Biosensor Utilizing Pigment-Synthesizing Gene Of A Purple Non-Sulfur Bacterium And A Method For Preparing Such A Biosensor
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    发明申请
    Biosensor Utilizing Pigment-Synthesizing Gene Of A Purple Non-Sulfur Bacterium And A Method For Preparing Such A Biosensor 审中-公开
    使用紫色非硫细菌的颜料合成基因的生物传感器和制备这种生物传感器的方法

    公开(公告)号:US20080044829A1

    公开(公告)日:2008-02-21

    申请号:US11660588

    申请日:2005-08-22

    IPC分类号: C12Q1/68

    摘要: A biosensor where measurement is able to be conducted by simpler operations, cost is less expensive and specific chemical substance is able to be visually detected is provided. In accordance with the present invention, there is provided a biosensor for the detection of a specific chemical substance, characterized in that it comprises a recombinant purple non-sulfur bacterium wherein sensor vector is introduced into a pigment variant of a purple non-sulfur bacterium being deficient in gene coding for a spheroidene monooxygenase enzyme, and that the sensor vector is a sensor vector where inductive promoter showing a specific response to a specific chemical substance is connected in an operable manner to the upstream region of gene coding for the spheroidene monooxygenase enzyme.

    摘要翻译: 可以通过简单的操作进行测量的生物传感器,成本更低,并且可以在视觉上检测特定的化学物质。 根据本发明,提供了用于检测特定化学物质的生物传感器,其特征在于,其包含重组紫色非硫细菌,其中将传感器载体引入紫色非硫细菌的颜料变体中 并且传感器载体是一种传感器载体,其中显示对特定化学物质的特异性响应的归纳启动子以可操作的方式连接到编码球状体单加氧酶的基因的上游区域。