公开(公告)号：US20190169240A1

公开(公告)日：2019-06-06

申请号：US16216617

申请日：2018-12-11

申请人： KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION ,  HIROSHIMA UNIVERSITY

发明人： Takashi Yamamoto ,  Tetsushi Sakuma ,  Takahiro Nakamura ,  Yusuke Yagi ,  Yasuyuki Okawa

IPC分类号： C07K14/415 ,  C12N15/82 ,  C12N15/85 ,  C12N9/22

摘要： The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an α-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an α-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (−2) A.A. contained in L of the formula 1, the aforementioned object could be achieved. (Helix A)-X-(Helix B)-L  (Formula 1)

公开(公告)号：US20210324019A1

公开(公告)日：2021-10-21

申请号：US17365090

申请日：2021-07-01

申请人： KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION ,  HIROSHIMA UNIVERSITY

发明人： Takashi Yamamoto ,  Tetsushi Sakuma ,  Takahiro Nakamura ,  Yusuke Yagi ,  Yasuyuki Okawa

IPC分类号： C07K14/415 ,  C12N9/22 ,  C12N15/82 ,  C12N15/85

摘要： The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an α-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an α-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (-2) A.A. contained in L of the formula 1, the aforementioned object could be achieved. (Helix A)-X-(Helix B)-L  (Formula 1)

公开(公告)号：US10189879B2

公开(公告)日：2019-01-29

申请号：US14785952

申请日：2014-04-22

申请人： KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION ,  HIROSHIMA UNIVERSITY

发明人： Takashi Yamamoto ,  Tetsushi Sakuma ,  Takahiro Nakamura ,  Yusuke Yagi ,  Yasuyuki Okawa

IPC分类号： C12N9/22 ,  C07K14/415 ,  C12N15/82 ,  C12N15/85

摘要： The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an α-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an α-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (−2) A. A. contained in L of the formula 1, the aforementioned object could be achieved. (Helix A)-X-(Helix B)-L  (Formula 1)

公开(公告)号：US20160075744A1

公开(公告)日：2016-03-17

申请号：US14785952

申请日：2014-04-22

申请人： KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION ,  HIROSHIMA UNIVERSITY

发明人： Takashi Yamamoto ,  Tetsushi Sakuma ,  Takahiro Nakamura ,  Yusuke Yagi ,  Yasuyuki Okawa

IPC分类号： C07K14/415 ,  C12N15/85 ,  C12N9/22

CPC分类号： C07K14/415 ,  C07K2319/80 ,  C12N9/22 ,  C12N15/8213 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/85 ,  C12Y301/21004

摘要： The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an α-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an α-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (-2) A.A. contained in L of the formula 1, the aforementioned object could be achieved. (Helix A)-X-(Helix B)-L  (Formula 1)

摘要翻译： 本发明的目的是通过分析PPR蛋白与DNA的结合，预测PPR基序的RNA识别规则也可以用于DNA的识别，找到显示出这种特征的PPR蛋白质。 根据本发明，揭示了含有1个以上，优选2〜30个，更优选5〜25个的DNA碱基选择性或DNA碱基序列特异性结合的蛋白质， 最优选9至15个具有下式1结构的PPR基序（其中，在式1中，螺旋A是可以形成α-螺旋结构的部分; X不存在，或者是由 1至9个氨基酸;螺旋B是可以形成α-螺旋结构的部分; L是由2至7个氨基酸组成的部分），并且具有对应于DNA碱基或DNA碱基的氨基酸的特异性组合 序列作为1号AA，4号AA的三个位置的氨基酸，在式1和No.“ii”（-2）AA的螺旋A中 包含在式1的L中，可以实现上述目的。 （螺旋A）-X-（螺旋B）-L（式1）

公开(公告)号：US20220064229A1

公开(公告)日：2022-03-03

申请号：US17365292

申请日：2021-07-01

申请人： KYUSHU UNIVERSITY, NAT'L UNIVERSITY CORPORATION ,  HIROSHIMA UNIVERSITY

发明人： Takashi Yamamoto ,  Tetsushi Sakuma ,  Takahiro Nakamura ,  Yusuke Yagi ,  Yasuyuki Okawa

IPC分类号： C07K14/415 ,  C12N9/22 ,  C12N15/82 ,  C12N15/85

摘要： A method for designing a protein capable of binding in a DNA base selective manner or DNA base sequence specific manner is provided. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an α-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an α-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (-2) A.A. contained in L of the formula 1, the aforementioned object could be achieved. (Helix A)-X-(Helix B)-L  (Formula 1)