公开(公告)号：US20160215026A1

公开(公告)日：2016-07-28

申请号：US14916263

申请日：2014-09-05

Applicant: KANEKA CORPORATION

Inventor： Shinichi YOSHIDA ,  Kanji OSHIMA ,  Kazunobu MINAKUCHI

IPC: C07K14/31 ,  C07K16/00 ,  C07K1/22

CPC classification number: C07K14/31 ,  C07K1/22 ,  C07K16/00

Abstract: The present invention produces affinity ligands that make it possible to increase the purity of antibodies themselves, which is the main purpose of affinity purification, and, further, to improve the selectivity for antibody monomers and thereby reduce the load of removing antibody multimers on downstream purification steps. The present invention provides new affinity ligands which are modified proteins obtained by introducing a mutation into a protein to increase the number of acidic amino acids in the amino acid sequence of the protein; methods for designing the modified proteins; methods for producing the modified proteins; and affinity separation matrices containing the modified proteins as ligands immobilized therein.

Abstract translation: 本发明产生亲和配体，其可以提高抗体本身的纯度，这是亲和纯化的主要目的，此外，为了提高抗体单体的选择性，从而降低了下游纯化中除去抗体多聚体的负荷 脚步。 本发明提供了新的亲和配体，其是通过向蛋白质中引入突变而增加蛋白质的氨基酸序列中酸性氨基酸数目而获得的修饰蛋白质; 设计修饰蛋白的方法 生产修饰蛋白的方法; 和含有修饰蛋白质的亲和力分离基质作为其中固定的配体。