公开(公告)号：US10151726B2

公开(公告)日：2018-12-11

申请号：US14120373

申请日：2014-05-14

申请人： King Saud University

发明人： Khalid M Abu-Salah ,  Salman A. Alrokayan ,  Abdullah M. I. Mashhour

IPC分类号： G01N27/447

摘要： Systems and methods for labeling, staining, and bioimaging of nucleic acids, their fragments, proteins, polypeptides and enzymes are described. In one aspect, a percentage concentration agarose gel or polyacrylamide gel is generated with agarose or polyacrylamide powder respectively. Silicon nanoparticles are added to the agarose or polyacrylamide gel at a required concentration via the agarose or polyacrylamide powder or after agarose or polyacrylamide solubilization in a loading buffer. The nucleic acid is added to agarose gel slots and proteins or enzymes are added to polyacrylamide gel slots in the loading buffer. The loading buffer is then electrophoresed in each case for an amount of time, causing the added silicon nanoparticles or the added ethidium bromide (after agarose solubilization) to generate a bound and labeled nucleic acid and their fragments and proteins or enzymes (with silicon nanoparticles only).

公开(公告)号：US20140339083A1

公开(公告)日：2014-11-20

申请号：US14120373

申请日：2014-05-14

申请人： King Saud University

发明人： Khalid M. Abu-Salah ,  Salman A. Alrokayan ,  Abdullah M.I. Mashhour

IPC分类号： G01N27/447

CPC分类号： G01N27/44747 ,  G01N27/44721 ,  G01N27/44726

摘要： Systems and methods for labeling, staining, and bioimaging of nucleic acids, their fragments, proteins, polypeptides and enzymes are described. In one aspect, a percentage concentration agarose gel or polyacrylamide gel is generated with agarose or polyacrylamide powder respectively. Silicon nanoparticles are added to the agarose or polyacrylamide gel at a required concentration via the agarose or polyacrylamide powder or after agarose or polyacrylamide solubilization in a loading buffer. The nucleic acid is added to agarose gel slots and proteins or enzymes are added to polyacrylamide gel slots in the loading buffer. The loading buffer is then electrophoresed in each case for an amount of time, causing the added silicon nanoparticles or the added ethidium bromide (after agarose solubilization) to generate a bound and labeled nucleic acid and their fragments and proteins or enzymes (with silicon nanoparticles only).

摘要翻译： 描述了核酸，其片段，蛋白质，多肽和酶的标记，染色和生物成像的系统和方法。 在一个方面，分别用琼脂糖或聚丙烯酰胺粉末产生百分浓度琼脂糖凝胶或聚丙烯酰胺凝胶。 将硅纳米颗粒通过琼脂糖或聚丙烯酰胺粉末以琼脂糖或聚丙烯酰胺溶解于加料缓冲液中，以所需浓度加入到琼脂糖或聚丙烯酰胺凝胶中。 将核酸加入到琼脂糖凝胶槽中，并将蛋白质或酶加入到加载缓冲液中的聚丙烯酰胺凝胶槽中。 然后将负载缓冲液在每种情况下电泳一段时间，使加入的硅纳米颗粒或加入的溴化乙锭（在琼脂糖溶解后）产生结合和标记的核酸及其片段和蛋白质或酶（仅含硅纳米颗粒 ）。