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公开(公告)号:US08301388B2
公开(公告)日:2012-10-30
申请号:US10841375
申请日:2004-05-05
申请人: Keith E. Stormo , QuanZhou Tao , Robert Bogden
发明人: Keith E. Stormo , QuanZhou Tao , Robert Bogden
IPC分类号: G01N33/50
CPC分类号: C40B30/04 , C12N15/1034
摘要: This application pertains to construction of pooled biological material such as DNA, RNA, proteins and the like that are able to be screened by a wide variety of methods such as PCR (Polymerase Chain Reaction), DNA/DNA hybridization, DNA/RNA hybridization, RNA/RNA hybridization, single strand DNA probing, protein/protein hybridization and a wide variety of additional methods. Our new method for construction of pools and superpools for screening differs in that the complete set is systematically divided into a variety of smaller subsets which are then re-pooled to make the final screening pools. This pooled material can be from individual samples or a population of samples. In order to reduce the analysis time, materials and expense, the pooling of high resolution small pools in a matrix allows for a lower number of user experiments to have higher resolution (as if the researcher had analyzed the complete set of small pools).
摘要翻译: 本申请涉及能够通过PCR(聚合酶链式反应),DNA / DNA杂交,DNA / RNA杂交等各种方法进行筛选的合成生物材料如DNA,RNA,蛋白质等的构建, RNA / RNA杂交,单链DNA探测,蛋白质/蛋白质杂交和各种其他方法。 我们用于筛选池和超级泳池的新方法不同之处在于,将整套系统分为多个较小的子集,然后将其重新集中以进行最终筛选池。 这种汇集的材料可以来自个别样品或一组样品。 为了减少分析时间,材料和费用,矩阵中的高分辨率小池的集合允许较低数量的用户实验具有更高的分辨率(就好像研究人员已经分析了一整套小池)。