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1.发明公开公开(公告)号：US20230192781A1
公开(公告)日：2023-06-22
申请号：US16766845
申请日：2017-12-13
Inventor： Min GUO , Lingxuan JIANG , Zhuxia ZHENG , Xue YU
CPC classification number: C07K14/39 , C12N15/67 , C12N15/815
Abstract: What provided is a method for regulating in vitro biosynthesis activity by knocking-out of a nuclease system, comprising: screening five nucleases among numerous nucleases, and performing down-regulation or knocking-out on one of the five nucleases (e.g., EXN53). The method can improve the stability of nucleic acid and the protein production efficiency of an in vitro protein synthesis system.
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