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公开(公告)号：US20100248348A1
公开(公告)日：2010-09-30
申请号：US12692059
申请日：2010-01-22
IPC分类号： C12M1/34
CPC分类号： C12Q1/6851 , B01L7/52 , C12Q2537/165
摘要： The present invention relates to calculation of an amount equivalent to an initial template amount in a test sample by fitting a theoretical expression to an amount equivalent to an amplification amount of target nucleic acid for each thermal cycle number, wherein the theoretical expression includes an environmental coefficient as an exponent parameter and a parameter of the amount equivalent to the initial template amount, and includes at least one parameter among an amount equivalent to a saturation amount upon the target nucleic acid amplification, a reaction acceleration coefficient, and a reaction inhibition coefficient.
摘要翻译： 本发明涉及通过将理论表达式与每个热循环次数的靶核酸的扩增量相当的量来计算与试验样品中的初始模板量相当的量，其中理论表达式包括环境系数 作为指数参数和等于初始模板量的量的参数，并且包括与靶核酸扩增相当的饱和量的量，反应加速系数和反应抑制系数中的至少一个参数。
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公开(公告)号：US20110008878A1
公开(公告)日：2011-01-13
申请号：US12692240
申请日：2010-01-22
IPC分类号： C12M1/34
CPC分类号： C12Q1/686 , C12Q2545/101
摘要： The present invention relates to calculation of an initial template amount in a test sample by fitting a theoretical expression to detection intensity of target nucleic acid corresponding to thermal cycle number, wherein the theoretical expression includes an environmental coefficient as an exponent parameter, a parameter of the initial template amount in the test sample, and terms for internal standard correction and baseline correction of the detection intensity of the amplification amount of the target nucleic acid, and includes at least one parameter among a saturation amount upon the target nucleic acid amplification, a reaction acceleration coefficient, and a reaction inhibition coefficient.
摘要翻译： 本发明涉及通过将理论表达式拟合到对应于热循环次数的靶核酸的检测强度来计算测试样品中的初始模板量，其中理论表达式包括作为指数参数的环境系数， 测试样品中的初始模板量，目标核酸扩增量的检测强度的内标校正和基线校正的术语，并且包括目标核酸扩增后的饱和量中的至少一个参数，反应 加速系数和反应抑制系数。
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