公开(公告)号：US20230287073A1

公开(公告)日：2023-09-14

申请号：US18147796

申请日：2022-12-29

申请人： Korea Institute of Ocean Science & Technology

发明人： Jung-Hyun LEE ,  Hyung-Soon YIM ,  Young Jun AN ,  Kyeong Won LEE ,  Ye Eun JEONG ,  Kiweon CHA ,  Won Kyu LEE

IPC分类号： C07K14/50

CPC分类号： C07K14/50

摘要： A thermally stable polypeptide having FGF7 activity is provided. The polypeptide is a thermally stable polypeptide having FGF7 activity, wherein, in SEQ ID NO: 1, a 120th alanine (A) is substituted with cysteine (C), one including at least one substitution selected from substitution of a 126th lysine (K) with aspartic acid (D) and a 178th lysine (K) with glutamic acid (E) or aspartic acid (D) forms a salt bridge with a 175th arginine (R), and a 133rd cysteine (C) and a 137th cysteine (C) are disulfide bonded.

公开(公告)号：US20210355182A1

公开(公告)日：2021-11-18

申请号：US16997198

申请日：2020-08-19

申请人： INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY ,  KOREA INSTITUTE OF OCEAN SCIENCE & TECHNOLOGY

发明人： Geun Joong KIM ,  Hye Ji CHOI ,  Dae Eun CHEONG ,  Su Kyoung YOO ,  Dong Hyun LEE ,  Jae Hong PARK ,  Jung Hyun LEE ,  Hyung Soon YIM ,  Young Jun AN ,  Kyeong Won LEE

IPC分类号： C07K14/50 ,  C12N9/90 ,  A61K38/00

摘要： A recombinant vector according to an embodiment of the present invention may produce fibroblast growth factor 19 (FGF19) having enhanced solubility. The synonymous codon substitution variant fibroblast growth factor 19 (scvhFGF19) and chaperone ΔssDsbC may be simultaneously and independently expressed in a host cell into which the recombinant vector is introduced, thereby it is possible to overexpress the fibroblast growth factor 19 in a soluble state, due to when not utilizing tags such as an existing fusion partner there is no change in the amino acid, which is a problem generated in the removal process.