公开(公告)号：US20070054285A1

公开(公告)日：2007-03-08

申请号：US11399310

申请日：2006-04-06

申请人： Kevin McKernan ,  Junaid Ziauddin

发明人： Kevin McKernan ,  Junaid Ziauddin

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/1006 ,  C12N15/1013

摘要： Described herein is a method in which genomic nucleic acid of a cell can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid (e.g., plasmid DNA) of the cell directly from a cell growth culture. Also described herein, a method in which genomic nucleic acid can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid in a cell lysate without the need to prepare a cleared lysate.

摘要翻译： 本文描述了一种方法，其中细胞的基因组核酸可从直接来自细胞生长培养物的细胞的分子量低于细胞的基因组核酸（例如质粒DNA）的分子量的核酸分离 。 本文还描述了可以将基因组核酸与细胞裂解物中分子量低于基因组核酸分子量的核酸分离而不需要制备澄清的裂解物的方法。

公开(公告)号：US20060078923A1

公开(公告)日：2006-04-13

申请号：US11231363

申请日：2005-09-20

申请人： Kevin McKernan ,  Junaid Ziauddin

发明人： Kevin McKernan ,  Junaid Ziauddin

IPC分类号： C12Q1/68 ,  C12N1/08

CPC分类号： C12N15/1006 ,  C12N15/1013

摘要： Described herein is a method in which genomic nucleic acid of a cell can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid (e.g., plasmid DNA) of the cell directly from a cell growth culture. Also described herein, a method in which genomic nucleic acid can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid in a cell lysate without the need to prepare a cleared lysate. The present invention is also directed to a method of isolating genomic nucleic acid (e.g., RNA, DNA) of a cell or an organism.

摘要翻译： 本文描述了一种方法，其中细胞的基因组核酸可从直接来自细胞生长培养物的细胞的分子量低于细胞的基因组核酸（例如质粒DNA）的分子量的核酸分离 。 本文还描述了可以将基因组核酸与细胞裂解物中分子量低于基因组核酸分子量的核酸分离而不需要制备澄清的裂解物的方法。 本发明还涉及分离细胞或生物体的基因组核酸（例如RNA，DNA）的方法。