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    Methods for producing recombinant polyclonal immunoglobulins
    1.
    发明申请
    Methods for producing recombinant polyclonal immunoglobulins 审中-公开
    重组多克隆免疫球蛋白的制备方法

    公开(公告)号：US20050260710A1

    公开(公告)日：2005-11-24

    申请号：US11092988

    申请日：2005-03-30

    申请人： Kazuo Suzuki ,  Masahiro Furutani ,  Kenji Yamamoto ,  Naohito Ohno ,  Kei Takahashi ,  Yasuaki Aratani ,  Akiko Togi

    发明人： Kazuo Suzuki ,  Masahiro Furutani ,  Kenji Yamamoto ,  Naohito Ohno ,  Kei Takahashi ,  Yasuaki Aratani ,  Akiko Togi

    IPC分类号： C07K16/00 ,  C07K16/44 ,  C12N1/21 ,  C12N15/74 ,  C12P21/06

    CPC分类号： C07K16/00 ,  A61K2039/505 ,  C07K2317/52 ,  C07K2317/622 ,  C07K2319/00

    摘要： The purpose of the present invention is to provide methods for producing recombinant polyclonal immunoglobulins using recombinant DNA techniques, which provides constant supply of immunoglobulin preparations with minimum risk of infection. A method of the present invention comprises the following steps: (1) isolating a plurality of types of genes from cDNAs derived from tissues or cells expressing immunoglobulins, said genes encoding a plurality of types of polypeptides respectively, and said polypeptides containing a plurality of types of immunoglobulin variable regions respectively, and preparing mixture of the genes; (2) preparing a plurality of types of recombinant vectors into which a plurality of types of genes are introduced respectively by contacting said mixture of the genes with vectors, and preparing mixture of the recombinant vectors; (3) preparing a plurality of types of transformants into which a plurality of types of recombinant vectors are introduced respectively by contacting said mixture of the recombinant vectors with host cells, and preparing mixture of the transformants; and (4) culturing said mixture of the transformants, and obtaining mixture of polypeptides from the transformants culture, wherein said polypeptides contain a plurality of types of immunoglobulin variable regions respectively.

    摘要翻译： 本发明的目的是提供使用重组DNA技术制备重组多克隆免疫球蛋白的方法，其提供具有最小感染风险的免疫球蛋白制剂的恒定供应。 本发明的方法包括以下步骤：（1）从衍生自表达免疫球蛋白的组织或细胞的cDNA分离多种类型的基因，所述基因分别编码多种类型的多肽，所述多肽含有多种类型 的免疫球蛋白可变区，并制备基因的混合物; （2）通过将所述基因混合物与载体接触，制备多种类型的重组载体，其中分别导入多种类型的基因，并制备重组载体的混合物; （3）通过使重组载体的混合物与宿主细胞接触，制备分别引入多种类型的重组载体的多种类型的转化体，并制备转化体的混合物; （4）培养所述转化体的所述混合物，并从所述转化体培养物获得多肽的混合物，其中所述多肽分别含有多种类型的免疫球蛋白可变区。

    Method of producing target protein, fusion protein and gene thereof, protein consisting of partial sequence of intein and gene thereof, expression vector, and transformant
    2.
    发明申请
    Method of producing target protein, fusion protein and gene thereof, protein consisting of partial sequence of intein and gene thereof, expression vector, and transformant 审中-公开
    产生靶蛋白，融合蛋白及其基因的方法，由内含肽部分序列及其基因组成的蛋白质，表达载体和转化体

    公开(公告)号：US20070092937A1

    公开(公告)日：2007-04-26

    申请号：US10554751

    申请日：2004-04-28

    申请人： Akiko Togi ,  Masahiro Furutani

    发明人： Akiko Togi ,  Masahiro Furutani

    IPC分类号： C07K14/195 ,  C12P21/06 ,  C07H21/04 ,  C12N15/74 ,  C12N1/21 ,  C12N1/18

    CPC分类号： C12P21/06 ,  C07K2319/23 ,  C07K2319/35 ,  C07K2319/43 ,  C07K2319/61 ,  C07K2319/92 ,  C12N15/62

    摘要： It is intended to provide techniques whereby a target protein is efficiently obtained as a normal protein even in the case of a protein which can be hardly expressed by usual recombinant DNA techniques. A fusion protein composed of a molecular chaperone, an intervening protein having an activity of cleaving a peptide bond, and a target protein is prepared, from which fusion protein the target protein is cleaved by the action of the intervening protein having the activity of cleaving a peptide bond. Examples of the intervening protein having the activity of cleaving a peptide bond include an intein and a protein consisting of a partial sequence of the intein.

    摘要翻译： 旨在提供即使在通常的重组DNA技术几乎不能表达的蛋白质的情况下也可有效地获得靶蛋白作为正常蛋白质的技术。 制备由分子伴侣组成的融合蛋白，具有切割肽键的活性的中间蛋白质和靶蛋白质，通过具有切割活性的中间蛋白质的作用将靶蛋白切割成融合蛋白 肽键。 具有切割肽键活性的中间蛋白质的实例包括内含肽和由内含肽的部分序列组成的蛋白质。

    Chaperonin-target protein complex, method of producing the same, method of stabilizing target protein, method of immobilizing target protein, method of analyzing the structure of target protein, sustained-release formulation, and method of producing antibody against target protein
    3.
    发明申请
    Chaperonin-target protein complex, method of producing the same, method of stabilizing target protein, method of immobilizing target protein, method of analyzing the structure of target protein, sustained-release formulation, and method of producing antibody against target protein 审中-公开
    伴侣蛋白靶蛋白复合物，其制备方法，靶蛋白稳定化方法，固定靶蛋白的方法，分析靶蛋白结构的方法，缓释制剂及产生针对靶蛋白的抗体的方法

    公开(公告)号：US20070059794A1

    公开(公告)日：2007-03-15

    申请号：US10554747

    申请日：2004-04-28

    申请人： Akira Ideno ,  Jun-ichi Hata ,  Akiko Togi ,  Masahiro Furutani

    发明人： Akira Ideno ,  Jun-ichi Hata ,  Akiko Togi ,  Masahiro Furutani

    IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N9/64

    CPC分类号： C12N15/62 ,  A61K38/1709 ,  C07K2319/20 ,  C07K2319/35 ,  C07K2319/40 ,  C07K2319/705 ,  G01N33/6803

    摘要： The present invention provides a chaperonin-target protein complex and a method of producing the same, and a method of stabilizing the target protein, a method of immobilizing the target protein, a method of analyzing the structure of the target protein, a sustained-release formulation, and a method of producing an antibody against the target protein. The chaperonin-target protein complex in the present invention includes a fusion protein having a chaperonin subunit and an affinity tag linked to the chaperonin subunit via a peptide bond and a target protein for which the affinity tag shows a specific affinity, wherein the target protein is bound to the affinity tag by means of the specific affinity, thereby forming a chaperonin ring structure consisting of a plurality of chaperonin subunits. The chaperonin-target protein in the present invention stabilizes the target protein and surely immobilize on a carrier without causing any change in its stereostructure.

    摘要翻译： 本发明提供了伴侣伴侣 - 靶蛋白复合物及其制造方法，使靶蛋白稳定化的方法，固定靶蛋白的方法，分析靶蛋白的结构的方法，持续释放 制剂和产生针对靶蛋白的抗体的方法。 本发明中的伴侣伴侣 - 靶蛋白复合物包括伴侣伴侣亚基和通过肽键与伴侣蛋白亚基连接的亲和标签的融合蛋白和亲和标签显示特异性亲和力的靶蛋白，其中靶蛋白为 通过特异性亲和力结合亲和标签，从而形成由多个伴侣蛋白亚基组成的伴侣蛋白环结构。 本发明中的伴侣伴侣靶蛋白稳定靶蛋白，并确定地固定在载体上，而不引起其立体结构的任何改变。

    Process for production of a recombinant protein and a fusion protein
    4.
    发明授权
    Process for production of a recombinant protein and a fusion protein 失效
    生产重组蛋白和融合蛋白的方法

    公开(公告)号：US07608424B2

    公开(公告)日：2009-10-27

    申请号：US11837795

    申请日：2007-08-13

    申请人： Masahiro Furutani ,  Junichi Hata ,  Akiko Togi

    发明人： Masahiro Furutani ,  Junichi Hata ,  Akiko Togi

    IPC分类号： C12P21/04

    CPC分类号： C12N15/62 ,  C07K19/00 ,  C07K2319/21 ,  C07K2319/35 ,  C07K2319/40 ,  C07K2319/75 ,  C12N15/70 ,  C12P21/02

    摘要： This invention provides a recombinant protein expression system using a host and cell-free translation system, and is capable of universally expressing a large amount of any protein as soluble protein, while preventing toxicity in hosts, formation of inclusion bodies, and decompositions with proteases. Such may be achieved by expressing the desired protein as a fusion protein with chaperoning, such as about 60 kDa molecular chaperons, 60 kDa heat shock proteins, or thermosomes, and accommodating the desired protein inside of a stereostructure of a chaperonin. The present invention provides a process for producing a protein, which comprises transcribing and translating a gene containing a gene encoding the linked chaperonin subunits and a gene encoding a desired protein thereby synthesizing a fusion protein having the desired protein linked via a peptide linkage to the linked chaperonin subunits.

    摘要翻译： 本发明提供了使用宿主和无细胞翻译系统的重组蛋白质表达系统，并且能够普遍表达大量的任何蛋白质作为可溶性蛋白质，同时防止宿主中的毒性，包涵体的形成和蛋白酶的分解。 这可以通过用伴侣蛋白表达所需蛋白质作为融合蛋白，例如约60kDa的分子伴侣，60kDa的热休克蛋白或者热单体，并且在伴侣蛋白的立体结构内容纳所需的蛋白质来实现。 本发明提供了一种生产蛋白质的方法，其包括转录和翻译含有编码连接的伴侣蛋白亚基的基因的基因和编码所需蛋白质的基因，从而合成具有通过肽键连接的所需蛋白质的融合蛋白与所连接的 伴侣亚单位。

    Process for production of a recombinant protein and a fusion protein
    5.
    发明授权
    Process for production of a recombinant protein and a fusion protein 有权
    生产重组蛋白和融合蛋白的方法

    公开(公告)号：US07276355B2

    公开(公告)日：2007-10-02

    申请号：US10451883

    申请日：2001-12-26

    申请人： Masahiro Furutani ,  Junichi Hata ,  Akiko Togi

    发明人： Masahiro Furutani ,  Junichi Hata ,  Akiko Togi

    IPC分类号： C12P21/04

    CPC分类号： C12N15/62 ,  C07K19/00 ,  C07K2319/21 ,  C07K2319/35 ,  C07K2319/40 ,  C07K2319/75 ,  C12N15/70 ,  C12P21/02

    摘要： This invention provides a recombinant protein expression system using a host and cell-free translation system, and is capable of universally expressing a large amount of any protein as soluble protein, while preventing toxicity in hosts, formation of inclusion bodies, and decomposition with proteases. Such may be achieved by expressing the desired protein as a fusion protein with chaperonins, such as about 60 kDa molecular chaperones, 60 kDa heat shock proteins, or thermosomes, and accommodating the desired protein inside of a stereostructure of a chaperonin. The present invention provides a process for producing a protein, which comprises transcribing and translating a gene containing a gene encoding the linked chaperonin subunits and a gene encoding a desired protein thereby synthesizing a fusion protein having the desired protein linked via a peptide linkage to the linked chaperonin subunits.

    摘要翻译： 本发明提供了使用宿主和无细胞翻译系统的重组蛋白质表达系统，并且能够普遍表达大量的任何蛋白质作为可溶性蛋白质，同时防止宿主中的毒性，包涵体的形成和蛋白酶的分解。 这可以通过用伴侣蛋白（例如约60kDa的分子伴侣，60kDa的热休克蛋白或热单体）表达所需的蛋白质作为融合蛋白，并且将伴侣蛋白的立体结构中的所需蛋白质容纳。 本发明提供了一种生产蛋白质的方法，其包括转录和翻译含有编码连接的伴侣蛋白亚基的基因的基因和编码所需蛋白质的基因，从而合成具有通过肽键连接的所需蛋白质的融合蛋白与所连接的 伴侣亚单位。

    Image photographing apparatus, method of controlling image photographing apparatus and control program
    6.
    发明授权
    Image photographing apparatus, method of controlling image photographing apparatus and control program 有权
    图像拍摄装置，控制图像拍摄装置的方法和控制程序

    公开(公告)号：US08537217B2

    公开(公告)日：2013-09-17

    申请号：US12695224

    申请日：2010-01-28

    申请人： Masahiro Furutani ,  Takashi Yamamoto ,  Kenji Ohmori

    发明人： Masahiro Furutani ,  Takashi Yamamoto ,  Kenji Ohmori

    IPC分类号： H04N7/18

    CPC分类号： G03B15/00 ,  G09G3/001 ,  G09G2340/04 ,  G09G2340/14 ,  G09G2354/00

    摘要： An image photographing apparatus is provided, which includes a camera section having a zoom function for changing a photographing field angle, a transmissive display section including a display surface that allows sides facing each other to be transmissively viewed from both directions of a housing front side and a housing back side, a distance detection section that detects a distance from a point of view of a photographer to the display surface of the display section, and a control section. The control section controls the display surface of the transmissive display section in a transmissively viewable state when in photographing mode so as to perform photographing by the camera section, and determines a photographing field angle using the zoom function of the camera section in response to the distance detected by the distance detection section.

    摘要翻译： 提供了一种图像拍摄装置，其包括具有用于改变拍摄场角的缩放功能的相机部分，透射显示部分，其包括允许彼此相对的侧面从壳体前侧的两个方向透射地观察的显示表面;以及 检测从摄影者到显示部的显示面的距离的距离检测部，以及控制部。 控制部分在拍摄模式下以可透视的状态控制透射显示部分的显示表面，以便执行照相机部分的拍摄，并且响应于距离来确定摄像视场角度，使用照相机部分的变焦功能 由距离检测部检测。

    Anti-human CCR7 antibody, hybridoma, nucleic acid, vector, cell, pharmaceutical composition, and antibody-immobilized carrier
    7.
    发明授权
    Anti-human CCR7 antibody, hybridoma, nucleic acid, vector, cell, pharmaceutical composition, and antibody-immobilized carrier 有权
    抗人CCR7抗体，杂交瘤，核酸，载体，细胞，药物组合物和抗体固定载体

    公开(公告)号：US08865170B2

    公开(公告)日：2014-10-21

    申请号：US13876265

    申请日：2011-09-27

    申请人： Naoki Nishiguchi ,  Akiyoshi Hirayama ,  Masahiro Furutani ,  Tatsuo Shimizu ,  Kiyoshi Takayama ,  Tomoko Shimizu ,  Kazuya Suzuki

    发明人： Naoki Nishiguchi ,  Akiyoshi Hirayama ,  Masahiro Furutani ,  Tatsuo Shimizu ,  Kiyoshi Takayama ,  Tomoko Shimizu ,  Kazuya Suzuki

    IPC分类号： C07K16/28 ,  A61K31/713 ,  A61K39/00

    CPC分类号： C07K16/2866 ,  A61K31/713 ,  A61K2039/505 ,  C07K2317/70 ,  C07K2317/76

    摘要： The present invention is novel anti-human-CC-motif-receptor-7 (anti-human-CCR7) antibodies useful for treating tissue fibrosis or cancer, and pharmaceutical compositions containing the anti-human-CCR7 antibodies. The invention includes an anti-human-CCR7 antibody specifically binding to an extracellular domain of human CCR7, having a heavy chain CDR3 containing an amino acid sequence represented by SEQ ID NO: 7, SEQ ID NO: 17, SEQ ID NO: 27, SEQ ID NO: 37, SEQ ID NO: 47, SEQ ID NO: 57, SEQ ID NO: 67, or SEQ ID NO: 77. The invention also includes an anti-human-CCR7 antibody having heavy chain CDRs 1-3 and light chain CDRs 1-3 containing amino acid sequences represented by SEQ ID NOs: 5-10, 15-20, 25-30, 35-40, 45-50, 55-60, 65-70, or 75-80. Preferably, the antibody has an activity of interfering with a CCR7-dependent intracellular signal transduction mechanism caused by CCR7 ligand stimulation.

    摘要翻译： 本发明是可用于治疗组织纤维化或癌症的新型抗人CC-基质受体-7（抗人CCR7）抗体，以及含有抗人CCR7抗体的药物组合物。 本发明包括与人CCR7的细胞外结构域特异性结合的抗人CCR7抗体，其具有含有SEQ ID NO：7，SEQ ID NO：17，SEQ ID NO：27所示氨基酸序列的重链CDR3， SEQ ID NO：37，SEQ ID NO：47，SEQ ID NO：57，SEQ ID NO：67或SEQ ID NO：77.本发明还包括具有重链CDR 1-3的抗人CCR7抗体， 含有由SEQ ID NO：5-10，15-20,25-30,35-40,45-50,55-60,65-70或75-80表示的氨基酸序列的轻链CDR1-3。 优选地，抗体具有干扰由CCR7配体刺激引起的CCR7依赖性细胞内信号转导机制的活性。

    Expression vector, host, fused protein, process for producing fused protein and process for producing protein
    10.
    发明申请
    Expression vector, host, fused protein, process for producing fused protein and process for producing protein 审中-公开
    表达载体，宿主，融合蛋白，产生融合蛋白的方法和生产蛋白质的方法

    公开(公告)号：US20050130259A1

    公开(公告)日：2005-06-16

    申请号：US10511098

    申请日：2003-06-25

    申请人： Akira Ideno ,  Tadashi Maruyama ,  Masahiro Furutani

    发明人： Akira Ideno ,  Tadashi Maruyama ,  Masahiro Furutani

    IPC分类号： C07K19/00 ,  C12N1/21 ,  C12N15/62 ,  C12N15/70 ,  C12P21/02 ,  C07H21/04 ,  C12N9/16 ,  C12P21/04 ,  C12P21/06

    CPC分类号： C12N15/70 ,  C07K2319/35 ,  C07K2319/50 ,  C12N15/62 ,  C12P21/02

    摘要： It is an object of the present invention to provide an expression vector, a host, a fused protein, a protein, a process for producing a fused protein, and a process for producing a protein, which can prevent formation of an unactive abnormal protein at production of a recombinant protein, and can produce a desired protein as a natural type, that is, a soluble type at a large amount and effectively. That is, the present invention is an expression vector, which comprises: (a) a first coding region encoding a polypeptide having molecular chaperone activity, and (b) a region having at least one restriction enzyme site in which a second coding region encoding a protein can be inserted. In the expression vector of the present invention, the first coding region is operatively linked to a promoter, and the restriction enzyme sites are in the same reading frame as the first coding region, and are downstream of the first coding region, or the restriction enzyme sites are disposed so that the inserted second coding region is operatively linked to a promoter, and the first coding region is in the same reading frame as the second coding region, and is downstream of the second coding region.

    摘要翻译： 本发明的目的是提供表达载体，宿主，融合蛋白，蛋白质，产生融合蛋白的方法以及制备蛋白质的方法，其可以防止非活性异常蛋白质的形成 生产重组蛋白质，并且可以以大量和有效地产生期望的天然蛋白质，即可溶型。 也就是说，本发明是一种表达载体，其包括：（a）编码具有分子伴侣活性的多肽的第一编码区，和（b）具有至少一个限制酶位点的区域，其中编码 蛋白质可以插入。 在本发明的表达载体中，第一编码区与启动子可操作地连接，限制酶位点与第一编码区位于相同的阅读框中，并且位于第一编码区的下游，或限制酶 布置位置使得插入的第二编码区域可操作地连接到启动器，并且第一编码区域与第二编码区域处于相同的读取框中，并且在第二编码区域的下游。