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公开(公告)号:US20100167397A1
公开(公告)日:2010-07-01
申请号:US12562320
申请日:2009-09-18
申请人: KATSUTOSHI SASAKI , KAZUMI MIURA , SATOSHI SAEKI , MISAKO SUZUKI , KAZUYA KISHIMOTO , HIROFUMI KUNITOMO , TATSUNARI NISHI , MASUO OBINATA
发明人: KATSUTOSHI SASAKI , KAZUMI MIURA , SATOSHI SAEKI , MISAKO SUZUKI , KAZUYA KISHIMOTO , HIROFUMI KUNITOMO , TATSUNARI NISHI , MASUO OBINATA
IPC分类号: C12N5/10
CPC分类号: G01N33/507 , C12N2510/04 , C12N2830/002 , G01N33/5008 , G01N33/5023 , G01N33/5026 , G01N33/5044 , G01N33/74 , G01N2500/10
摘要: In accordance with the present invention, there are provided (1) various cell lines derived from hypothalamus and Langerhans islets of mammals, (2) process for producing an active peptide and expression cloning system of active peptide precursor gene using the cell line as a host, (3) a method of screening or evaluating a substance capable of acting on the cells using the cell line, (4) a method of screening or isolating a useful gene or useful peptide using the cell line and (5) a highly-sensitive and simple assay system for GPCR ligand used in the above expression cloning system.
摘要翻译: 根据本发明,提供了(1)衍生自哺乳动物的下丘脑和朗格汉斯胰岛的各种细胞系,(2)使用细胞系作为宿主的活性肽的生产方法和活性肽前体基因的表达克隆系统 (3)使用细胞系筛选或评价能够作用于细胞的物质的方法,(4)使用细胞系筛选或分离有用基因或有用肽的方法,和(5)高度敏感的 和用于上述表达克隆系统的GPCR配体的简单测定系统。