公开(公告)号：US20050260710A1

公开(公告)日：2005-11-24

申请号：US11092988

申请日：2005-03-30

申请人： Kazuo Suzuki ,  Masahiro Furutani ,  Kenji Yamamoto ,  Naohito Ohno ,  Kei Takahashi ,  Yasuaki Aratani ,  Akiko Togi

发明人： Kazuo Suzuki ,  Masahiro Furutani ,  Kenji Yamamoto ,  Naohito Ohno ,  Kei Takahashi ,  Yasuaki Aratani ,  Akiko Togi

IPC分类号： C07K16/00 ,  C07K16/44 ,  C12N1/21 ,  C12N15/74 ,  C12P21/06

CPC分类号： C07K16/00 ,  A61K2039/505 ,  C07K2317/52 ,  C07K2317/622 ,  C07K2319/00

摘要： The purpose of the present invention is to provide methods for producing recombinant polyclonal immunoglobulins using recombinant DNA techniques, which provides constant supply of immunoglobulin preparations with minimum risk of infection. A method of the present invention comprises the following steps: (1) isolating a plurality of types of genes from cDNAs derived from tissues or cells expressing immunoglobulins, said genes encoding a plurality of types of polypeptides respectively, and said polypeptides containing a plurality of types of immunoglobulin variable regions respectively, and preparing mixture of the genes; (2) preparing a plurality of types of recombinant vectors into which a plurality of types of genes are introduced respectively by contacting said mixture of the genes with vectors, and preparing mixture of the recombinant vectors; (3) preparing a plurality of types of transformants into which a plurality of types of recombinant vectors are introduced respectively by contacting said mixture of the recombinant vectors with host cells, and preparing mixture of the transformants; and (4) culturing said mixture of the transformants, and obtaining mixture of polypeptides from the transformants culture, wherein said polypeptides contain a plurality of types of immunoglobulin variable regions respectively.

摘要翻译： 本发明的目的是提供使用重组DNA技术制备重组多克隆免疫球蛋白的方法，其提供具有最小感染风险的免疫球蛋白制剂的恒定供应。 本发明的方法包括以下步骤：（1）从衍生自表达免疫球蛋白的组织或细胞的cDNA分离多种类型的基因，所述基因分别编码多种类型的多肽，所述多肽含有多种类型 的免疫球蛋白可变区，并制备基因的混合物; （2）通过将所述基因混合物与载体接触，制备多种类型的重组载体，其中分别导入多种类型的基因，并制备重组载体的混合物; （3）通过使重组载体的混合物与宿主细胞接触，制备分别引入多种类型的重组载体的多种类型的转化体，并制备转化体的混合物; （4）培养所述转化体的所述混合物，并从所述转化体培养物获得多肽的混合物，其中所述多肽分别含有多种类型的免疫球蛋白可变区。