公开(公告)号：US20070202084A1

公开(公告)日：2007-08-30

申请号：US11638103

申请日：2006-12-13

申请人： Khalid Sadozai ,  Carol Joth ,  Charles Sherwood

发明人： Khalid Sadozai ,  Carol Joth ,  Charles Sherwood

IPC分类号： A61K48/00 ,  A61K35/12 ,  A61K31/728

CPC分类号： A61K31/728 ,  A61K9/0019 ,  A61K9/0024 ,  A61K9/06 ,  A61K31/573 ,  A61K31/729 ,  A61K35/28 ,  A61K35/32 ,  A61K38/1841 ,  A61K45/06 ,  A61K47/36 ,  A61L27/20 ,  A61L27/227 ,  A61L27/3817 ,  A61L27/3834 ,  A61L27/3843 ,  A61L27/48 ,  A61L27/52 ,  A61L27/56 ,  A61L2430/06 ,  A61M5/178 ,  C08L5/08 ,  A61K2300/00

摘要： A method for treating an osteochondral defect or a chondral defect in a subject includes implanting a composite in a site of the osteochondral or chondral defect. The composite includes a hyaluronic acid derivative; and at least one member of the group consisting of a cell, a cellular growth factor and a cellular differentiation factor, which is impregnated in, or coupled to, the hyaluronic acid derivative. In one embodiment, carboxyl functionalities of the hyaluronic acid derivative are each independently derivatized to include an N-acylurea or O-acyl isourea, or both N-acylurea and O-acyl isourea. In another embodiment, the hyaluronic acid derivative is prepared by reacting an uncrosslinked hyaluronic acid with a biscarbodimide in the presence of a pH buffer in a range of between about 4 and about 8. The composite can be used for regenerating or stimulating regeneration of meniscal tissues in a subject in need thereof.

摘要翻译： 用于治疗受试者的骨软骨缺损或软骨缺损的方法包括在骨软骨或软骨缺损的部位植入复合物。 该复合物包括透明质酸衍生物; 和由透明质酸衍生物浸渍或偶联的细胞，细胞生长因子和细胞分化因子组成的组中的至少一个成员。 在一个实施方案中，透明质酸衍生物的羧基官能度各自独立地衍生化为包括N-酰基脲或O-酰基异脲，或N-酰基脲和O-酰基异脲二者。 在另一个实施方案中，透明质酸衍生物通过在约4至约8范围内的pH缓冲剂存在下使未交联的透明质酸与双碳酰亚胺反应来制备。该复合物可用于再生或刺激半月板组织的再生 在有需要的受试者中。