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    Pool and superpool matrix coding and decoding designs and methods
    1.
    发明授权
    Pool and superpool matrix coding and decoding designs and methods 有权
    池和超级矩阵编码和解码设计和方法

    公开(公告)号：US08301388B2

    公开(公告)日：2012-10-30

    申请号：US10841375

    申请日：2004-05-05

    申请人： Keith E. Stormo ,  QuanZhou Tao ,  Robert Bogden

    发明人： Keith E. Stormo ,  QuanZhou Tao ,  Robert Bogden

    IPC分类号： G01N33/50

    CPC分类号： C40B30/04 ,  C12N15/1034

    摘要： This application pertains to construction of pooled biological material such as DNA, RNA, proteins and the like that are able to be screened by a wide variety of methods such as PCR (Polymerase Chain Reaction), DNA/DNA hybridization, DNA/RNA hybridization, RNA/RNA hybridization, single strand DNA probing, protein/protein hybridization and a wide variety of additional methods. Our new method for construction of pools and superpools for screening differs in that the complete set is systematically divided into a variety of smaller subsets which are then re-pooled to make the final screening pools. This pooled material can be from individual samples or a population of samples. In order to reduce the analysis time, materials and expense, the pooling of high resolution small pools in a matrix allows for a lower number of user experiments to have higher resolution (as if the researcher had analyzed the complete set of small pools).

    摘要翻译： 本申请涉及能够通过PCR（聚合酶链式反应），DNA / DNA杂交，DNA / RNA杂交等各种方法进行筛选的合成生物材料如DNA，RNA，蛋白质等的构建， RNA / RNA杂交，单链DNA探测，蛋白质/蛋白质杂交和各种其他方法。 我们用于筛选池和超级泳池的新方法不同之处在于，将整套系统分为多个较小的子集，然后将其重新集中以进行最终筛选池。 这种汇集的材料可以来自个别样品或一组样品。 为了减少分析时间，材料和费用，矩阵中的高分辨率小池的集合允许较低数量的用户实验具有更高的分辨率（就好像研究人员已经分析了一整套小池）。

    Microbial remediation reactor and process
    3.
    发明授权
    Microbial remediation reactor and process 失效
    微生物修复反应器及工艺

    公开(公告)号：US06346412B1

    公开(公告)日：2002-02-12

    申请号：US09254309

    申请日：1999-03-03

    申请人： Keith E. Stormo

    发明人： Keith E. Stormo

    IPC分类号： C12S1300

    CPC分类号： C02F3/1284 ,  B01F3/04539 ,  B01F3/04836 ,  B01F3/08 ,  B01F3/0857 ,  B01F5/0218 ,  B01F5/0231 ,  B01F7/00291 ,  B01F7/00633 ,  B01F7/00641 ,  B01F15/005 ,  B01F2003/04546 ,  B01F2003/04567 ,  B01F2003/04687 ,  B09C1/10 ,  C12M21/04 ,  C12M27/04 ,  C12M29/02 ,  C12M29/24 ,  Y02W10/15

    摘要： Embodiments of a remediation reactor and mixer/contactor blade for the reactor are shown and described, the reactor (11) being for containing a liquid slurry, suspension or settled bed of solid particles containing microorganisms. Inside the vessel is a supply conduit and at least one generally horizontal stirrer blade (27) in fluid connection with the supply conduit. The stirrer blade is a mixer/contactor which has a leading side having openings through which fluid may pass. The stirrer blade rotates in the vessel, and this rotation is made easier by the hydraulic forces of fluid flowing out from the stirrer blade. The flowing fluid creates a fluidization zone in the slurry, suspension or settled bed at or near the leading edge of the stirrer blade. The fluidization zone is less dense than the rest of the sediment bed, and the stirrer blade tends to rotate into the fluidization zone. This way, controlled rotation of the stirrer blade may be created near the bottom of the vessel, enhancing mixing of the microorganisms with the slurry, suspension or settled bed in the vessel, without unnecessarily damaging the microorganisms, especially when the flowing fluid contains contaminants which are nutrients for the microorganisms.

    摘要翻译： 示出并描述了用于反应器的修复反应器和混合器/接触器叶片的实施例，反应器（11）用于容纳含有微生物的固体颗粒的液体浆料，悬浮液或沉降床。 在容器内部是供应管道和至少一个与供应导管流体连接的大致水平的搅拌器叶片（27）。 搅拌器叶片是混合器/接触器，其具有带有开口的前端，流体可通过该开口。 搅拌器叶片在容器中旋转，并且通过从搅拌器叶片流出的流体的液压力使得该旋转变得更容易。 流动的流体在浆液，悬浮液或沉降床中在搅拌器叶片的前缘或附近产生流化区。 流化区比沉积床的其余部分密度小，搅拌叶片倾向于旋转进入流化区。 这样，可以在容器的底部附近产生搅拌器叶片的受控旋转，增强微生物与容器中的浆料，悬浮液或沉降床的混合，而不会不必要地损害微生物，特别是当流动的流体含有污染物时 是微生物的养分。

    Slurry reactor
    4.
    发明授权
    Slurry reactor 失效
    泥浆反应器

    公开(公告)号：US5616304A

    公开(公告)日：1997-04-01

    申请号：US426566

    申请日：1995-04-21

    申请人： Keith E. Stormo

    发明人： Keith E. Stormo

    IPC分类号： B01F3/04 ,  B01F5/02 ,  B01F5/10 ,  B01F7/00 ,  B01F15/00 ,  B01J8/10 ,  B01J8/22 ,  B01J19/18 ,  C02F3/06 ,  C02F3/12 ,  C02F3/28 ,  C12M1/06 ,  B01F13/02 ,  B01F7/20

    CPC分类号： C02F3/1284 ,  B01F15/00525 ,  B01F3/04531 ,  B01F5/0218 ,  B01F5/0231 ,  B01F5/106 ,  B01F7/00916 ,  B01J19/18 ,  B01J8/10 ,  B01J8/222 ,  C02F3/06 ,  C02F3/28 ,  C12M21/04 ,  C12M27/04 ,  C12M29/02 ,  C12M29/24 ,  B01F15/005 ,  B01F2003/04702 ,  B01J2219/00162 ,  B01J2219/00164 ,  B01J2219/00189 ,  Y02W10/15

    摘要： Embodiments of a slurry reactor and slurry mixer/contactor are shown and described. Each embodiment of the reactor includes a vessel for containing a liquid slurry or suspension of particles and microorganisms. In the inside of the vessel, generally along its centerline, is a vertical conduit extending from near the top to near the bottom. At the bottom of the vertical conduit is at least one generally horizontal, hollow, stirrer blade in fluid connection with the vertical conduit. The stirrer blade is a mixer/contactor which has openings near its leading edge so fluid may pass therethrough. The stirrer blade may rotate around the vertical conduit if the conduit is fixed, or the conduit, with the stirrer blade fixed to it, may rotate around in the vessel. In any event, the rotation of the stirrer blade is caused by the hydraulic forces of fluid flowing out from the stirrer blade. The flowing fluid creates a fluidization zone in the slurry sediment bed at the leading edge of the stirrer. The fluidization zone is less dense than the rest of the sediment bed, and the stirrer blade tends to rotate into the fluidization zone. This way, controlled rotation of the stirrer blade may be created near the bottom of the vessel, enhancing mixing of the microorganisms with the slurry or suspension in the vessel, without unnecessarily damaging the microorganisms.

    摘要翻译： 示出并描述了浆料反应器和浆料混合器/接触器的实施方案。 反应器的每个实施例包括用于容纳液体浆料或颗粒和微生物悬浮液的容器。 在船舶的内部，一般沿其中心线是从顶部附近延伸到靠近底部的垂直管道。 在垂直导管的底部是至少一个与垂直导管流体连接的大致水平的，中空的搅拌器叶片。 搅拌器叶片是在其前缘附近具有开口的混合器/接触器，因此流体可以通过。 如果导管被固定，则搅拌器叶片可围绕垂直导管旋转，或者将搅拌器叶片固定在其上的导管可在容器内旋转。 在任何情况下，搅拌器叶片的旋转是由从搅拌叶片流出的流体的液压力引起的。 流动的流体在搅拌器前缘的泥浆沉积床中产生流化区。 流化区比沉积床的其余部分密度小，搅拌叶片倾向于旋转进入流化区。 这样，可以在容器的底部附近产生搅拌器叶片的受控旋转，增强微生物与容器中浆液或悬浮液的混合，而不会不必要地损害微生物。

    Dynamic bed reactor
    5.
    发明授权
    Dynamic bed reactor 失效
    动态床反应器

    公开(公告)号：US5531897A

    公开(公告)日：1996-07-02

    申请号：US960963

    申请日：1992-10-13

    申请人： Keith E. Stormo

    发明人： Keith E. Stormo

    IPC分类号： C02F3/06 ,  C02F3/10 ,  C12M1/36 ,  C12M1/40 ,  C12N11/00 ,  C12N11/04 ,  C12N11/08 ,  C02F3/00 ,  C02F1/28

    CPC分类号： C12M25/14 ,  C02F3/06 ,  C02F3/10 ,  C12M27/00 ,  C12M41/26 ,  C12M41/32 ,  C12N11/00 ,  C12N11/04 ,  C12N11/08 ,  Y02W10/15

    摘要： A dynamic bed reactor is disclosed in which a compressible open cell foam matrix is periodically compressed and expanded to move a liquid or fluid through the matrix. In preferred embodiments, the matrix contains an active material such as an enzyme, biological cell, chelating agent, oligonucleotide, adsorbent or other material that acts upon the liquid or fluid passing through the matrix. The active material may be physically immobilized in the matrix, or attached by covalent or ionic bonds. Microbeads, substantially all of which have diameters less than 50 microns, can be used to immobilize the active material in the matrix and further improve reactor efficiency. A particularly preferred matrix is made of open cell polyurethane foam, which adsorbs pollutants such as polychlorophenol or o-nitrophenol. The reactors of the present invention allow unidirectional non-laminar flow through the matrix, and promote intimate exposure of liquid reactants to active agents such as microorganisms immobilized in the matrix.

    摘要翻译： 公开了一种动态床反应器，其中可压缩开孔泡沫基质被周期性地压缩和膨胀以将液体或流体移动通过基质。 在优选的实施方案中，基质含有活性物质，例如酶，生物细胞，螯合剂，寡核苷酸，吸附剂或作用于通过基质的液体或流体的其它材料。 活性物质可以物理固定在基质中，或通过共价或离子键连接。 基本上所有直径小于50微米的微珠可用于将活性材料固定在基质中，并进一步提高反应器效率。 特别优选的基质由开孔聚氨酯泡沫制成，其吸附诸如聚氯酚或邻硝基苯酚的污染物。 本发明的反应器允许通过基质的单向非层流，并促进液体反应物对活性剂如固定在基质中的微生物的亲和暴露。