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1.发明授权Method for enriching and/or separating prokaryotic DNA using a protein that specifically bonds to unmethylated DNA containing CpG-motifs 失效使用特异性结合含有CpG基序的未甲基化DNA的蛋白质来富集和/或分离原核DNA的方法公开(公告)号：US08481262B2
公开(公告)日：2013-07-09
申请号：US10591633
申请日：2005-03-02
申请人： Karl-Hermann Schmidt , Eberhard Straube , Stefan Russwurm , Hans-Peter Deigner , Svea Sachse , Marc Lehmann
发明人： Karl-Hermann Schmidt , Eberhard Straube , Stefan Russwurm , Hans-Peter Deigner , Svea Sachse , Marc Lehmann
CPC分类号： C12N15/1006 , C12N15/1003
摘要： The invention relates to a method for separating and/or enriching prokaryotic DNA, comprising the following steps: a) contacting of at least one prokaryotic DNA that is in solution with a protein that bonds specifically to prokaryotic DNA, the protein being 25%-35% homologous with the wild-type CGPB protein, thus forming a protein-DNA complex; and b) separation of the complex. The invention also relates to a kit for carrying out said method.
摘要翻译： 本发明涉及分离和/或富集原核DNA的方法，包括以下步骤：a）将至少一种在溶液中的原核DNA与特异性结合于原核DNA的蛋白质接触，所述蛋白质为25％-35 与野生型CGPB蛋白质同源，从而形成蛋白质-DNA复合物; 和b）复合物的分离。 本发明还涉及一种用于执行所述方法的套件。
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公开(公告)号：US08062854B2
公开(公告)日：2011-11-22
申请号：US10528235
申请日：2003-08-08
CPC分类号： C12N15/1006
摘要： A method is described for enriching procaryotic DNA, said method including the steps of contacting at least one procaryotic DNA with at least one protein or polypeptide which is capable of specifically binding to non-methylated CpG motifs, and separating the protein/polypeptide-DNA complex. Moreover, the application relates to a kit for carrying out said method.
摘要翻译： 描述了用于富集原核DNA的方法，所述方法包括使至少一种原核DNA与至少一种能够特异性结合非甲基化CpG基序的蛋白质或多肽接触并分离蛋白质/多肽-DNA复合物的步骤 。 此外，本申请涉及用于执行所述方法的套件。
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公开(公告)号：US08288115B2
公开(公告)日：2012-10-16
申请号：US13253661
申请日：2011-10-05
CPC分类号： C12N15/1006
摘要： A method is described for enriching procaryotic DNA, said method including the steps of contacting at least one procaryotic DNA with at least one protein or polypeptide which is capable of specifically binding to non-methylated CpG motifs, and separating the protein/polypeptide-DNA complex. Moreover, the application relates to a kit for carrying out said method.
摘要翻译： 描述了用于富集原核DNA的方法，所述方法包括使至少一种原核DNA与至少一种能够特异性结合非甲基化CpG基序的蛋白质或多肽接触并分离蛋白质/多肽-DNA复合物的步骤 。 此外，本申请涉及用于执行所述方法的套件。
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4.发明申请Method for Enriching and/or Separating Prokaryotic Dna Using a Protein that Specifically Bonds to Unmethylated Dna Containing Cpg-Motives 失效使用特异性结合含有Cpg动机的非甲基化Dna的蛋白质来丰富和/或分离原核Dna的方法公开(公告)号：US20080003568A1
公开(公告)日：2008-01-03
申请号：US10591633
申请日：2005-03-02
申请人： Karl-Hermann Schmidt , Eberhard Straube , Stefan Russwurm , Hans-Peter Deigner , Svea Sachse , Marc Lehmann
发明人： Karl-Hermann Schmidt , Eberhard Straube , Stefan Russwurm , Hans-Peter Deigner , Svea Sachse , Marc Lehmann
CPC分类号： C12N15/1006 , C12N15/1003
摘要： The invention relates to a method for separating and/or enriching prokaryotic DNA, comprising the following steps: a) contacting of at least one prokaryotic DNA that is in solution with a protein that bonds specifically to prokaryotic DNA, said protein being 25%-35% homologous with the wild-type CGPB protein, thus forming a protein-DNA complex; and b) separation of the complex. The invention also relates to a kit for carrying out said method.
摘要翻译： 本发明涉及分离和/或富集原核DNA的方法，包括以下步骤：a）将至少一种在溶液中的原核DNA与特异性结合于原核DNA的蛋白质接触，所述蛋白质为25％-35 与野生型CGPB蛋白质同源，从而形成蛋白质-DNA复合物; 和b）复合物的分离。 本发明还涉及一种用于执行所述方法的套件。
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公开(公告)号：US20100316993A1
公开(公告)日：2010-12-16
申请号：US10528235
申请日：2003-08-08
CPC分类号： C12N15/1006
摘要： A method is described for enriching procaryotic DNA, said method including the steps of contacting at least one procaryotic DNA with at least one protein or polypeptide which is capable of specifically binding to non-methylated CpG motifs, and separating the protein/polypeptide-DNA complex. Moreover, the application relates to a kit for carrying out said method.
摘要翻译： 描述了用于富集原核DNA的方法，所述方法包括使至少一种原核DNA与至少一种能够特异性结合非甲基化CpG基序的蛋白质或多肽接触并分离蛋白质/多肽-DNA复合物的步骤 。 此外，本申请涉及用于执行所述方法的套件。
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