公开(公告)号：US20210395695A1

公开(公告)日：2021-12-23

申请号：US17283942

申请日：2019-12-20

Applicant: KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY

Inventor： Eun-Mi Kim ,  Ki-Suk Kim ,  Hyang-Ae Lee

IPC: C12N5/071 ,  C12M3/00 ,  C12N5/0786

Abstract: The present invention relates to a method for fabrication of a three-dimensional lung organoid comprising human stem cell-derived alveolar macrophages. Specifically, a lung organoid is fabricated by co-culturing cells not expressing the definitive endoderm marker CRCX4 according to a fabrication method of the present disclosure. The lung organoid comprises type 1 and type 2 alveolar epithelial cells as well as alveolar macrophages and realizes infectious or inflammatory responses unlike conventional lung organoids that contain no immune cells and as such, can be advantageously used in studying mechanisms of related lung diseases, excavating biomarkers, developing therapeutic agents, and so on.

公开(公告)号：US12173325B2

公开(公告)日：2024-12-24

申请号：US17283942

申请日：2019-12-20

Applicant: KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY

Inventor： Eun-Mi Kim ,  Ki-Suk Kim ,  Hyang-Ae Lee

IPC: C12N5/071 ,  C12M3/00 ,  C12N5/0786

Abstract: The present invention relates to a method for fabrication of a three-dimensional lung organoid comprising human stem cell-derived alveolar macrophages. Specifically, a lung organoid is fabricated by co-culturing cells not expressing the definitive endoderm marker CRCX4 according to a fabrication method of the present disclosure. The lung organoid comprises type 1 and type 2 alveolar epithelial cells as well as alveolar macrophages and realizes infectious or inflammatory responses unlike conventional lung organoids that contain no immune cells and as such, can be advantageously used in studying mechanisms of related lung diseases, excavating biomarkers, developing therapeutic agents, and so on.