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1.发明授权Quantitative analysis of 3.alpha.-hydroxysteroid and reagent useful therefor 失效3α-羟基类固醇及其有用的试剂的定量分析
公开(公告)号：US4816394A
公开(公告)日：1989-03-28
申请号：US861725
申请日：1986-05-12
CPC分类号： C12Q1/32
摘要： A 3.alpha.-hydroxysteroid contained in a sample can be quantitatively analyzed by causing a 3.alpha.-hydroxysteroid dehydrogenase to act on the sample in the presence of nicotinamide adenine dinucleotide, causing tetrazolium or a salt thereof, an electron carrier substance, and 3-oxosteroid-.DELTA..sup.1 -dihydrogenase and/or 3-oxosteroid-.DELTA..sup.4 -dehydrogenase to act on the thus-obtained reaction product, and then quantitatively analyzing the resultant formazan.
摘要翻译： 通过使3α-羟基类固醇脱氢酶在烟酰胺腺嘌呤二核苷酸存在下作用于样品，引起四唑或其盐，电子载体物质和3-氧化甾体，可以定量分析样品中含有的3α-羟基固醇类 - DELTA 1-二氢化酶和/或3-氧代甾体-DTA4-脱氢酶作用于由此获得的反应产物，然后定量分析所得的甲an。
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2.发明授权Method and reagent for quantitative analysis of 3-oxo-5.beta.-steroid 失效3-OXO-5（BETA）-STEROID的定量分析方法和试剂
公开(公告)号：US5085989A
公开(公告)日：1992-02-04
申请号：US574525
申请日：1990-08-28
申请人： Koji Ushizawa , Takae Shigihara , Maki Akemichi , Toshiyuki Akimoto , Senkichi Nagasaki , Miyoshi Hirata
发明人： Koji Ushizawa , Takae Shigihara , Maki Akemichi , Toshiyuki Akimoto , Senkichi Nagasaki , Miyoshi Hirata
IPC分类号： C12Q1/32
CPC分类号： C12Q1/32
摘要： A quantitative analysis of 3-oxo-5.beta.-steroid is carried out by acting 3-oxo-5.beta.-steroid.DELTA..sup.4 -dehydrogenase on a sample in the presence of a reducing chromophoric agent, and measuring the optical density of the chromophoric substance thereby produced. A typical reducing chromophoric agent is a tetrazolium compound. The invention also provides a reagent for the quantitative analysis of 3-oxo-5.beta.-steroid comprising 3-oxo-5.beta.-steroid.DELTA..sup.4 -dehydrogenase and a tetrazolium compound. The analysis and reagent provide a simple and reliable method for a liver function test.
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