公开(公告)号：US20230374529A1

公开(公告)日：2023-11-23

申请号：US18179798

申请日：2023-03-07

申请人： CORTEVA AGRISCIENCE LLC

发明人： DAVID R. CORBIN ,  WEI CHEN ,  STEPHEN NOVAK ,  RYAN M. LEE ,  SANDEEP KUMAR ,  ANDREW ASBERRY ,  ANDREW F. WORDEN

IPC分类号： C12N15/82 ,  C12N9/22 ,  C12N15/90

CPC分类号： C12N15/8213 ,  C12N9/22 ,  C12Y301/21004 ,  C12N15/8261 ,  C12N15/8209 ,  C12N15/8286 ,  C12N15/8274 ,  C12N15/90 ,  Y02A40/146

摘要： Disclosed herein are methods and compositions for the repair of site specific nuclease binding sites by targeted integration and/or targeted excision of one or more sequences into a cell.

公开(公告)号：US11725218B2

公开(公告)日：2023-08-15

申请号：US17493655

申请日：2021-10-04

申请人： Sangamo Therapeutics, Inc.

发明人： Yannick Doyon ,  Jeffrey C. Miller

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N9/10 ,  C12N15/52 ,  C12N15/85 ,  C07K14/47 ,  C07K7/06

CPC分类号： C12N15/907 ,  C07K7/06 ,  C07K14/47 ,  C12N9/10 ,  C12N9/22 ,  C12N15/52 ,  C12N15/85 ,  C07K2319/50 ,  C07K2319/80 ,  C07K2319/81 ,  C12Y301/21004

摘要： Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.

公开(公告)号：US11674128B2

公开(公告)日：2023-06-13

申请号：US15619518

申请日：2017-06-11

申请人： Tsinghua University

发明人： Zhen Xie ,  Dacheng Ma

IPC分类号： C12N9/22 ,  C12N15/86 ,  C12N15/11

CPC分类号： C12N9/22 ,  C12N15/86 ,  C12Y301/21004 ,  C07K2319/70 ,  C12N15/111 ,  C12N2310/20 ,  C12N2310/344 ,  C12N2310/3519 ,  C12N2750/14143

摘要： The presently claimed invention offers programmable and precise regulation of Cas9 functions by utilizing a set of compact Cas9 derivatives created by deleting conserved HNH and/or REC-C domains based on the structural information across variant class 2 CRISPR effectors. In addition, a novel strategy for engineering the dimeric gRNA-guided nuclease by splitting the mini-dSaCas9 and fusing the FokI domain right after the split point is claimed to increase the on-target DNA cleavage efficiency and potentially reduce the off-target effect because of a closer proximity of dimeric Fold nuclease to the target sequence. By combining the optimized and compact gRNA expression cassette and the downsized SaCas9 derivatives, the entire CRISPR/Cas system with different effector domains for transactivation, DNA cleavage and base editing is loaded into a single AAV virus. Such an all-in-one AAV-CRISPR/Cas9 system will be particularly appealing in biomedical applications that require safe and efficient delivery in vivo.

公开(公告)号：US20180208957A1

公开(公告)日：2018-07-26

申请号：US15570130

申请日：2016-04-29

申请人： CureVac AG

发明人： Tilmann ROOS ,  Benyamin YAZDAN PANAH ,  Martin KUNZE

IPC分类号： C12P19/34 ,  C12N11/10 ,  C12N11/06 ,  C12M1/40

CPC分类号： C12P19/34 ,  C12M21/18 ,  C12N9/22 ,  C12N11/06 ,  C12N11/10 ,  C12Y301/21004

摘要： The present invention relates to a method for in vitro transcription of a linear template DNA which is produced using an immobilized restriction endonuclease. The invention also relates to mutated restriction enzymes which are suitable for immobilization and a solid support to which these restriction enzymes are immobilized. Further, the present invention relates to an enzyme reactor containing said immobilized restriction endonuclease which enzyme reactor can be used for preparing linearized template DNA. Finally, the present invention relates to the use of said enzyme reactor for preparing a linear template DNA for in vitro transcription. In addition, the present invention relates to a kit comprising the immobilized restriction endonuclease.

公开(公告)号：US20180163188A1

公开(公告)日：2018-06-14

申请号：US15619518

申请日：2017-06-11

申请人： Tsinghua University

发明人： Zhen Xie ,  Dacheng Ma

IPC分类号： C12N9/22 ,  C12N15/11 ,  C12N15/86 ,  C07K14/00

CPC分类号： C12N9/22 ,  C07K2319/70 ,  C12N15/111 ,  C12N15/86 ,  C12N2310/20 ,  C12N2310/344 ,  C12N2310/3519 ,  C12N2750/14143 ,  C12Y301/21004

摘要： The presently claimed invention offers programmable and precise regulation of Cas9 functions by utilizing a set of compact Cas9 derivatives created by deleting conserved HNH and/or REC-C domains based on the structural information across variant class 2 CRISPR effectors. In addition, a novel strategy for engineering the dimeric gRNA-guided nuclease by splitting the mini-dSaCas9 and fusing the FokI domain right after the split point is claimed to increase the on-target DNA cleavage efficiency and potentially reduce the off-target effect because of a closer proximity of dimeric FokI nuclease to the target sequence. By combining the optimized and compact gRNA expression cassette and the downsized SaCas9 derivatives, the entire CRISPR/Cas system with different effector domains for transactivation, DNA cleavage and base editing is loaded into a single AAV virus. Such an all-in-one AAV-CRISPR/Cas9 system will be particularly appealing in biomedical applications that require safe and efficient delivery in vivo.

公开(公告)号：US09914940B2

公开(公告)日：2018-03-13

申请号：US15014372

申请日：2016-02-03

申请人： Sangamo BioSciences, Inc.

发明人： Russell DeKelver ,  Philip D. Gregory ,  David Paschon ,  Phillip Tam ,  Fyodor Urnov

IPC分类号： C12N15/11 ,  C12N15/90 ,  C12N15/10 ,  C07K14/435 ,  C12N9/22 ,  C12N15/113

CPC分类号： C12N15/907 ,  C07K14/435 ,  C07K14/43595 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/1082 ,  C12N15/113 ,  C12N2800/10 ,  C12N2800/30 ,  C12Y301/21004

摘要： Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest.

公开(公告)号：US09902968B2

公开(公告)日：2018-02-27

申请号：US14203090

申请日：2014-03-10

申请人： KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHO ,  THE UNIVERSITY OF TOKYO

发明人： Nobuhiko Muramoto ,  Hiroki Sugimoto ,  Norihiro Mitsukawa ,  Kunihiro Ohta ,  Kazuto Kugou

IPC分类号： C12N15/82 ,  C12N9/22

CPC分类号： C12N15/8261 ,  C12N9/22 ,  C12Y301/21004 ,  Y02A40/146

摘要： A method of increasing the biomass of a plant that includes planting a plant having plant cells carrying an exogenous gene that encodes a thermophilic restriction enzyme that promotes double-stranded DNA breakage, and growing the plant at least until after true leaf development, wherein the mean biomass of plants having the plant cells carrying the exogenous gene that are grown at least until after true leaf development is increased in comparison with the mean biomass of plants of the same species that do not carry the exogenous gene that are grown for the same amount of time.

公开(公告)号：US20170327805A1

公开(公告)日：2017-11-16

申请号：US15415431

申请日：2017-01-25

申请人： The General Hospital Corporation

发明人： J. Keith Joung ,  Shengdar Tsai

IPC分类号： C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N2310/20

CPC分类号： C12N15/102 ,  C07K14/005 ,  C07K14/195 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/80 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/1031 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85 ,  C12N2310/20 ,  C12N2710/00033 ,  C12N2770/00033 ,  C12N2800/80 ,  C12Y114/11 ,  C12Y201/01 ,  C12Y301/00 ,  C12Y301/21004

摘要： Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.

公开(公告)号：US09567604B2

公开(公告)日：2017-02-14

申请号：US14213723

申请日：2014-03-14

申请人： The General Hospital Corporation

发明人： J. Keith Joung ,  Jeffry D. Sander ,  Morgan Maeder ,  Yanfang Fu

IPC分类号： C07H21/02 ,  C12N15/00 ,  A61K48/00 ,  C12N15/85 ,  C12N9/22 ,  C12N15/63 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01

CPC分类号： C12N15/102 ,  C07K14/005 ,  C07K14/195 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/80 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/1031 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85 ,  C12N2310/20 ,  C12N2710/00033 ,  C12N2770/00033 ,  C12N2800/80 ,  C12Y114/11 ,  C12Y201/01 ,  C12Y301/00 ,  C12Y301/21004

摘要： Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).

摘要翻译： 使用截短的引导RNA（tru-gRNA）增加RNA指导基因组编辑的特异性的方法，例如使用CRISPR / Cas9系统的编辑。

公开(公告)号：US20160273002A1

公开(公告)日：2016-09-22

申请号：US15031996

申请日：2014-10-24

申请人： CELLECTIS

发明人： Philippe DUCHATEAU ,  Alexandre JUILLERAT

IPC分类号： C12N15/90 ,  C07K14/195 ,  C12N9/22

CPC分类号： C12N15/907 ,  A61K38/00 ,  A61K48/00 ,  A61K48/005 ,  C07K14/195 ,  C07K2319/80 ,  C12N9/22 ,  C12Y301/00 ,  C12Y301/21004

摘要： The present invention is in the field of genetic editing tools and methods of genetic engineering. It relates to the engineering of rare-cutting endonucleases designed to contract highly repetitive motives in chromosomes, which are at the origin of certain genetic diseases, in particular the so-called “triplet repeat diseases”, such as the Huntington disease. The invention encompasses the method for contracting the repetitive motives, the rare-cutting endonucleases for use to contract repetitive motives in a gene subjected to repeat disorder, the polynucleotides and vectors encoding thereof as well as the resulting pharmaceutical compositions.

摘要翻译： 本发明在遗传编辑工具和基因工程方法领域。 它涉及设计用于在染色体中收缩高度重复动机的稀有内切核酸酶的工程，这些动机是某些遗传疾病的起源，特别是所谓的“三重重复疾病”，如亨廷顿疾病。 本发明包括用于收缩重复动机的方法，用于在重复病症的基因中重复动作的稀有内切核酸酶，编码它们的多核苷酸和载体以及所得药物组合物。