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    SPFS BIOSENSOR BASED ON NUCLEIC ACID LIGAND STRUCTURAL CHANGE
    3.
    发明申请
    SPFS BIOSENSOR BASED ON NUCLEIC ACID LIGAND STRUCTURAL CHANGE 审中-公开

    公开(公告)号：US20170241993A1

    公开(公告)日：2017-08-24

    申请号：US15504004

    申请日：2015-09-01

    Applicant: KONICA MINOLTA LABORATORY U.S.A., INC.

    Inventor： Noriaki YAMAMOTO

    IPC: G01N33/542 ,  G01N33/543 ,  C12Q1/68 ,  G01N21/64

    CPC classification number: G01N33/542 ,  C12Q1/6804 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/54373 ,  G01N2021/6417 ,  G01N2021/6439

    Abstract: A DNA ligand capable of structural changes upon binding to a target is used as a molecular switch with a SPFS (surface plasmon field-enhanced fluorescence spectroscopy) biosensor to realize one-step SPFS biosensing with rapid turnaround time. The SPFS biosensor has a thin metal film on a prism; when a light of a certain wavelength irradiates on the prism at a certain angle, a strong electrical field is generated at the surface of the metal film. The DNA is immobilized on the metal film surface with its free terminal modified with a fluorescent marker. Without the target, the DNA is folded and the fluorescent marker is located in the region of metal quenching near the metal surface. Upon binding to the target, the DNA is extended and the fluorescent marker is located in the region of enhanced electric field near the metal surface and emits a strong fluorescent signal.

    BIOSENSOR HAVING DECOUPLED CAPTURE CHAMBER AND DETECTION CHAMBER, USING PARTICLE AGGREGATION AND SIZE-SEPARATION
    4.
    发明申请
    BIOSENSOR HAVING DECOUPLED CAPTURE CHAMBER AND DETECTION CHAMBER, USING PARTICLE AGGREGATION AND SIZE-SEPARATION 有权
    生物传感器具有分离式捕获室和检测室，使用颗粒聚集和尺寸分离

    公开(公告)号：US20150362485A1

    公开(公告)日：2015-12-17

    申请号：US14737195

    申请日：2015-06-11

    Applicant: KONICA MINOLTA LABORATORY U.S.A., INC.

    Inventor： Noriaki YAMAMOTO

    IPC: G01N33/537

    CPC classification number: G01N33/54366 ,  B01L3/502738 ,  B01L3/502753 ,  B01L2200/0631 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/088 ,  G01N33/54313

    Abstract: A biosensor using a decoupled microfluidic device, which has a capture chamber and a detection chamber separate from and in fluid communication with each other. The sensing method is based on particle aggregation via homogeneous reactions, by employing microparticles having antibodies on their surfaces which can form aggregates through antigen mediation. Either size-separation or magnetic based separation is used to separate aggregates from single microparticles; the aggregates are later dissociated and the resulting single microparticles are counted to measure the amount of the antigen. Another biosensor uses a decoupled microfluidic device with a capture chamber and a detection chamber, and a 3-D structure in the capture camber to increase immobilized antibody concentration. Immunoreaction efficiency is improved by increasing the number of antibody per reaction volume in the capture chamber.

    Abstract translation: 一种使用解耦微流体装置的生物传感器，其具有与彼此分离和流体连通的捕获室和检测室。 感测方法基于通过均相反应的颗粒聚集，通过使用在其表面上具有抗体的微粒，其可以通过抗原调解形成聚集体。 使用尺寸分离或磁性分离来从单个微粒分离聚集体; 聚集物随后解离，并将所得单个微粒计数以测量抗原的量。 另一种生物传感器使用具有捕获室和检测室的去耦微流体装置，以及捕获场中的3-D结构以增加固定的抗体浓度。 通过增加捕获室中每反应体积的抗体数量来提高免疫反应效率。

    ULTRA-RAPID AND SENSITIVE DNA DETECTION USING DNAZYME AND ON-CHIP ISOTACHOPHORESIS
    5.
    发明申请
    ULTRA-RAPID AND SENSITIVE DNA DETECTION USING DNAZYME AND ON-CHIP ISOTACHOPHORESIS 审中-公开
    使用DNAZYME和片上异黄酮的超快速和敏感DNA检测

    公开(公告)号：US20150197791A1

    公开(公告)日：2015-07-16

    申请号：US14590482

    申请日：2015-01-06

    Applicant: KONICA MINOLTA LABORATORY U.S.A., INC.

    Inventor： Noriaki YAMAMOTO

    IPC: C12Q1/68

    CPC classification number: C12Q1/6816 ,  C12Q2521/345 ,  C12Q2525/301 ,  C12Q2565/629

    Abstract: A DNA detection method combines DNAzyme reactions and on-chip isotachophoresis (ITP). A mixture of sample containing a target DNA and a DNAzyme sensor which is either (1) a catalytic molecular beacon or (2) a binary DNAzyme and a probe is loaded into a trailing electrolyte (TE) reservoir of a microfluidic chip. In the presence of the target DNA, the catalytic molecular beacon or the probe is cleaved to generate a fluorescent fragment. Enhanced DNAzyme reaction occurs at the TE-to-LE interface. Fluorescent signal from cleaved catalytic molecular beacon or probe is detected either at the location where DNAzyme reaction occurs or at a separate location. In the latter case, the microfluidic chip has a separation region containing a capture gel or a sieving matrix which allows the fluorescent fragment to pass through but captures or traps the uncleaved catalytic molecular beacon or probe.

    Abstract translation: DNA检测方法结合DNA酶反应和片上等速电泳（ITP）。 将（1）催化分子信标或（2）二元DNA酶和探针的含有目标DNA和DNA酶传感器的样品的混合物加载到微流控芯片的尾电解质（TE）储存器中。 在靶DNA的存在下，催化分子信标或探针被切割以产生荧光片段。 增强的DNA酶反应发生在TE-to-LE界面。 在DNAzyme反应发生的地点或分开的位置检测到来自裂解的催化分子信标或探针的荧光信号。 在后一种情况下，微流体芯片具有含有捕获凝胶或筛分基质的分离区域，其允许荧光片段通过，但捕获或捕获未切割的催化分子信标或探针。

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