1/19 ,  C12N1/21 ,  C12P21/08

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N2770/24222 ,  C12N2770/36022 ,  Y10S530/826

摘要： Polypeptide antigens are disclosed which are immunoreactive with sera from individuals having a non-A, non-B, non-C, non-D, non-E Hepatitis, herein designated Hepatitis G Virus (HGV). Corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic polypeptides are taught. The antigens are useful in diagnostic methods for detecting the presence of HGV in test subjects. The antigens are also useful in vaccine and antibody preparations. In addition, the entire coding sequences of two HGV isolates are disclosed. Methods are presented for nucleic acid-based detection of HGV in samples and also methods for the isolation of further genomic sequences corresponding to HGV.

公开(公告)号：US5766840A

公开(公告)日：1998-06-16

申请号：US466033

申请日：1995-06-05

申请人： Jungsuh P. Kim ,  Kirk E. Fry ,  LaVonne Marie Young ,  Jeffrey M. Linnen ,  John Wages

发明人： Jungsuh P. Kim ,  Kirk E. Fry ,  LaVonne Marie Young ,  Jeffrey M. Linnen ,  John Wages

IPC分类号： A61K38/00 ,  C07K14/18 ,  C12N1/19 ,  C12N1/21 ,  C12P21/08 ,  C12Q1/70 ,  C07K16/10

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N2770/24222 ,  C12N2770/36022 ,  Y10S530/826

摘要： Polypeptide antigens are disclosed which are immunoreactive with sera from individuals having a non-A, non-B, non-C, non-D, non-E Hepatitis, herein designated Hepatitis G Virus (HGV). Corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic polypeptides are taught. The antigens are useful in diagnostic methods for detecting the presence of HGV in test subjects. The antigens are also useful in vaccine and antibody preparations. In addition, the entire coding sequences of two HGV isolates are disclosed. Methods are presented for nucleic acid-based detection of HGV in samples and also methods for the isolation of further genomic sequences corresponding to HGV.

公开(公告)号：US5859230A

公开(公告)日：1999-01-12

申请号：US611757

申请日：1996-03-06

申请人： Jungsuh P. Kim ,  Gregory R. Reyes ,  LaVonne Marie Young

发明人： Jungsuh P. Kim ,  Gregory R. Reyes ,  LaVonne Marie Young

IPC分类号： A61K38/00 ,  C07K14/18 ,  C07H21/04

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N2770/24222 ,  C12N2770/34022

摘要： Polypeptide antigens are disclosed which are immunoreactive with sera from individuals infected with non-A, non-B, non-C, non-D, non-E hepatitis. Also disclosed are corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic polypeptides. The antigens are useful in diagnostic methods for detecting the presence of N-(ABCDE) hepatitis agent infection in primates. The antigens are also useful in vaccine and antibody preparations. Methods are presented for the isolation of entire genomes corresponding to the N-(ABCDE) hepatitis agents.

摘要翻译： 公开了多肽抗原，其与来自感染非A，非B，非C，非D，非E型肝炎的个体的血清具有免疫反应性。 还公开了含有编码抗原性多肽的开放阅读框序列的多核苷酸的相应的基因组片段克隆。 抗原可用于检测灵长类动物中N-（ABCDE）肝炎病毒感染的诊断方法。 抗原也可用于疫苗和抗体制剂。 提出了分离相应于N-（ABCDE）肝炎试剂的整个基因组的方法。