公开(公告)号：US20050003346A1

公开(公告)日：2005-01-06

申请号：US10823294

申请日：2004-04-12

申请人： Kent Voorhees ,  John Rees ,  John Wheeler ,  Angelo Madonna

发明人： Kent Voorhees ,  John Rees ,  John Wheeler ,  Angelo Madonna

IPC分类号： C12Q1/04 ,  G01N33/543 ,  G01N33/569 ,  C12Q1/70

CPC分类号： C12Q1/04 ,  G01N33/54326 ,  G01N33/56911

摘要： A method for detecting one or more target bacteria in a raw sample where: 1) bacteriophage(s) specific to each target bacterium are added to the raw sample, 2) the test sample is incubated, and 3) the test sample is tested for the presence of each phage in sufficient numbers to indicate the presence of the associated target bacteria in the raw sample. In one embodiment, each phage is initially added to the raw sample in concentrations below the detection limit of the final phage detection process. In another embodiment, the parent phages are tagged in such a way that they can be separated from the progeny phage prior to the detection process. Preferred phage detection processes are immunoassay methods utilizing antibodies that bind specifically to each phage. Antibodies can be used that bind to the protein capsid of the phage. Alternatively, the phage can by dissociated after the incubation process and the sample tested for the presence of individual capsid proteins or phage nucleic acids. The invention can be used to test target bacteria for antibiotic resistance.

摘要翻译： 一种用于检测原料样品中的一种或多种目标细菌的方法，其中：1）将每种靶细菌特异性的噬菌体加入到原始样品中，2）将测试样品孵育，并且3）测试样品 每个噬菌体的存在足够数量以指示原始样品中相关靶细菌的存在。 在一个实施方案中，每个噬菌体最初以低于最终噬菌体检测过程的检测限的浓度加入到原始样品中。 在另一个实施方案中，母体噬菌体的标记方式使得它们可以在检测过程之前与后代噬菌体分离。 优选的噬菌体检测方法是利用与每个噬菌体特异性结合的抗体的免疫测定方法。 可以使用结合噬菌体的蛋白质衣壳的抗体。 或者，噬菌体可以通过在孵育过程之后解离并且样品测试个体衣壳蛋白或噬菌体核酸的存在。 本发明可用于测试目标细菌的抗生素耐药性。

公开(公告)号：US20090246752A1

公开(公告)日：2009-10-01

申请号：US12346656

申请日：2008-12-30

申请人： Kent Voorhees ,  Jon Rees ,  John H. Wheeler ,  Angelo Madonna

发明人： Kent Voorhees ,  Jon Rees ,  John H. Wheeler ,  Angelo Madonna

IPC分类号： C12Q1/70

CPC分类号： C12Q1/04 ,  G01N33/54326 ,  G01N33/56911

摘要： A method for detecting one or more target bacteria in a raw sample where: 1) bacteriophage(s) specific to each target bacterium are added to the raw sample, 2) the test sample is incubated, and 3) the test sample is tested for the presence of each phage in sufficient numbers to indicate the presence of the associated target bacteria in the raw sample. In one embodiment, each phage is initially added to the raw sample in concentrations below the detection limit of the final phage detection process. In another embodiment, the parent phages are tagged in such a way that they can be separated from the progeny phage prior to the detection process. Preferred phage detection processes are immunoassay methods utilizing antibodies that bind specifically to each phage. Antibodies can be used that bind to the protein capsid of the phage. Alternatively, the phage can by dissociated after the incubation process and the sample tested for the presence of individual capsid proteins or phage nucleic acids. The invention can be used to test target bacteria for antibiotic resistance.

摘要翻译： 一种用于检测原料样品中的一种或多种目标细菌的方法，其中：1）将每种靶细菌特异性的噬菌体加入到原始样品中，2）将测试样品孵育，并且3）测试样品 每个噬菌体的存在足够数量以指示原始样品中相关靶细菌的存在。 在一个实施方案中，每个噬菌体最初以低于最终噬菌体检测过程的检测限的浓度加入到原始样品中。 在另一个实施方案中，母体噬菌体的标记方式使得它们可以在检测过程之前与后代噬菌体分离。 优选的噬菌体检测方法是利用与每个噬菌体特异性结合的抗体的免疫测定方法。 可以使用结合噬菌体的蛋白质衣壳的抗体。 或者，噬菌体可以通过在孵育过程之后解离并且样品测试个体衣壳蛋白或噬菌体核酸的存在。 本发明可用于测试目标细菌的抗生素耐药性。

公开(公告)号：US20070275370A1

公开(公告)日：2007-11-29

申请号：US11626326

申请日：2007-01-23

申请人： Angelo Madonna ,  Kent Voorhees ,  Jon Rees

发明人： Angelo Madonna ,  Kent Voorhees ,  Jon Rees

IPC分类号： C12Q1/70

CPC分类号： C12Q1/04 ,  G01N33/54326 ,  G01N33/56911

摘要： The invention is directed to a method for detecting low concentrations of bacteria in liquid solution that may or may not be complex liquid solutions. In one embodiment, immunomagnetic separation (IMS) is used to separate target bacterium that may be in a liquid mixture from other constituents in the mixture. A low concentration of a bacteriophage for the target bacteria is subsequently used to infect target bacterial cells that have been captured using the IMS technique. If at least a certain concentration of target bacterium are present, the bacteriophage will multiply to a point that is detectable. Matrix assisted laser desorption ionization/time-of-flight-mass spectrometry (MALDI/TOF-MS) is then used to produce a mass spectrum that is analyzed to determine if one or more proteins associated with the bacteriophage are present, thereby indirectly indicating that target bacterium were present in the liquid mixture.

摘要翻译： 本发明涉及一种用于检测液体溶液中低浓度细菌的方法，该方法可能是或可能不是复杂的液体溶液。 在一个实施方案中，使用免疫磁性分离（IMS）来分离来自混合物中其它成分的液体混合物的目标细菌。 随后将用于靶细菌的低浓度噬菌体用于感染使用IMS技术捕获的靶细菌细胞。 如果存在至少一定浓度的目标细菌，则噬菌体将增加到可检测的点。 然后使用矩阵辅助激光解吸电离/飞行时间 - 质谱（MALDI / TOF-MS）产生质谱，以分析其中是否存在与噬菌体相关的一种或多种蛋白质，从而间接表明 目标细菌存在于液体混合物中。

公开(公告)号：US20070148638A1

公开(公告)日：2007-06-28

申请号：US11612587

申请日：2006-12-19

申请人： Angelo Madonna ,  Kent Voorhees ,  Jon Rees

发明人： Angelo Madonna ,  Kent Voorhees ,  Jon Rees

IPC分类号： C12Q1/70 ,  G01N33/554

CPC分类号： C12Q1/04 ,  G01N33/54326 ,  G01N33/56911

摘要： The invention is directed to a method for detecting low concentrations of bacteria in liquid solution that may or may not be complex liquid solutions. In one embodiment, immunomagnetic separation (IMS) is used to separate target bacterium that may be in a liquid mixture from other constituents in the mixture. A low concentration of a bacteriophage for the target bacteria is subsequently used to infect target bacterial cells that have been captured using the IMS technique. If at least a certain concentration of target bacterium are present, the bacteriophage will multiply to a point that is detectable. Matrix assisted laser desorption ionization/time-of-flight-mass spectrometry (MALDI/TOF-MS) is then used to produce a mass spectrum that is analyzed to determine if one or more proteins associated with the bacteriophage are present, thereby indirectly indicating that target bacterium were present in the liquid mixture.

摘要翻译： 本发明涉及一种用于检测液体溶液中低浓度细菌的方法，该方法可能是或可能不是复杂的液体溶液。 在一个实施方案中，使用免疫磁性分离（IMS）来分离来自混合物中其它成分的液体混合物的目标细菌。 随后将用于靶细菌的低浓度噬菌体用于感染使用IMS技术捕获的靶细菌细胞。 如果存在至少一定浓度的目标细菌，则噬菌体将增加到可检测的点。 然后使用矩阵辅助激光解吸电离/飞行时间 - 质谱（MALDI / TOF-MS）产生质谱，以分析其中是否存在与噬菌体相关的一种或多种蛋白质，从而间接表明 目标细菌存在于液体混合物中。