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1.发明授权Analytical instrument having improved arrangement of reagent section and analytical method 有权具有改进的试剂部分布置和分析方法的分析仪器公开(公告)号：US07780828B2
公开(公告)日：2010-08-24
申请号：US10585295
申请日：2005-01-07
IPC分类号： G01N27/327
CPC分类号： C12Q1/006
摘要： The present invention relates to an analytical instrument (1) which includes a flow path (5) for moving a sample containing blood cells, an introduction port (50) for introducing the sample into the flow path (5), a reagent portion (51) arranged in the flow path (5), and an electron detection medium (52) for obtaining information necessary for analyzing an analysis target component contained in the sample in relation with an amount of electrons transferred. The reagent portion (51) contains an electron mediator for supplying an electron taken from the analysis target component in the sample to the electron detection medium (52), and at least part of the reagent portion is positioned adjacent to the introduction port (50).
摘要翻译： 本发明涉及一种分析仪器（1），其包括用于移动含有血细胞的样品的流路（5），用于将样品引入流路（5）的引入口（50），试剂部分 ）和电子检测介质（52），用于获得与所传送的电子数量相关的用于分析样品中包含的分析对象成分所需的信息。 试剂部（51）包含用于将从样品中的分析对象成分获取的电子供给到电子检测介质（52）的电子介体，并且所述试剂部的至少一部分位于与所述引入口（50）相邻的位置， 。
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2.再颁专利公开(公告)号：USRE44522E1
公开(公告)日：2013-10-08
申请号：US12822869
申请日：2010-06-24
申请人： Kenji Nagakawa , Hideaki Yamaoka
发明人： Kenji Nagakawa , Hideaki Yamaoka
IPC分类号： G01N27/416
摘要： The present invention relates to technology for constructing a reaction system including a test target, an oxidation-reduction enzyme, and an electron mediator, and measuring the concentration of the test target by an electrochemical process. A Ru compound is used as the electron mediator. The present invention provides a concentration test instrument including a substrate, first and second electrodes formed on the substrate, and a reagent layer formed as a solid. The reagent layer contains an oxidation-reduction enzyme and a Ru compound, and is constituted so as to dissolve and construct a liquid phase reaction system when a sample liquid is supplied.
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3.发明授权公开(公告)号：US07390391B2
公开(公告)日：2008-06-24
申请号：US10489488
申请日：2002-08-30
申请人： Kenji Nagakawa , Hideaki Yamaoka
发明人： Kenji Nagakawa , Hideaki Yamaoka
IPC分类号： G01N27/416
CPC分类号： C12Q1/004
摘要： The present invention relates to technology for constructing a reaction system including a test target, an oxidation-reduction enzyme, and an electron mediator, and measuring the concentration of the test target by an electrochemical process. A Ru compound is used as the electron mediator. The present invention provides a concentration test instrument including a substrate, first and second electrodes formed on the substrate, and a reagent layer formed as a solid. The reagent layer contains an oxidation-reduction enzyme and a Ru compound, and is constituted so as to dissolve and construct a liquid phase reaction system when a sample liquid is supplied.
摘要翻译： 本发明涉及用于构建包括测试对象，氧化还原酶和电子介体的反应系统的技术，并且通过电化学过程测量测试目标的浓度。 使用Ru化合物作为电子介体。 本发明提供了一种浓度测试仪器，其包括基板，形成在基板上的第一和第二电极以及形成为固体的试剂层。 试剂层含有氧化还原酶和Ru化合物，构成为在供给样品液时溶解并构成液相反应体系。
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4.发明申请Analytical instrument having improved arrangement of reagent section and analytical method 有权具有改进的试剂部分布置和分析方法的分析仪器公开(公告)号：US20090017483A1
公开(公告)日：2009-01-15
申请号：US10585295
申请日：2005-01-07
CPC分类号： C12Q1/006
摘要： The present invention relates to an analytical instrument (1) which includes a flow path (5) for moving a sample containing blood cells, an introduction port (50) for introducing the sample into the flow path (5), a reagent portion (51) arranged in the flow path (5), and an electron detection medium (52) for obtaining information necessary for analyzing an analysis target component contained in the sample in relation with an amount of electrons transferred. The reagent portion (51) contains an electron mediator for supplying an electron taken from the analysis target component in the sample to the electron detection medium (52), and at least part of the reagent portion is positioned adjacent to the introduction port (50).
摘要翻译： 本发明涉及一种分析仪器（1），其包括用于移动含有血细胞的样品的流路（5），用于将样品引入流路（5）的引入口（50），试剂部分 ）和电子检测介质（52），用于获得与所传送的电子数量相关的用于分析样品中包含的分析对象成分所需的信息。 试剂部（51）包含用于将从样品中的分析对象成分获取的电子供给到电子检测介质（52）的电子介体，并且所述试剂部的至少一部分位于与所述引入口（50）相邻的位置， 。
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公开(公告)号：US20110076707A1
公开(公告)日：2011-03-31
申请号：US12861734
申请日：2010-08-23
申请人： Hideaki Yamaoka
发明人： Hideaki Yamaoka
CPC分类号： C12N9/0006 , C12Q1/32
摘要： Substrate specificity for glucose of a glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 13 is improved by substituting another amino acid residue for the amino acid residue at position 472 and/or 475.
摘要翻译： 通过用另一个氨基酸残基取代位置472和/或475处的氨基酸残基来提高具有SEQ ID NO：13氨基酸序列的葡萄糖脱氢酶对葡萄糖的葡萄糖的底物特异性。
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公开(公告)号：US20060042361A1
公开(公告)日：2006-03-02
申请号：US10533602
申请日：2003-10-30
申请人： Hideaki Yamaoka , Koji Katsuki
发明人： Hideaki Yamaoka , Koji Katsuki
IPC分类号： G01N1/40
CPC分类号： C12Q1/006 , C12Q1/001 , C12Q1/005 , G01N27/3272
摘要： The present invention relates to a measuring instrument (1) comprising a channel (60) for moving a sample liquid (BL) containing a solid component (B1) and providing a liquid reaction field and first and second electrodes (31, 32) which are used to apply voltage to the liquid reaction field. The first electrode (31) has an electron transfer interface (31a) for transferring electrons between it and the liquid reaction field when voltage is applied to the liquid reaction field via the first and second electrodes (31, 32). The measuring instrument (1) comprises concentrating means (51) for increasing the concentration of solid components at portions thereof which contact the electron transfer interface (31a) in the liquid reaction field. The concentrating means (51) preferably comprises a water-absorbing layer containing an absorbent polymer material.
摘要翻译： 本发明涉及一种测量仪器（1），其包括用于移动含有固体组分（B 1）并提供液体反应场的样品液体（BL）的通道（60）和第一和第二电极（31,32），所述第一和第二电极 用于向液体反应场施加电压。 当电压通过第一和第二电极（31,32）施加到液体反应场时，第一电极（31）具有用于在其与液体反应场之间转移电子的电子转移界面（31a）。 测量仪器（1）包括浓缩装置（51），用于增加在液体反应场中接触电子传递界面（31a）的部分处的固体组分的浓度。 浓缩装置（51）优选包括含有吸收性聚合物材料的吸水层。
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7.发明申请Glucose level measuring method and glucose sensor utilizing glucose dehydrogenase 有权使用葡萄糖脱氢酶的葡萄糖水平测量方法和葡萄糖传感器公开(公告)号：US20080131919A1
公开(公告)日：2008-06-05
申请号：US12008358
申请日：2008-01-10
IPC分类号： C12Q1/54 , G01N27/327
CPC分类号： G01N27/3272 , C12Q1/004 , C12Q1/006 , C12Q1/32
摘要： The present invention relates to a technique for measuring a glucose level by utilizing a reaction system containing an enzyme and an electron carrier. In accordance with the glucose level measuring method of the present invention, glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier. The present invention further provides a glucose sensor in which glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier.
摘要翻译： 本发明涉及利用含有酶和电子载体的反应体系来测量葡萄糖水平的技术。 根据本发明的葡萄糖水平测定方法，使用附着有细胞色素C的葡萄糖脱氢酶或来自属于伯克霍尔德氏菌属的微生物的葡萄糖脱氢酶作为酶，使用Ru化合物作为电子载体。 本发明还提供一种葡萄糖传感器，其中使用连接有细胞色素C的葡萄糖脱氢酶或衍生自属于伯克霍尔德氏菌属的微生物的葡萄糖脱氢酶作为酶，并且使用Ru化合物作为电子载体。
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公开(公告)号：US07201042B2
公开(公告)日：2007-04-10
申请号：US10533602
申请日：2003-10-30
申请人： Hideaki Yamaoka , Koji Katsuki
发明人： Hideaki Yamaoka , Koji Katsuki
CPC分类号： C12Q1/006 , C12Q1/001 , C12Q1/005 , G01N27/3272
摘要： The present invention relates to a measuring instrument (1) comprising a channel (60) for moving a sample liquid (BL) containing a solid component (B1) and providing a liquid reaction field and first and second electrodes (31, 32) which are used to apply voltage to the liquid reaction field. The first electrode (31) has an electron transfer interface (31a) for transferring electrons between it and the liquid reaction field when voltage is applied to the liquid reaction field via the first and second electrodes (31, 32). The measuring instrument (1) comprises concentrating means (51) for increasing the concentration of solid components at portions thereof which contact the electron transfer interface (31a) in the liquid reaction field. The concentrating means (51) preferably comprises a water-absorbing layer containing an absorbent polymer material.
摘要翻译： 本发明涉及一种测量仪器（1），包括用于移动含有固体组分（B 1）并提供液体反应场的样品液体（BL）的通道（60）和第一和第二电极（31,32），所述第一和第二电极 用于向液体反应场施加电压。 当电压通过第一和第二电极（31,32）施加到液体反应场时，第一电极（31）具有用于在其和液体反应场之间转移电子的电子转移界面（31a）。 测量仪器（1）包括浓缩装置（51），用于增加在液体反应场中接触电子传递界面（31a）的部分处的固体组分的浓度。 浓缩装置（51）优选包括含有吸收性聚合物材料的吸水层。
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公开(公告)号：US20060094098A1
公开(公告)日：2006-05-04
申请号：US10526026
申请日：2003-08-20
申请人： Hideaki Yamaoka , Keisuke Kurosaka , Shido Kawase
发明人： Hideaki Yamaoka , Keisuke Kurosaka , Shido Kawase
IPC分类号： C12N9/02
CPC分类号： C12N9/0006 , C07K1/18 , C07K1/20 , C12N9/0004
摘要： A method of purifying a target protein from a solution, in which the target protein containing an electron transfer protein is dissolved, with the use of liquid chromatography. The liquid chromatography is performed by introducing the above-described protein solution into a tank filled with a packing agent, thus bonding the target protein to the packing agent, removing impurities, and then eluting the target protein from the packing agent with the use of an eluent containing a hydroxycholanoic acid salt. As an example of the above protein, glucose dehydrogenase containing a protein having an activity of dehydrogenating glucose is cited. The liquid chromatography is performed by combining hydrophobic chromatography with anion exchange chromatography.
摘要翻译： 使用液相色谱从溶解了含有电子转移蛋白的靶蛋白质的溶液中提纯目标蛋白质的方法。 通过将上述蛋白质溶液导入填充有填充剂的槽中进行液相色谱法，从而将目标蛋白质与填充剂结合，除去杂质，然后使用包装剂从包装剂中洗脱目标蛋白质 洗脱液含有羟基胆酸盐。 作为上述蛋白质的例子，可以列举含有葡萄糖脱氢活性的蛋白质的葡萄糖脱氢酶。 通过将疏水层析与阴离子交换层析结合进行液相色谱。
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公开(公告)号：US08210349B2
公开(公告)日：2012-07-03
申请号：US10550287
申请日：2004-03-25
申请人： Hideaki Yamaoka
发明人： Hideaki Yamaoka
CPC分类号： G01N33/48778 , C12Q1/004
摘要： A sensor storage container is provided that allows sensors to be checked visually from the outside. A container is used for storing sensors including an oxidation-reduction enzyme that oxidizes or reduces an object to be analyzed, a mediator that mediates the transfer of electrons caused by oxidation or reduction, and a detection means that detects the oxidation or reduction reaction. The container is transparent or semi-transparent in whole or in part and thereby the sensors can be checked visually from the outside of the container. This container may be composed of a container body and a lid. The container body may be provided with a scale. Preferably, the sensors are those produced using a ruthenium metal complex as the mediator.
摘要翻译： 提供了一种传感器存储容器，其允许从外部视觉检查传感器。 容器用于存储氧化或还原被分析物的氧化还原酶的传感器，介导由氧化或还原引起的电子传递的介体以及检测氧化还原反应的检测装置。 容器全部或部分透明或半透明，从而可以从容器的外部视觉检查传感器。 该容器可以由容器主体和盖子组成。 容器主体可以设置有秤。 优选地，传感器是使用钌金属络合物作为介体产生的传感器。
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