公开(公告)号：US07738086B2

公开(公告)日：2010-06-15

申请号：US11800468

申请日：2007-05-04

申请人： Kenneth L. Shepard ,  Rastislav Levicky ,  George Patounakis

发明人： Kenneth L. Shepard ,  Rastislav Levicky ,  George Patounakis

IPC分类号： G01N21/00

CPC分类号： H04N5/37452 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6454 ,  H01L27/14621 ,  H01L27/14643

摘要： An active CMOS biosensor chip for fluorescent-based detection is provided that enables time-gated, time-resolved fluorescence spectroscopy. In one embodiment, analytes are loaded with fluorophores that are bound to probe molecules immobilized on the surface of the chip. Photodiodes and other circuitry in the chip are used to measure the fluorescent intensity of the fluorophore at different times. These measurements are then averaged to generate a representation of the transient fluorescent decay response unique to the fluorophores. In addition to its low-cost, compact form, the biosensor chip provides capabilities beyond those of macroscopic instrumentation by enabling time-gated operation for background rejection, easing requirements on optical filters, and by characterizing fluorescence lifetime, allowing for a more detailed characterization of fluorophore labels and their environment. The biosensor chip can be used for a variety of applications including biological, medical, in-the-field applications, and fluorescent lifetime imaging applications.

摘要翻译： 提供了用于基于荧光的检测的有源CMOS生物传感器芯片，其能够进行时间选择的时间分辨荧光光谱。 在一个实施方案中，分析物载有与固定在芯片表面上的探针分子结合的荧光团。 芯片中的光电二极管和其他电路用于在不同时间测量荧光团的荧光强度。 然后对这些测量进行平均以产生荧光团特有的瞬态荧光衰变响应的表示。 除了其低成本，紧凑的形式之外，生物传感器芯片通过实现背景抑制的时间门控操作，对光学滤波器的放宽要求以及通过表征荧光寿命来提供超出宏观仪器的功能，允许更详细的表征 荧光标签及其环境。 生物传感器芯片可用于各种应用，包括生物，医学，现场应用和荧光寿命成像应用。

公开(公告)号：US20080037008A1

公开(公告)日：2008-02-14

申请号：US11800468

申请日：2007-05-04

申请人： Kenneth Shepard ,  Rastislav Levicky ,  George Patounakis

发明人： Kenneth Shepard ,  Rastislav Levicky ,  George Patounakis

IPC分类号： G01N21/00 ,  H01L27/14

CPC分类号： H04N5/37452 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6454 ,  H01L27/14621 ,  H01L27/14643

摘要： An active CMOS biosensor chip for fluorescent-based detection is provided that enables time-gated, time-resolved fluorescence spectroscopy. In one embodiment, analytes are loaded with fluorophores that are bound to probe molecules immobilized on the surface of the chip. Photodiodes and other circuitry in the chip are used to measure the fluorescent intensity of the fluorophore at different times. These measurements are then averaged to generate a representation of the transient fluorescent decay response unique to the fluorophores. In addition to its low-cost, compact form, the biosensor chip provides capabilities beyond those of macroscopic instrumentation by enabling time-gated operation for background rejection, easing requirements on optical filters, and by characterizing fluorescence lifetime, allowing for a more detailed characterization of fluorophore labels and their environment. The biosensor chip can be used for a variety of applications including biological, medical, in-the-field applications, and fluorescent lifetime imaging applications.

摘要翻译： 提供了用于基于荧光的检测的有源CMOS生物传感器芯片，其能够进行时间选择的时间分辨荧光光谱。 在一个实施方案中，分析物载有与固定在芯片表面上的探针分子结合的荧光团。 芯片中的光电二极管和其他电路用于在不同时间测量荧光团的荧光强度。 然后对这些测量进行平均以产生荧光团特有的瞬态荧光衰变响应的表示。 除了其低成本，紧凑的形式之外，生物传感器芯片通过实现背景抑制的时间门控操作，对光学滤波器的放宽要求以及通过表征荧光寿命来提供超出宏观仪器的功能，允许更详细的表征 荧光标签及其环境。 生物传感器芯片可用于各种应用，包括生物，医学，现场应用和荧光寿命成像应用。

公开(公告)号：US20070097364A1

公开(公告)日：2007-05-03

申请号：US11431405

申请日：2006-05-09

申请人： Kenneth Shepard ,  Rastislav Levicky ,  George Patounakis

发明人： Kenneth Shepard ,  Rastislav Levicky ,  George Patounakis

IPC分类号： G01J3/30 ,  G01N21/64

CPC分类号： G01J3/4406 ,  G01N21/6428 ,  G01N21/6454

摘要： An active CMOS biosensor chip for fluorescent-based detection is provided that enables time-gated, time-resolved fluorescence spectroscopy. Analytes are loaded with fluorophores that are bound to probe molecules immobilized on the surface of the chip. Photodiodes and other circuitry in the chip are used to measure the fluorescent intensity of the fluorophore at different times. These measurements are then averaged to generate a representation of the transient fluorescent decay response unique to the fluorophores. In addition to its low-cost, compact form, the biosensor chip provides capabilities beyond those of macroscopic instrumentation by enabling time-gated operation for background rejection, easing requirements on optical filters, and by characterizing fluorescence lifetime, allowing for a more detailed characterization of fluorophore labels and their environment. The biosensor chip can be used for a variety of applications including biological, medical, in-the-field applications, and fluorescent lifetime imaging applications.

摘要翻译： 提供了用于基于荧光的检测的有源CMOS生物传感器芯片，其能够进行时间选择的时间分辨荧光光谱。 分析物载有与固定在芯片表面上的探针分子结合的荧光团。 芯片中的光电二极管和其他电路用于在不同时间测量荧光团的荧光强度。 然后对这些测量进行平均以产生荧光团特有的瞬态荧光衰变响应的表示。 除了其低成本，紧凑的形式之外，生物传感器芯片通过实现背景抑制的时间门控操作，对光学滤波器的放宽要求以及通过表征荧光寿命来提供超出宏观仪器的功能，允许更详细的表征 荧光标签及其环境。 生物传感器芯片可用于各种应用，包括生物，医学，现场应用和荧光寿命成像应用。