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公开(公告)号：US10822384B2
公开(公告)日：2020-11-03
申请号：US14389016
申请日：2013-03-29
IPC分类号： C07K17/00 , G01N33/543 , C07K14/47 , G01N33/574 , G01N33/68 , C07K1/113
摘要： The present invention provides the following: a method for efficiently producing a reagent for detecting an antibody that specifically binds with an insoluble antigen protein present in a liquid sample; a reagent for antibody detection produced by the production method; and a use of the antibody. In a step for solubilizing an antigen protein, it is possible to efficiently solubilize and recover the antigen protein by using a cationizing agent; therefore, when compared to conventional methods, it is possible to efficiently produce a reagent for detecting an antibody that has bound to multiple antigen protein molecules in a carrier.
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2.发明申请公开(公告)号：US20150064801A1
公开(公告)日：2015-03-05
申请号：US14389016
申请日：2013-03-29
IPC分类号： C07K14/47 , G01N33/574
CPC分类号： C07K14/4748 , C07K1/113 , C07K14/4703 , G01N33/543 , G01N33/574 , G01N33/57426 , G01N33/57492 , G01N33/6854 , G01N2333/47 , G01N2333/4704
摘要： The present invention provides the following: a method for efficiently producing a reagent for detecting an antibody that specifically binds with an insoluble antigen protein present in a liquid sample; a reagent for antibody detection produced by the production method; and a use of the antibody. In a step for solubilizing an antigen protein, it is possible to efficiently solubilize and recover the antigen protein by using a cationizing agent; therefore, when compared to conventional methods, it is possible to efficiently produce a reagent for detecting an antibody that has bound to multiple antigen protein molecules in a carrier.
摘要翻译： 本发明提供以下方法：一种用于有效地制备用于检测与存在于液体样品中的不溶性抗原蛋白特异性结合的抗体的试剂的方法; 通过该制造方法生产的抗体检测试剂; 和抗体的用途。 在溶解抗原蛋白的步骤中，可以通过使用阳离子化剂有效地溶解并回收抗原蛋白质; 因此，与常规方法相比，可以有效地制备用于检测在载体中与多个抗原蛋白质分子结合的抗体的试剂。
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