公开(公告)号：US20130078673A1

公开(公告)日：2013-03-28

申请号：US13667612

申请日：2012-11-02

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE A

Inventor： Sang Yup LEE ,  Jeong Wook LEE ,  Hyohak SONG ,  Ji Mahn KIM ,  Sol CHOI ,  Jin Hwan PARK

IPC: C12N15/70

CPC classification number: C12N15/70 ,  C12N1/20 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/2408 ,  C12P1/04 ,  C12P7/065 ,  C12P7/40 ,  C12P7/46 ,  C12P7/54 ,  C12P7/56 ,  C12P13/08 ,  C12Y207/01004 ,  C12Y207/01069 ,  C12Y302/01026 ,  Y02E50/17

Abstract: The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.

Abstract translation: 本发明涉及能够代谢蔗糖的重组微生物，更具体地说，涉及能够代谢蔗糖的重组微生物，其中导入编码蔗糖磷酸转移酶和/或编码蔗糖-6-磷酸水解酶的基因的基因或重组微生物 能够代谢其中引入编码“呋喃果糖苷酶”的基因的蔗糖。 根据本发明，提供了能够使用廉价的蔗糖作为碳源代替昂贵的葡萄糖的重组微生物。 此外，在不能使用蔗糖作为碳源的微生物的培养方法中，蔗糖可以替代包括葡萄糖在内的其它碳源。