公开(公告)号：US06372476B1

公开(公告)日：2002-04-16

申请号：US09383318

申请日：1999-08-26

申请人： Karima Srih Belguith ,  Radhouane Ellouz ,  Samir Bejar

发明人： Karima Srih Belguith ,  Radhouane Ellouz ,  Samir Bejar

IPC分类号： C12N990

CPC分类号： C12N9/92 ,  C12Y503/01005

摘要： Disclosed are isolated polypeptides having glucose isomerase activity selected from: (a) a polypeptide having an amino acid sequence which has at least 95% identity with amino acids of SEQ ID NO:2; (b) a variant of the polypeptide having an amino acid sequence of SEQ ID NO:2 comprising a substitution, deletion, and/or insertion of one or more amino acids; (c) a fragment of (a) that has glucose isomerase activity; and (d) a polypeptide having a pH optimum in the range of 5.7 to 6.3 at 60° C., a pH optimum in the range of 6.1 to 6.7 at 90° C and a temperature optimum of above 90° C. Also disclosed are isolated nucleic acid sequences encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

摘要翻译： 公开了具有选自以下的葡萄糖异构酶活性的分离的多肽：（a）具有与SEQ ID NO：2的氨基酸具有至少95％同一性的氨基酸序列的多肽;（b）具有氨基酸的多肽的变体 包含一个或多个氨基酸的取代，缺失和/或插入的SEQ ID NO：2的序列;（c）具有葡萄糖异构酶活性的（a）的片段; 和（d）在60℃下pH最佳为5.7至6.3的多肽，在90℃下最佳为6.1至6.7，最适温度为90℃以上的pH最佳。 分离的编码多肽的核酸序列，核酸构建体，载体和包含核酸序列的宿主细胞以及产生和使用多肽的方法。