公开(公告)号：US20230314292A1

公开(公告)日：2023-10-05

申请号：US18078328

申请日：2022-12-09

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Man Ho CHOI ,  Jong Sung NOH ,  Seuk Min RYU

IPC: G01N1/38 ,  G01N33/74 ,  C01G9/06 ,  C01D1/04 ,  C01D7/00

CPC classification number: G01N1/38 ,  G01N33/743 ,  C01G9/06 ,  C01D1/04 ,  C01D7/00

Abstract: The present invention relates to a diluted solution for measuring free steroids in blood and a method for measuring free steroids using the same, and more particularly, to a method for preparing a diluted blood sample for measuring free steroids in blood and a composition for blood sample dilution solution.
In the method for preparing a diluted blood sample for measuring free steroids in blood of the present invention, it was confirmed that physiologically active free cortisol could be evaluated with the cortisol enzyme immunoreaction detection kit without additional sample pretreatment by removing cortisol which is present by binding to excess albumin and cortisol-specific corticosteroid-binding globulin (CBG) contained in serum using a protein precipitation and neutralizing agent. In addition, by selectively removing the protein in the blood sample, it is possible to measure low molecular weight compounds, including steroids, which are strongly bound to proteins in the blood, such as cortisol, in free form.
Therefore, the method of the present invention can be applied simply and effectively to medical sites requiring blood tests for disease diagnosis, monitoring of free cortisol before and after surgery, and emergency patients.