公开(公告)号：US5834184A

公开(公告)日：1998-11-10

申请号：US442461

申请日：1995-05-17

申请人： Kazuo Harada ,  Shelley S. Martin ,  Alan Frankel

发明人： Kazuo Harada ,  Shelley S. Martin ,  Alan Frankel

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N15/10 ,  C12Q1/68 ,  C12N1/20

CPC分类号： C12Q1/6897 ,  C12N15/1048 ,  C07K2319/00

摘要： The invention provides methods and kits suitable for large-scale in vivo screening of polypeptides for specific binding affinity to a selected RNA recognition sequence. Polypeptide(s) are screened as fusion proteins with a polypeptide having antitermination activity. Polypeptides having binding activity are identified by their capacity to activate transcription of a reporter gene via binding of the polypeptide being screened to the selected RNA recognition sequence. The invention also provides methods of screening RNA molecules for binding to a selected polypeptide.

摘要翻译： 本发明提供了适用于大规模体内筛选多肽的方法和试剂盒，用于对选定的RNA识别序列的特异性结合亲和力。 多肽作为具有抗发芽活性的多肽的融合蛋白进行筛选。 具有结合活性的多肽通过其被筛选的多肽与选定的RNA识别序列的结合来激活报道基因的转录的能力来鉴定。 本发明还提供筛选RNA分子以结合所选多肽的方法。