公开(公告)号：US5126239A

公开(公告)日：1992-06-30

申请号：US494258

申请日：1990-03-14

申请人： Kenneth J. Livak ,  Jan A. Rafalski ,  Scott V. Tingey ,  John G. Williams

发明人： Kenneth J. Livak ,  Jan A. Rafalski ,  Scott V. Tingey ,  John G. Williams

IPC分类号： C12N15/09 ,  C12Q1/68

CPC分类号： C12Q1/6858 ,  Y10T436/143333

摘要： A process is provided for detecting polymorphisms on the basis of nucleotide differences in random segments of the nucleic acid by performing a primer extension reaction on the nucleic acids and comparing the extension reaction products. The random nucleic acid segment can be amplified by first dissociating the extension product from the template and contacting the dissociated extension product with a primer under conditions such that an amplification extension product is synthesized using the dissociated extension product as a template. Differences in the extension product are useful as markers in constructing genetic maps and as markers to distinguish or identify individuals.

摘要翻译： 提供了通过对核酸进行引物延伸反应并比较延伸反应产物来检测基于核酸随机区段的核苷酸差异的多态性的方法。 可以通过首先将延伸产物从模板中解离并使解离的延伸产物与引物接触并在使用解离的延伸产物作为模板合成扩增产物的条件下扩增随机核酸片段。 扩展产物的差异可用作构建遗传图谱的标记和用于区分或识别个体的标记。

公开(公告)号：US6037127A

公开(公告)日：2000-03-14

申请号：US979269

申请日：1997-11-26

申请人： Richard C. Ebersole ,  Edwin R. Hendrickson ,  Mark S. Payne ,  Sandra Fitzpatrick-McElligott ,  William R. Majarian ,  Jan A. Rafalski

发明人： Richard C. Ebersole ,  Edwin R. Hendrickson ,  Mark S. Payne ,  Sandra Fitzpatrick-McElligott ,  William R. Majarian ,  Jan A. Rafalski

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6816 ,  C12Q1/6834 ,  Y10T436/143333

摘要： A method for detecting the presence of a nucleic acid analyte in a test sample is provided in which a test sample is contacted with a test strip of a chromatographic bibulous porous material which is capable of moving the test sample laterally along the test strip by capillary migration to ultimate capture by a moiety in a specific capture zone.

摘要翻译： 提供了一种用于检测测试样品中核酸分析物的存在的方法，其中将测试样品与色谱吸水多孔材料的测试条接触，所述测试条能够通过毛细管迁移沿着测试条横向移动测试样品 以通过特定捕获区中的部分进行最终捕获。