公开(公告)号：US20240279696A1

公开(公告)日：2024-08-22

申请号：US17785813

申请日：2020-12-16

申请人： KYOWA HAKKO BIO CO., LTD.

发明人： Kenichiro TABATA ,  Shinsuke UCHIYA

IPC分类号： C12P19/00 ,  C12N9/12

CPC分类号： C12P19/00 ,  C12N9/1205 ,  C12Y207/01

摘要： An object of the invention is to provide a method for producing a lactose-containing oligosaccharide through more efficient fermentative production. According to the invention, using a microorganism having an ability of producing a protein which is modified by replacing a specific amino acid residue with a different amino acid residue and which has lactose permease activity, a lactose-containing oligosaccharide such as 2′-fucosyllactose can be produced more efficiently as compared to the case using a microorganism having an ability of producing the wild-type protein having lactose permease activity.

公开(公告)号：US20240318213A1

公开(公告)日：2024-09-26

申请号：US18547082

申请日：2022-02-18

申请人： KYOWA HAKKO BIO CO., LTD.

发明人： Kenichiro TABATA ,  Nozomu KAMADA

IPC分类号： C12P19/00 ,  C12N9/10 ,  C12P19/18

CPC分类号： C12P19/00 ,  C12N9/1051 ,  C12P19/18

摘要： An object of the present invention is to provide a modified protein having an α1,2-fucosyltransferase activity and a method for producing a fucose-containing carbohydrate using the protein or a microorganism having an ability to produce the protein. According to the present invention, a fucose-containing carbohydrate such as 2′-fucosyllactose can be more efficiently produced by using a protein having an α1,2-fucosyltransferase activity and being modified to substitute a specific amino acid residue with another amino acid residue, or a microorganism having the protein or an ability to produce the protein, compared to the case of using a wild-type protein having an α1,2-fucosyltransferase activity, or a microorganism having the protein or an ability to produce the protein.

公开(公告)号：US20240141402A1

公开(公告)日：2024-05-02

申请号：US18002349

申请日：2021-06-24

申请人： KYOWA HAKKO BIO CO., LTD.

发明人： Kenichiro TABATA ,  Naoto TSUDA ,  Yuugo ADACHI

IPC分类号： C12P21/02 ,  C07K14/00 ,  C12N9/00

CPC分类号： C12P21/02 ,  C07K14/001 ,  C12N9/93 ,  C12Y603/02

摘要： An object of the present invention is to provide a protein having dipeptide synthesizing activity with improved substrate specificity, and a method in which the protein or a microorganism having ability to produce the protein is used to efficiently produce a target dipeptide while reducing a by-product dipeptide produced in addition to the target dipeptide. According to the present invention, a protein consisting of an amino acid sequence obtained by substituting, with other amino acid residues, amino acid residues corresponding to one or more amino acid residues selected from the group consisting of amino acid residues at positions 107, 108, and 110 in an amino acid sequence set forth in SEQ ID NO: 2, or a mutant protein or a homologous protein of a protein consisting of the amino acid sequence set forth in SEQ ID NO: 2 is provided, and a microorganism producing the protein can be used to efficiently produce a dipeptide.