公开(公告)号：US11592415B2

公开(公告)日：2023-02-28

申请号：US16070672

申请日：2016-10-20

Applicant: Korea Institute of Science and Technology

Inventor： Kyo Seon Hwang ,  Young Soo Kim ,  Jin Sik Kim ,  Yong Kyoung Yoo

IPC: G01N27/327 ,  G01N27/12 ,  G01N33/68 ,  G01N33/543 ,  C08L39/06 ,  C08L83/04

Abstract: The present invention relates to an interdigitated microelectrode biosensor using the reaction between receptors and target biomaterials, the interdigitated microelectrode biosensor comprising: an insulating layer formed so as to cover all of the sensor formation area of a substrate; a first interdigitated microelectrode formed such that a plurality of first protruding electrodes are arranged in a comb shape on the insulating layer of the substrate; a second interdigitated microelectrode, facing the first interdigitated microelectrode and formed such that a plurality of second protruding electrodes are arranged in a comb shape on the insulating layer of the substrate such that the plurality of second protruding electrodes are arranged to respectively interdigitate with the plurality of first protruding electrodes formed at the first interdigitated microelectrode; and a plurality of receptors arranged in the space between the first and second interdigitated microelectrodes, which are arranged to interdigitate with each other, so as to specifically react with the target biomaterial, thereby increasing an impedance detection width and detection limit, and improving detection accuracy according to the characteristics of each monomer and each polymer.

公开(公告)号：US10281419B2

公开(公告)日：2019-05-07

申请号：US15105740

申请日：2016-03-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Kyoseon Hwang ,  Nakwon Choi ,  Jinsik Kim ,  Hye Jin Kim ,  Yong Kyoung Yoo ,  Sang Yun Yeom ,  Woongsun Choi

IPC: G01N27/12 ,  G01N33/53 ,  G01N33/483 ,  G01N33/487 ,  G01N33/543

Abstract: A hydrogel-based interdigitated microelectrode biosensor is disclosed. The hydrogel-based interdigitated microelectrode biosensor includes: a first interdigitated microelectrode having a plurality of first protrusion electrodes arranged in a comb-like shape on a substrate; a second interdigitated microelectrode facing the first interdigitated microelectrode and having a plurality of second protrusion electrodes arranged in a comb-like shape on the substrate, the second protrusion electrodes being arranged alternately with the first protrusion electrodes of the first interdigitated microelectrode; and a hydrogel filled in a space between the first and second interdigitated microelectrodes arranged alternately with each other. The hydrogel is provided between the interdigitated microelectrodes such that the presence and concentration of a biological substance, such as a protein, are detected by measuring the impedance between the interdigitated microelectrodes.

公开(公告)号：US11313831B2

公开(公告)日：2022-04-26

申请号：US16077074

申请日：2016-11-09

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Kyoseon Hwang ,  Jinsik Kim ,  Hye Jin Kim ,  Yong Kyoung Yoo ,  Youngsoo Kim

IPC: B03C5/00 ,  G01N33/543 ,  G01N27/447 ,  B03C5/02

Abstract: An interdigitated electrode biosensor includes an insulating layer configured to fully cover a sensor forming region of a substrate, a first interdigitated microelectrode configured such that a plurality of first protruding electrodes is arranged in a shape of a comb on the substrate, a second interdigitated microelectrode configured such that a plurality of second protruding electrodes is arranged in a shape of a comb and each interdigitates with the plurality of first protruding electrodes, and a plurality of receptors that is immobilized in a space between the first interdigitated microelectrode and the second interdigitated microelectrode and reacts specifically to target biomaterials. Different voltages are uniformly or nonuniformly applied to the first interdigitated microelectrode and the second interdigitated microelectrode to generate a dielectrophoretic force by a nonuniform electric field, improving the sensor by increasing the probability of specific reaction with the target biomaterials using the concentration effect through dielectrophoresis.