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    Detergent comprising isolated cellulase from bacillus ferm bp-3431 or a mutant strain thereof, surfactant and builder
    1.
    发明授权
    Detergent comprising isolated cellulase from bacillus ferm bp-3431 or a mutant strain thereof, surfactant and builder 失效
    洗涤剂包括来自芽孢杆菌bp-3431的分离的纤维素酶或其突变株，表面活性剂和助洗剂

    公开(公告)号：US5314637A

    公开(公告)日：1994-05-24

    申请号：US29623

    申请日：1993-03-11

    申请人： Kiyoshi Saito ,  Masahiko Seko ,  Eiko Masatsuji

    发明人： Kiyoshi Saito ,  Masahiko Seko ,  Eiko Masatsuji

    IPC分类号： C11D3/386 ,  C12N1/21 ,  C12N9/42 ,  C12N15/56 ,  C12R1/07 ,  D21C5/00 ,  C11D17/00 ,  C12N1/00 ,  C12N1/20

    CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12Y302/01004 ,  D21C5/005 ,  Y10S435/832

    摘要： A cellulase having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30.degree. C. and for 30 minutes; (3) an optimum temperature of about 55.degree. C. as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90% or more after treatment at 30.degree. C. and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000.+-.2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2.+-.0.2 as measured by isoelectric electrophoresis. The cellulase is produced by a Bacillus strain FERM BP-3431 or a mutant strain thereof. A detergent comprising the cellulase, a builder and a surfactant is also disclosed.

    摘要翻译： 具有以下特性的纤维素酶：（1）使用羧甲基纤维素作为底物测定的最佳pH为9.5至10.5; （2）在30℃下处理30分钟后，使用羧甲基纤维素作为底物测定的稳定pH为6〜11; （3）使用羧甲基纤维素作为底物测定的约55℃的最适温度; （4）表面活性剂的影响使得在正烷基苯磺酸钠存在下，在30℃，pH9.0下处理2小时后的残留活性为90％以上; （5）通过在SDS-聚丙烯酰胺凝胶上电泳测得的分子量为52,000 +/- 2,000; 和（6）通过等电泳测定的等电点为4.2 +/- 0.2。 纤维素酶由芽孢杆菌菌株FERM BP-3431或其突变菌株产生。 还公开了包含纤维素酶，助洗剂和表面活性剂的洗涤剂。

    Composition containing celluase from Bacillus Ferm BP-3431 or a mutant strain thereof, and paper pulp and method of using celluase to treat paper pulp slurry
    2.
    发明授权
    Composition containing celluase from Bacillus Ferm BP-3431 or a mutant strain thereof, and paper pulp and method of using celluase to treat paper pulp slurry 失效
    含有来自Bacillus Ferm BP-3431或其突变株的纤维素酶的组合物，以及纸浆和使用纤维素酶处理纸浆的方法

    公开(公告)号：US5318905A

    公开(公告)日：1994-06-07

    申请号：US59169

    申请日：1993-03-11

    申请人： Kiyoshi Saito ,  Masahiko Seko ,  Eiko Masatsuji

    发明人： Kiyoshi Saito ,  Masahiko Seko ,  Eiko Masatsuji

    IPC分类号： C11D3/386 ,  C12N1/21 ,  C12N9/42 ,  C12N15/56 ,  C12R1/07 ,  D21C5/00 ,  C12N1/00 ,  C12N1/22 ,  D21F11/00

    CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12Y302/01004 ,  D21C5/005 ,  Y10S435/832

    摘要： A cellulase is provided having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30.degree. C. and for 30 minutes; (3) an optimum temperature of about 55.degree. C. as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90% or more after treatment at 30.degree. C. and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000.+-.2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2.+-.0.2 as measured by isoelectric electrophoresis. The cellulase is produced by Bacillus FERM BP-3431 or a mutant strain thereof. Also disclosed is a detergent comprising the cellulase, a builder and a surfactant. A composition and a method of treating a paper pulp slurry is also disclosed.

    摘要翻译： 提供具有以下特性的纤维素酶：（1）使用羧甲基纤维素作为底物测定的最佳pH为9.5至10.5; （2）在30℃下处理30分钟后，使用羧甲基纤维素作为底物测定的稳定pH为6〜11; （3）使用羧甲基纤维素作为底物测定的约55℃的最适温度; （4）表面活性剂的影响使得在正烷基苯磺酸钠存在下，在30℃，pH9.0下处理2小时后的残留活性为90％以上; （5）通过在SDS-聚丙烯酰胺凝胶上电泳测得的分子量为52,000 +/- 2,000; 和（6）通过等电泳测定的等电点为4.2 +/- 0.2。 纤维素酶由芽孢杆菌FERM BP-3431或其突变株产生。 还公开了包含纤维素酶，助洗剂和表面活性剂的洗涤剂。 还公开了一种处理纸浆浆料的组合物和方法。

    Cellulase isolated from Bacillus ferm BP-3431 or a mutant strain thereof
    3.
    发明授权
    Cellulase isolated from Bacillus ferm BP-3431 or a mutant strain thereof 失效
    从BACILLUS FERM BP-3431或其突变菌株分离的纤维素酶

    公开(公告)号：US5231022A

    公开(公告)日：1993-07-27

    申请号：US734662

    申请日：1991-07-23

    申请人： Kiyoshi Saito ,  Masahiko Seko ,  Eiko Masatsuji

    发明人： Kiyoshi Saito ,  Masahiko Seko ,  Eiko Masatsuji

    IPC分类号： C11D3/386 ,  C12N1/21 ,  C12N9/42 ,  C12N15/56 ,  C12R1/07 ,  D21C5/00

    CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12Y302/01004 ,  D21C5/005 ,  Y10S435/832

    摘要： A cellulase having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30.degree. C. and for 30 minutes; (3) an optimum temperature of about 55.degree. C. as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90% or more after treatment at 30.degree. C. and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000.+-.2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2.+-.0.2 as measured by isoelectric electrophoresis. The cellulase is produced by Bacillus sp. SD402, its mutant strains or its genetic engineered strains. An aid for detergents and a paper treating agent, respectively, contain the cellulase as an effective ingredient.