公开(公告)号：US20140205998A1

公开(公告)日：2014-07-24

申请号：US13758357

申请日：2013-02-04

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Eun Joo SONG ,  Eunmi BAN ,  Dong-Kyu CHAE

IPC: C12Q1/68

CPC classification number: C12N15/1003 ,  C12Q1/6816 ,  C12Q2525/207 ,  C12Q2527/125

Abstract: The present invention relates to a method for improving detection efficiency of miRNAs existing in cells in trace amounts by adding Triton X-100. In accordance with the present invention, miRNAs existing in a sample in trace amounts can be quantitatively analyzed in short time. Further, the miRNA detection method according to the present invention wherein Triton X-100 is used can improve detection efficiency by about 2 times as compared to when only a TRIzol reagent is used.

Abstract translation: 本发明涉及通过加入Triton X-100来提高存在于微量细胞中的miRNA的检测效率的方法。 根据本发明，可以在短时间内定量分析存在于痕量样品中的miRNA。 此外，与仅使用TRIzol试剂相比，使用Triton X-100的根据本发明的miRNA检测方法可以将检测效率提高约2倍。