公开(公告)号：US20250027049A1

公开(公告)日：2025-01-23

申请号：US18715678

申请日：2022-11-29

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Yong Mahn Han ,  Wondo Heo ,  Ji Eun Choi ,  Jinsu Lee ,  Eun Ji Shin

IPC: C12N5/071 ,  A61K35/39 ,  A61P3/10

Abstract: The present invention relates to an islet-like organoid in which insulin secretion can be optically regulated by differentiating, into pancreatic islets, human pluripotent stem cells (monSTIM1-hPSC) obtained by knocking-in monSTIM1, which is an inducer of Ca2+ concentration increase (Ca2+ transient), into the AAVS1 locus of the stem cells. Since it has been confirmed in vitro and in the body of a diabetic mouse model that the islet-like organoid of the present invention can secrete insulin through an intracellular calcium influx increased by light irradiation, the islet-like organoid can be effectively used in the treatment of diabetic patients.