公开(公告)号：US06201168B1

公开(公告)日：2001-03-13

申请号：US09378418

申请日：1999-08-20

申请人： Kevin P. Campbell ,  Ramon Coral ,  Ronald Cohn ,  Roger Williamson ,  Madeleine Durbeej

发明人： Kevin P. Campbell ,  Ramon Coral ,  Ronald Cohn ,  Roger Williamson ,  Madeleine Durbeej

IPC分类号： A01K67027

CPC分类号： C12N15/8509 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0375 ,  A61K48/00 ,  C07K14/4707

摘要： Disclosed is a mouse, cells derived therefrom, and methods for using the mouse, the mouse being homozygous for a disrupted &dgr;-sarcoglycan gene, the disruption in the gene having been introduced into the mouse or an ancestor of the mouse at an embryonic stage. The disruption prevents the synthesis of functional &dgr;-sarcoglycan in cells of the mouse and results in the mouse having a reduced amount of &bgr;- and &egr;-sarcoglycan and sarcospan, and a disruption of the sarcoglycan-sarcospan complex in smooth muscle of the mouse. Also disclosed is a mouse, cells derived therefrom, and methods for using the mouse, the mouse being homozygous for a disrupted &bgr;-sarcoglycan gene, the disruption in the gene having been introduced into the mouse or an ancestor of the mouse at an embryonic stage. The disruption prevents the synthesis of functional &bgr;-sarcoglycan in cells of the mouse and results in the mouse having a reduced amount of &dgr;-and &egr;-sarcoglycan and sarcospan and &agr;-dystroglycan in smooth muscle of the mouse.

摘要翻译： 公开了一种小鼠，其衍生的细胞，以及使用该小鼠的方法，该小鼠对于破坏的δ-色氨酸聚糖基因是纯合的，该基因在胚胎阶段被导入小鼠或该小鼠祖先的破坏。 这种破坏阻止了小鼠细胞中功能性δ-糖聚糖的合成，并导致小鼠β-谷氨酸和唾液酸和焦糖的量减少，并且导致小鼠平滑肌中的slegoglycan-sarcospan复合物的破坏。 还公开了一种小鼠，由其衍生的细胞，以及使用该小鼠的方法，该小鼠对于破坏的β-唾液酸聚糖基因是纯合的，该基因在胚胎阶段被导入小鼠或小鼠祖先的破坏 。 破坏阻止了小鼠细胞中功能性β-sogoglycan的合成，并导致小鼠的平滑肌中δ-和ε-唾液酸和谷氨酸和α-依托莫多糖的量减少。