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102 条记录 (耗时 0.034 秒)

    Conditionally immortalized cell lines derived from transgenic animals
    2.
    发明授权
    Conditionally immortalized cell lines derived from transgenic animals 失效
    衍生自转基因动物的有条件的永生化细胞系

    公开(公告)号：US06825394B1

    公开(公告)日：2004-11-30

    申请号：US09173821

    申请日：1998-10-16

    申请人： Philip Spencer Rudland ,  Barry Roger Barraclough ,  Iain Charles Kilty ,  Barry Robert Davies ,  Guenter Schmidt

    发明人： Philip Spencer Rudland ,  Barry Roger Barraclough ,  Iain Charles Kilty ,  Barry Robert Davies ,  Guenter Schmidt

    IPC分类号： A01K67027

    CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/10 ,  A01K2227/105 ,  A01K2267/0331 ,  A01K2267/0393 ,  C12N2510/04 ,  C12N2517/02

    摘要： The invention relates to mammalian cell lines and transgenic mammals. More particularly, it relates to a method for producing a rat cell line, a method for producing a transgenic rat, a transgenic rat, a rat cell line, cells and tissue obtained therefrom and uses therefore. The cell line derived from a transgenic mammal comprises: (i) a conditional oncogene, transforming gene or immortalising gene or a cell cycle affecting gene; and (ii) a cell type specific promoter. They include a neuronal cell line in which the cell type specific promoter is an NF-L gene promoter, and a mammary cell line in which the cell type specific promoter is a MMTV gene promoter. The conditional oncogene, transforming gene or immortalising gene is preferably a SV40tsA58 gene.

    摘要翻译： 本发明涉及哺乳动物细胞系和转基因哺乳动物。 更具体地说，本发明涉及生产大鼠细胞系的方法，转基因大鼠的制备方法，转基因大鼠，大鼠细胞系，由此获得的细胞和组织，因此可以使用。 衍生自转基因哺乳动物的细胞系包括：（i）条件致癌基因，转化基因或永生化基因或影响细胞周期的基因; 和（ii）细胞类型特异性启动子。 它们包括其中细胞类型特异性启动子是NF-L基因启动子的神经元细胞系和其中细胞类型特异性启动子是MMTV基因启动子的乳腺细胞系。 条件致癌基因，转化基因或永生化基因优选为SV40tsA58基因。

    Transgenic mice containing retina-specific nuclear receptor gene disruptions
    5.
    发明授权
    Transgenic mice containing retina-specific nuclear receptor gene disruptions 失效
    含有视网膜特异性核受体基因破坏的转基因小鼠

    公开(公告)号：US06730821B2

    公开(公告)日：2004-05-04

    申请号：US09811361

    申请日：2001-03-16

    申请人： Catherine Guenther

    发明人： Catherine Guenther

    IPC分类号： A01K67027

    CPC分类号： C12N15/8509 ,  A01K67/0276 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2217/20 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0393 ,  C12N15/85

    摘要： The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising disruptions in genes, which are useful as models for disease and for identifying agents that modulate gene expression and gene function, and as potential treatments for various disease states and disease conditions.

    摘要翻译： 本发明涉及转基因动物，以及与基因功能表征相关的组合物和方法。 具体地，本发明提供了包含基因中断的转基因小鼠，其可用作疾病模型和用于鉴定调节基因表达和基因功能的试剂，以及作为各种疾病状态和疾病状况的潜在治疗。

    Self-contained system for sustained viral replication
    7.
    发明授权
    Self-contained system for sustained viral replication 失效
    支持脾细胞中HIV-1复制的转基因小鼠

    公开(公告)号：US06563014B2

    公开(公告)日：2003-05-13

    申请号：US09460555

    申请日：1999-12-14

    申请人： Harris Goldstein ,  Jessie Browning Paul

    发明人： Harris Goldstein ,  Jessie Browning Paul

    IPC分类号： A01K67027

    CPC分类号： A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/0337 ,  C07K14/70514 ,  C07K14/7158 ,  C12N15/8509

    摘要： Transgenic non-human animals are described comprising a transgene for a species-specific pathogen and transgene(s) for at least one receptor restricting infection of the pathogen to the host species. Also described is a method for creating the transgenic non-human animal of this invention and a method for screening an agent for the ability to inhibit infection by a species-specific virus using said transgenic non-human animal. The transgenic animal of this invention has a sustained productive viral infection and does not develop a virus-specific immune response, thereby resulting in an extremely useful self-contained system to investigate the factors modulating in vivo replication of human pathogens, the pathophysiological effect of pathogen replication and production, and the effectiveness of novel therapies and vaccines modifying or inhibiting the course of pathogenesis.

    摘要翻译： 描述了转基因非人动物，其包含用于物种特异性病原体的转基因和限制宿主物种感染病原体的至少一种受体的转基因。 还描述了用于产生本发明的转基因非人动物的方法和用于筛选药物以抑制由所述转基因非人动物的物种特异性病毒感染的能力的方法。 本发明的转基因动物具有持续的生产性病毒感染并且不产生病毒特异性免疫应答，由此导致非常有用的自体系统来研究调节人病原体体内复制的因子，病原体的病理生理学作用 复制和生产，以及修饰或抑制发病过程的新疗法和疫苗的有效性。

    Transgenic mice deficient in natural killer cells
    8.
    发明授权
    Transgenic mice deficient in natural killer cells 失效
    缺乏自然杀伤细胞的转基因小鼠

    公开(公告)号：US06548737B1

    公开(公告)日：2003-04-15

    申请号：US09312454

    申请日：1999-05-14

    申请人： Wayne M. Yokoyana ,  Sungjin Kim

    发明人： Wayne M. Yokoyana ,  Sungjin Kim

    IPC分类号： A01K67027

    CPC分类号： G01N33/5088

    摘要： A mouse is provided which is selectively deficient in natural killer cells. Also provided are methods for producing the mouse. Additionally, mice which are deficient in natural killer cells and B cells, natural killer cells and T cells, and natural killer cells, T cells, and B cells are provided, along with methods for making these mice. Methods of using these mice to: produce an animal containing human tissue, evaluate the effects of a composition on human tissue, determine whether a human disease is caused by a pathogenic agent, and study natural killer cells are also provided.

    摘要翻译： 提供了选择性缺乏天然杀伤细胞的小鼠。 还提供了用于生产鼠标的方法。 此外，提供缺乏天然杀伤细胞和B细胞，天然杀伤细胞和T细胞的小鼠，以及天然杀伤细胞，T细胞和B细胞以及制备这些小鼠的方法。 使用这些小鼠的方法：产生含有人体组织的动物，评估组合物对人体组织的影响，确定人类疾病是否由病原体引起，并且还提供研究天然杀伤细胞。

    Pancreatic &bgr; cell hexokinase transgene
    9.
    发明授权
    Pancreatic &bgr; cell hexokinase transgene 失效
    胰腺β细胞己糖激酶转基因

    公开(公告)号：US06486380B1

    公开(公告)日：2002-11-26

    申请号：US08169613

    申请日：1993-12-17

    申请人： Paul N. Epstein

    发明人： Paul N. Epstein

    IPC分类号： A01K67027

    CPC分类号： C12N15/85 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/0306 ,  C07K14/62 ,  C12N9/1205 ,  C12N15/8509 ,  C12N2830/008 ,  C12N2830/85 ,  C12N2840/44

    摘要： A transgenic animal containing germ cells and somatic cells which contain a non-native recombinant gene which codes upon expression hexokinase capable of expressing hexokinase at significantly increased levels for study of the role of hexokinase in diabetes and other diseases. When these transgenic animals were exposed to a calmodulin stimulated model of diabetes increased levels of hexokinase activity were able to promote insulin secretion thereby reducing glucose levels in blood and transiently reduce the effects of diabetes.

    摘要翻译： 含有生殖细胞和体细胞的转基因动物，其含有非天然重组基因，其编码能够以显着增加的水平表达己糖激酶的表达己糖激酶，以研究己糖激酶在糖尿病和其他疾病中的作用。 当这些转基因动物暴露于钙调素刺激的糖尿病模型时，己糖激酶活性水平的升高能够促进胰岛素分泌，从而降低血液中的葡萄糖水平并短暂降低糖尿病的作用。

    Expression of active human factor IX in mammary tissue and of milk non human transgenic mammals
    10.
    发明授权
    Expression of active human factor IX in mammary tissue and of milk non human transgenic mammals 失效
    活性人因子IX在转基因非人哺乳动物的乳腺组织和乳中的表达

    公开(公告)号：US06344596B1

    公开(公告)日：2002-02-05

    申请号：US09367087

    申请日：1999-09-15

    申请人： William H. Velander ,  William N. Drohan ,  Henryk Lubon ,  John L. Johnson

    发明人： William H. Velander ,  William N. Drohan ,  Henryk Lubon ,  John L. Johnson

    IPC分类号： A01K67027

    CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2227/108 ,  A01K2267/01 ,  A01K2267/03 ,  A61K38/36 ,  C12N9/644 ,  C12Y304/21022

    摘要： Recombinant Factor IX characterized by a high percentage of active protein can be obtained in the milk of transgenic animals that incorporate chimeric DNA molecules according to the present invention. Transgenic animals of the present invention are produced by introducing into developing embryos DNA that encodes Factor IX, such that the foreign DNA is stably incorporated in the DNA of germ line cells of the mature animal. Particularly efficient expression was accomplished using a chimeric construct comprising a mammary gland specific promoter, Factor IX cDNA that lacked the complete or any portion of the 5′-untranslated and 3′-untranslated region, which is substituted with a 5′- and 3′-end of the mouse whey acidic protein gene. In vitro cell cultures of cells explanted from the transgenic mammal of the invention and methods of producing Factor IX from such said culture and methods of treating hemophilia B are also described.

    摘要翻译： 可以在掺入根据本发明的嵌合DNA分子的转基因动物的乳中获得以高百分比的活性蛋白为特征的重组因子IX。 本发明的转基因动物通过引入编码因子IX的发育中的胚胎DNA而产生，使得外源DNA稳定地并入成熟动物的种系细胞的DNA中。 使用含有乳腺特异性启动子的嵌合构建体，缺乏5'和非翻译区的完整或任何部分的因子IX cDNA完成了特别有效的表达，该5'和3'非翻译区用5'和3' -end的小鼠乳清酸性蛋白基因。 还描述了从本发明的转基因哺乳动物移出的细胞的体外细胞培养物和从所述培养物生产因子IX的方法和治疗血友病B的方法。