公开(公告)号：US20150301052A1

公开(公告)日：2015-10-22

申请号：US14399853

申请日：2013-05-07

Applicant: KOWA COMPANY, LTD.

Inventor： Taisuke Hirono ,  Yuka Sugiura

IPC: G01N33/579

CPC classification number: G01N33/579 ,  G01N2333/96433 ,  G01N2333/96463 ,  G01N2400/24 ,  G01N2400/50

Abstract: Provided is a technique for achieving a higher measurement accuracy in the detection of an organism-originated physiologically active substance or the measurement of the concentration of the organism-originated physiologically active substance in a hyaluronic acid preparation. The present invention relates to a measurement method for detecting an organism-originated physiologically active substance or measuring the concentration of the organism-originated physiologically active substance in a hyaluronic acid preparation by mixing the hyaluronic acid preparation with an AL reagent to allow the organism-originated physiologically active substance in the hyaluronic acid preparation and an AL to react with each other. A mixed solution of the hyaluronic acid preparation with the AL reagent is diluted with an aqueous electrolyte solution, and a specific protein having a higher affinity for the organism-originated physiologically active substance than that of hyaluronic acid is added to the mixed solution.

Abstract translation: 提供了在透明质酸制剂中检测生物来源的生理活性物质或测定生物体起始生理活性物质的浓度的技术。 本发明涉及通过将透明质酸制剂与AL试剂混合以使生物来源的透明质酸制剂通过透明质酸制剂检测生物体产生的生理活性物质或测量生物体起始生理活性物质的浓度的测量方法 透明质酸制剂中的生理活性物质和AL彼此反应。 将透明质酸制剂与AL试剂的混合溶液用电解质水溶液稀释，向混合溶液中加入对生物体起始生理活性物质的亲和力高于透明质酸的特异性蛋白质。

公开(公告)号：US20150138552A1

公开(公告)日：2015-05-21

申请号：US14402948

申请日：2012-11-15

Applicant: KOWA COMPANY, LTD.

Inventor： Taisuke Hirono

IPC: G01N21/49 ,  G01N33/487

CPC classification number: G01N21/49 ,  G01N21/0332 ,  G01N21/51 ,  G01N21/82 ,  G01N33/487 ,  G01N2021/0357 ,  G01N2021/0367 ,  G01N2021/4707 ,  G01N2201/064

Abstract: With regard to the detection of a physiological active substance of biological origin and the measurement of its concentration in a sample, the invention provides a technique for moving gel particles that are produced in the sample without using a mechanical stirring member, and allows a highly accurate detection of the physiological active substance of biological origin and measurement of its concentration with a simple arrangement. By partial heating/cooling of a sample cell, thermal convection is generated within a mixture liquid in the sample cell, and as a result the gel particles that are produced in the mixture liquid are moved. In addition, based on the intensity of forward scattered light, the rate of change in the number of gel particles is measured.

Abstract translation: 关于生物来源的生物活性物质的检测及其在样品中的浓度的测定，本发明提供一种移动在样品中生产的凝胶颗粒而不使用机械搅拌构件的技术，并且允许高精度 检测生物来源的生理活性物质，并用简单的排列测定其浓度。 通过对样品池进行部分加热/冷却，在样品池内的混合液体中产生热对流，结果，在混合液中产生的凝胶粒子移动。 此外，基于前向散射光的强度，测定凝胶粒子数量的变化率。