公开(公告)号：US20100132059A1

公开(公告)日：2010-05-27

申请号：US12615710

申请日：2009-11-10

申请人： Kevin Michael MCCARTHY ,  Robin Allison Heller-Harrison ,  Mark William Leonard ,  Timothy Shea Charlebois

发明人： Kevin Michael MCCARTHY ,  Robin Allison Heller-Harrison ,  Mark William Leonard ,  Timothy Shea Charlebois

IPC分类号： A01K67/00 ,  C07K2/00 ,  C12N5/10 ,  C07H21/00 ,  C07H21/04 ,  C12N15/74

CPC分类号： C07K14/47 ,  C12N15/63

摘要： The present invention is based on the discovery that the transcriptional activity of both hamster mammary tumor 7 (HMT-7) and hamster layilin genes are increased when Chinese Hamster Ovary (CHO) cells are cultured at temperatures lower than 37° C. The present invention provides novel genomic, cDNA, and protein sequences of HMT-7 and a novel genomic sequence for hamster layilin. Included in the genomic sequences are novel temperature-induced promoter sequences for each; two promoter sequences are provided for HMT-7 and one promoter sequence is provided for layilin. The invention additionally provides antisense and siRNA molecules to the nucleic acid molecules of HMT-7. The invention also provides genetically engineered expression vectors comprising the novel temperature-induced promoters of the invention, which are particularly useful as part of a mammalian temperature-inducible expression system. The invention also provides host cells and/or transgenic animals comprising the novel nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention, e.g., for temperature-induced transgene expression.

摘要翻译： 本发明是基于以下发现：当在低于37℃的温度培养中国仓鼠卵巢（CHO）细胞时，仓鼠乳房肿瘤7（HMT-7）和仓鼠蛋白基因的转录活性增加。本发明 提供HMT-7的新型基因组，cDNA和蛋白质序列以及仓鼠铺板的新型基因组序列。 基因组序列中包含的每一个都是新的温度诱导的启动子序列; 为HMT-7提供了两个启动子序列，并为织物提供了一个启动子序列。 本发明还向HMT-7的核酸分子提供反义和siRNA分子。 本发明还提供了包含本发明的新型温度诱导启动子的基因工程表达载体，其特别可用作哺乳动物温度诱导表达系统的一部分。 本发明还提供了包含本发明的新型核酸分子的宿主细胞和/或转基因动物。 本发明进一步提供使用本发明的多核苷酸的方法，例如用于温度诱导的转基因表达。