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1.发明授权Polypeptides having glucose isomerase activity and nucleic acids encoding same 失效具有葡萄糖异构酶活性的多肽和编码其的核酸公开(公告)号：US06372476B1
公开(公告)日：2002-04-16
申请号：US09383318
申请日：1999-08-26
IPC分类号： C12N990
CPC分类号： C12N9/92 , C12Y503/01005
摘要： Disclosed are isolated polypeptides having glucose isomerase activity selected from: (a) a polypeptide having an amino acid sequence which has at least 95% identity with amino acids of SEQ ID NO:2; (b) a variant of the polypeptide having an amino acid sequence of SEQ ID NO:2 comprising a substitution, deletion, and/or insertion of one or more amino acids; (c) a fragment of (a) that has glucose isomerase activity; and (d) a polypeptide having a pH optimum in the range of 5.7 to 6.3 at 60° C., a pH optimum in the range of 6.1 to 6.7 at 90° C and a temperature optimum of above 90° C. Also disclosed are isolated nucleic acid sequences encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
摘要翻译： 公开了具有选自以下的葡萄糖异构酶活性的分离的多肽：（a）具有与SEQ ID NO：2的氨基酸具有至少95％同一性的氨基酸序列的多肽;（b）具有氨基酸的多肽的变体 包含一个或多个氨基酸的取代，缺失和/或插入的SEQ ID NO：2的序列;（c）具有葡萄糖异构酶活性的（a）的片段; 和（d）在60℃下pH最佳为5.7至6.3的多肽，在90℃下最佳为6.1至6.7，最适温度为90℃以上的pH最佳。 分离的编码多肽的核酸序列，核酸构建体，载体和包含核酸序列的宿主细胞以及产生和使用多肽的方法。
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2.发明申请Polypeptides Having L-Arabinose Isomerase Activity Exhibiting Minimum Dependence on Metal Ions for Its Activity and for Thermostability and Nucleic Acids Encoding the Same 审中-公开具有L-Arabinose异构酶活性的多肽表现出对金属离子的活性和热稳定性的最小依赖性及其编码的核酸公开(公告)号：US20100173366A1
公开(公告)日：2010-07-08
申请号：US11794510
申请日：2005-06-28
申请人： Moez Rhimi , Hichem Chouayekh , Mamdouh Ben Ali , Belgacem Naili , Sonia Jemli , Samir Bejar
发明人： Moez Rhimi , Hichem Chouayekh , Mamdouh Ben Ali , Belgacem Naili , Sonia Jemli , Samir Bejar
摘要： The invention concerns identification of a gene encoding a novel L-arabinose isomerase of the Bacillus stearothernivphilus strain US 100 (L-AI US 100), a L-arabinose isomerase expressed from said gene, recombinant vectors harbouring said gene, microorganisms transformed with said vector, a protocol for preparing and purifying said recombinant protein, biochemical and kinetic characterization of said recombinant enzyme and a method for bioconversion of a D-galactose solution into a solution rich in D-tagatose using said polypeptide. This novel protein has original characteristics, in particular its independence from metal ions for its activity and its low need for such ions for its thermostability, as well as its potential for isomerizing D-galactose into D-tagatose with great efficacy of about 48% after 7 hours at 70° C.
摘要翻译： 本发明涉及鉴定编码嗜热链球菌芽孢杆菌菌株US100（L-AI US100）的新型L-阿拉伯糖异构酶的基因，从所述基因表达的L-阿拉伯糖异构酶，含有所述基因的重组载体，用所述载体转化的微生物 ，用于制备和纯化所述重组蛋白质的方案，所述重组酶的生物化学和动力学表征以及使用所述多肽将D-半乳糖溶液生物转化成富含D-塔格糖的溶液的方法。 这种新型蛋白质具有原始特征，特别是其与金属离子的活性无关，并且其对这种离子的热稳定性的低需求，以及其将D-半乳糖异构化为D-塔格糖的潜力，其效力约为48％ 70°C 7小时
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